Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX
Microorganisms have evolved to colonize all biospheres, including extremely cold environments, facing several stressor conditions, mainly low/freezing temperatures. In general, terms, the strategies developed by cold-adapted microorganisms include the synthesis of cryoprotectant and stress-protectan...
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ftfrontimediafig:oai:figshare.com:article/19218165 2023-05-15T14:02:45+02:00 Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX Marcelo Baeza Sergio Zúñiga Vicente Peragallo Fernando Gutierrez Salvador Barahona Jennifer Alcaino Víctor Cifuentes 2022-02-23T04:38:46Z https://doi.org/10.3389/fmicb.2022.828536.s008 https://figshare.com/articles/dataset/Table_1_Response_to_Cold_A_Comparative_Transcriptomic_Analysis_in_Eight_Cold-Adapted_Yeasts_XLSX/19218165 unknown doi:10.3389/fmicb.2022.828536.s008 https://figshare.com/articles/dataset/Table_1_Response_to_Cold_A_Comparative_Transcriptomic_Analysis_in_Eight_Cold-Adapted_Yeasts_XLSX/19218165 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology cold-adapted yeasts Antarctic yeasts transcriptomes cold adaptation stress genes codon bias Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.828536.s008 2022-02-24T00:03:12Z Microorganisms have evolved to colonize all biospheres, including extremely cold environments, facing several stressor conditions, mainly low/freezing temperatures. In general, terms, the strategies developed by cold-adapted microorganisms include the synthesis of cryoprotectant and stress-protectant molecules, cold-active proteins, especially enzymes, and membrane fluidity regulation. The strategy could differ among microorganisms and concerns the characteristics of the cold environment of the microorganism, such as seasonal temperature changes. Microorganisms can develop strategies to grow efficiently at low temperatures or tolerate them and grow under favorable conditions. These differences can be found among the same kind of microorganisms and from the same cold habitat. In this work, eight cold-adapted yeasts isolated from King George Island, subAntarctic region, which differ in their growth properties, were studied about their response to low temperatures at the transcriptomic level. Sixteen ORFeomes were assembled and used for gene prediction and functional annotation, determination of gene expression changes, protein flexibilities of translated genes, and codon usage bias. Putative genes related to the response to all main kinds of stress were found. The total number of differentially expressed genes was related to the temperature variation that each yeast faced. The findings from multiple comparative analyses among yeasts based on gene expression changes and protein flexibility by cellular functions and codon usage bias raise significant differences in response to cold among the studied Antarctic yeasts. The way a yeast responds to temperature change appears to be more related to its optimal temperature for growth (OTG) than growth velocity. Yeasts with higher OTG prepare to downregulate their metabolism to enter the dormancy stage. In comparison, yeasts with lower OTG perform minor adjustments to make their metabolism adequate and maintain their growth at lower temperatures. Dataset Antarc* Antarctic King George Island Frontiers: Figshare Antarctic King George Island |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology cold-adapted yeasts Antarctic yeasts transcriptomes cold adaptation stress genes codon bias |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology cold-adapted yeasts Antarctic yeasts transcriptomes cold adaptation stress genes codon bias Marcelo Baeza Sergio Zúñiga Vicente Peragallo Fernando Gutierrez Salvador Barahona Jennifer Alcaino Víctor Cifuentes Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology cold-adapted yeasts Antarctic yeasts transcriptomes cold adaptation stress genes codon bias |
description |
Microorganisms have evolved to colonize all biospheres, including extremely cold environments, facing several stressor conditions, mainly low/freezing temperatures. In general, terms, the strategies developed by cold-adapted microorganisms include the synthesis of cryoprotectant and stress-protectant molecules, cold-active proteins, especially enzymes, and membrane fluidity regulation. The strategy could differ among microorganisms and concerns the characteristics of the cold environment of the microorganism, such as seasonal temperature changes. Microorganisms can develop strategies to grow efficiently at low temperatures or tolerate them and grow under favorable conditions. These differences can be found among the same kind of microorganisms and from the same cold habitat. In this work, eight cold-adapted yeasts isolated from King George Island, subAntarctic region, which differ in their growth properties, were studied about their response to low temperatures at the transcriptomic level. Sixteen ORFeomes were assembled and used for gene prediction and functional annotation, determination of gene expression changes, protein flexibilities of translated genes, and codon usage bias. Putative genes related to the response to all main kinds of stress were found. The total number of differentially expressed genes was related to the temperature variation that each yeast faced. The findings from multiple comparative analyses among yeasts based on gene expression changes and protein flexibility by cellular functions and codon usage bias raise significant differences in response to cold among the studied Antarctic yeasts. The way a yeast responds to temperature change appears to be more related to its optimal temperature for growth (OTG) than growth velocity. Yeasts with higher OTG prepare to downregulate their metabolism to enter the dormancy stage. In comparison, yeasts with lower OTG perform minor adjustments to make their metabolism adequate and maintain their growth at lower temperatures. |
format |
Dataset |
author |
Marcelo Baeza Sergio Zúñiga Vicente Peragallo Fernando Gutierrez Salvador Barahona Jennifer Alcaino Víctor Cifuentes |
author_facet |
Marcelo Baeza Sergio Zúñiga Vicente Peragallo Fernando Gutierrez Salvador Barahona Jennifer Alcaino Víctor Cifuentes |
author_sort |
Marcelo Baeza |
title |
Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX |
title_short |
Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX |
title_full |
Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX |
title_fullStr |
Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX |
title_full_unstemmed |
Table_1_Response to Cold: A Comparative Transcriptomic Analysis in Eight Cold-Adapted Yeasts.XLSX |
title_sort |
table_1_response to cold: a comparative transcriptomic analysis in eight cold-adapted yeasts.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.828536.s008 https://figshare.com/articles/dataset/Table_1_Response_to_Cold_A_Comparative_Transcriptomic_Analysis_in_Eight_Cold-Adapted_Yeasts_XLSX/19218165 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic King George Island |
genre_facet |
Antarc* Antarctic King George Island |
op_relation |
doi:10.3389/fmicb.2022.828536.s008 https://figshare.com/articles/dataset/Table_1_Response_to_Cold_A_Comparative_Transcriptomic_Analysis_in_Eight_Cold-Adapted_Yeasts_XLSX/19218165 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.828536.s008 |
_version_ |
1766273162162470912 |