Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf
Showy lady's slipper (Cypripedium reginae Walter, Orchidaceae) and black ash (Fraxinus nigra Marshall, Oleaceae) often co-occur in close proximity in fens in western Newfoundland, Canada. Metabarcoding of DNA extracted from root samples of both species following surface sterilization, and other...
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ftfrontimediafig:oai:figshare.com:article/19254242 2023-05-15T17:22:29+02:00 Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf Nimalka M. Weerasuriya Katarina Kukolj Rebecca Spencer Dmitry Sveshnikov R. Greg Thorn 2022-03-01T04:43:39Z https://doi.org/10.3389/ffunb.2022.805127.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Multiple_Fungi_May_Connect_the_Roots_of_an_Orchid_Cypripedium_reginae_and_Ash_Fraxinus_nigra_in_Western_Newfoundland_pdf/19254242 unknown doi:10.3389/ffunb.2022.805127.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Multiple_Fungi_May_Connect_the_Roots_of_an_Orchid_Cypripedium_reginae_and_Ash_Fraxinus_nigra_in_Western_Newfoundland_pdf/19254242 CC BY 4.0 CC-BY Genomics Mycology showy lady's slipper orchid black ash mycorrhiza metabarcoding Illumina MiSeq mixotrophy common mycorrhizal network ITS2 Dataset 2022 ftfrontimediafig https://doi.org/10.3389/ffunb.2022.805127.s001 2022-03-03T00:03:27Z Showy lady's slipper (Cypripedium reginae Walter, Orchidaceae) and black ash (Fraxinus nigra Marshall, Oleaceae) often co-occur in close proximity in fens in western Newfoundland, Canada. Metabarcoding of DNA extracted from root samples of both species following surface sterilization, and others without surface sterilization was used to determine if there were shared fungal endophytes in the roots of both species that could form a common mycorrhizal network between them. A wide variety of fungi were recovered from primers amplifying the nuclear ribosomal internal transcribed spacer region (ITS2). Sixty-six fungal sequences were shared by surface-sterilized roots of both orchid and ash, among them arbuscular mycorrhizal fungi (Claroideoglomus, Dominikia, Glomus and Rhizophagus), ectomycorrhizal fungi (Inocybe and Tomentella), the broad-host root endophyte Cadophora orchidicola, along with root pathogens (Dactylonectria, Ilyonectria, Pyricularia, and Xylomyces) and fungi of unknown function. There appear to be multiple fungi that could form a common mycorrhizal network between C. reginae and F. nigra, which might explain their frequent co-occurrence. Transfer of nutrients or carbon between the orchid and ash via one or more of the shared fungal endophytes remains to be demonstrated. Dataset Newfoundland Frontiers: Figshare Canada |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Genomics Mycology showy lady's slipper orchid black ash mycorrhiza metabarcoding Illumina MiSeq mixotrophy common mycorrhizal network ITS2 |
spellingShingle |
Genomics Mycology showy lady's slipper orchid black ash mycorrhiza metabarcoding Illumina MiSeq mixotrophy common mycorrhizal network ITS2 Nimalka M. Weerasuriya Katarina Kukolj Rebecca Spencer Dmitry Sveshnikov R. Greg Thorn Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf |
topic_facet |
Genomics Mycology showy lady's slipper orchid black ash mycorrhiza metabarcoding Illumina MiSeq mixotrophy common mycorrhizal network ITS2 |
description |
Showy lady's slipper (Cypripedium reginae Walter, Orchidaceae) and black ash (Fraxinus nigra Marshall, Oleaceae) often co-occur in close proximity in fens in western Newfoundland, Canada. Metabarcoding of DNA extracted from root samples of both species following surface sterilization, and others without surface sterilization was used to determine if there were shared fungal endophytes in the roots of both species that could form a common mycorrhizal network between them. A wide variety of fungi were recovered from primers amplifying the nuclear ribosomal internal transcribed spacer region (ITS2). Sixty-six fungal sequences were shared by surface-sterilized roots of both orchid and ash, among them arbuscular mycorrhizal fungi (Claroideoglomus, Dominikia, Glomus and Rhizophagus), ectomycorrhizal fungi (Inocybe and Tomentella), the broad-host root endophyte Cadophora orchidicola, along with root pathogens (Dactylonectria, Ilyonectria, Pyricularia, and Xylomyces) and fungi of unknown function. There appear to be multiple fungi that could form a common mycorrhizal network between C. reginae and F. nigra, which might explain their frequent co-occurrence. Transfer of nutrients or carbon between the orchid and ash via one or more of the shared fungal endophytes remains to be demonstrated. |
format |
Dataset |
author |
Nimalka M. Weerasuriya Katarina Kukolj Rebecca Spencer Dmitry Sveshnikov R. Greg Thorn |
author_facet |
Nimalka M. Weerasuriya Katarina Kukolj Rebecca Spencer Dmitry Sveshnikov R. Greg Thorn |
author_sort |
Nimalka M. Weerasuriya |
title |
Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf |
title_short |
Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf |
title_full |
Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf |
title_fullStr |
Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf |
title_full_unstemmed |
Data_Sheet_1_Multiple Fungi May Connect the Roots of an Orchid (Cypripedium reginae) and Ash (Fraxinus nigra) in Western Newfoundland.pdf |
title_sort |
data_sheet_1_multiple fungi may connect the roots of an orchid (cypripedium reginae) and ash (fraxinus nigra) in western newfoundland.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/ffunb.2022.805127.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Multiple_Fungi_May_Connect_the_Roots_of_an_Orchid_Cypripedium_reginae_and_Ash_Fraxinus_nigra_in_Western_Newfoundland_pdf/19254242 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
doi:10.3389/ffunb.2022.805127.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Multiple_Fungi_May_Connect_the_Roots_of_an_Orchid_Cypripedium_reginae_and_Ash_Fraxinus_nigra_in_Western_Newfoundland_pdf/19254242 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/ffunb.2022.805127.s001 |
_version_ |
1766109176779505664 |