DataSheet1_Application of Psychrotolerant Antarctic Bacteria and Their Metabolites as Efficient Plant Growth Promoting Agents.PDF

• Main Authors: Michal Styczynski, Gabriel Biegniewski, Przemyslaw Decewicz, Bartosz Rewerski, Klaudia Debiec-Andrzejewska, Lukasz Dziewit
• Format: Dataset
• Language: unknown
• Published: 2022
• Subjects: Biotechnology; Biological Engineering; Genetic Engineering; Biomarkers; Biomaterials; Biomechanical Engineering; Biomedical Engineering not elsewhere classified; Synthetic Biology; Agricultural Marine Biotechnology; Bioremediation; Bioprocessing; Bioproduction and Bioproducts; Industrial Biotechnology Diagnostics (incl. Biosensors); Industrial Microbiology (incl. Biofeedstocks); Industrial Molecular Engineering of Nucleic Acids and Proteins; Industrial Biotechnology not elsewhere classified; Medical Biotechnology Diagnostics (incl. Biosensors); Medical Molecular Engineering of Nucleic Acids and Proteins; Regenerative Medicine (incl. Stem Cells and Tissue Engineering); Medical Biotechnology not elsewhere classified; Antarctica; biosurfactant; plant growth promoting bacteria; psychrotolerant bacteria; siderophore; Antarctic; Antarc*
• Online Access: https://doi.org/10.3389/fbioe.2022.772891.s001; https://figshare.com/articles/dataset/DataSheet1_Application_of_Psychrotolerant_Antarctic_Bacteria_and_Their_Metabolites_as_Efficient_Plant_Growth_Promoting_Agents_PDF/19226553

Iron is the fourth most abundant element on earth. However, its low bioavailability is a key plant-growth limiting factor. Bacteria play an important role in plant growth promotion since they produce specific secondary metabolites that may increase macro- and micronutrient accessibility in soil. Therefore, bacterial-derived iron chelators, as well as surface-active compounds, are recognised as essential to plant welfare. In this study, three cold-active Antarctic bacterial strains, i.e. Pseudomonas sp. ANT_H12B, Psychrobacter sp. ANT_H59 and Bacillus sp. ANT_WA51, were analysed. The physiological and genomic characterisation of these strains revealed their potential for plant growth promotion, reflected in the production of various biomolecules, including biosurfactants (that may lower the medium surface tension of even up to 53%) and siderophores (including ANT_H12B-produced mixed-type siderophore that demonstrated the highest production, reaching the concentration of up to 1.065 mM), increasing the availability of nutrients in the environment and neutralising fungal pathogens. Tested bacteria demonstrated an ability to promote the growth of a model plant, alfalfa, increasing shoots’ length and fresh biomass even up to 26 and 46% respectively; while their metabolites increased the bioavailability of iron in soil up to 40%. It was also revealed that the introduced strains did not disrupt physicochemical conditions and indigenous soil microbial composition, which suggests that they are promising amendments preserving the natural biodiversity of soil and increasing its fertility.