Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG
The gut microbiome and the gut brain axis are potential determinants of Alzheimer’s disease (AD) etiology or severity and gut microbiota might coordinate with the gut-brain axis to regulate behavioral phenotypes in AD mouse models. Using 6-month-old human amyloid precursor protein (hAPP) knock-in (K...
Main Authors: | , , , , , , |
---|---|
Format: | Still Image |
Language: | unknown |
Published: |
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fnbeh.2022.791128.s001 https://figshare.com/articles/figure/Image_1_Fecal_Implants_From_AppNL_G_F_and_AppNL_G_F_E4_Donor_Mice_Sufficient_to_Induce_Behavioral_Phenotypes_in_Germ-Free_Mice_JPEG/19135013 |
id |
ftfrontimediafig:oai:figshare.com:article/19135013 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/19135013 2023-05-15T15:19:13+02:00 Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG Payel Kundu Keaton Stagaman Kristin Kasschau Sarah Holden Natalia Shulzhenko Thomas J. Sharpton Jacob Raber 2022-02-08T04:02:05Z https://doi.org/10.3389/fnbeh.2022.791128.s001 https://figshare.com/articles/figure/Image_1_Fecal_Implants_From_AppNL_G_F_and_AppNL_G_F_E4_Donor_Mice_Sufficient_to_Induce_Behavioral_Phenotypes_in_Germ-Free_Mice_JPEG/19135013 unknown doi:10.3389/fnbeh.2022.791128.s001 https://figshare.com/articles/figure/Image_1_Fecal_Implants_From_AppNL_G_F_and_AppNL_G_F_E4_Donor_Mice_Sufficient_to_Induce_Behavioral_Phenotypes_in_Germ-Free_Mice_JPEG/19135013 CC BY 4.0 CC-BY Behavioral Neuroscience Neuroscience Exercise Physiology Motor Control Central Nervous System Computer Perception Memory and Attention Decision Making germ-free mice fecal implants behavioral testing biosafety cabinet APP APOE gut microbiome cortical Aβ Image Figure 2022 ftfrontimediafig https://doi.org/10.3389/fnbeh.2022.791128.s001 2022-02-10T00:04:34Z The gut microbiome and the gut brain axis are potential determinants of Alzheimer’s disease (AD) etiology or severity and gut microbiota might coordinate with the gut-brain axis to regulate behavioral phenotypes in AD mouse models. Using 6-month-old human amyloid precursor protein (hAPP) knock-in (KI) mice, which contain the Swedish and Iberian mutations [APP NL-F (App NL–F )] or the Arctic mutation as third mutation [APP NL-G-F (App NL–G–F )], behavioral and cognitive performance is associated with the gut microbiome and APP genotype modulates this association. In this study, we determined the feasibility of behavioral testing of mice in a biosafety cabinet and whether stool from 6-month-old App NL–G–F mice or App NL–G–F crossed with human apoE4 targeted replacement mice is sufficient to induce behavioral phenotypes in 4-5 month-old germ-free C57BL/6J mice 4 weeks following inoculation. We also compared the behavioral phenotypes of the recipient mice with that of the donor mice. Finally, we assessed cortical Aβ levels and analyzed the gut microbiome in the recipient mice. These results show that it is feasible to behaviorally test germ-free mice inside a biosafety cabinet. However, the host genotype was critical in modulating the pattern of induced behavioral phenotypes as compared to those seen in the genotype- and sex-match donor mice. Male mice that received stool from App NL–G–F and App NL–G–F/E4 donor genotypes tended to have lower body weight as compared to wild type, an effect not observed among donor mice. Additionally, App NL–G–F/E4 recipient males, but not females, showed impaired object recognition. Insoluble Aβ40 levels were detected in App NL–G–F and App NL–G–F/E4 recipient mice. Recipients of App NL–G–F, but not App NL–G–F/E4 , donor mice carried cortical insoluble Aβ40 levels that positively correlated with activity levels on the first and second day of open field testing. For recipient mice, the interaction between donor genotype and several behavioral scores predicted gut microbiome ... Still Image Arctic Frontiers: Figshare Arctic |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Behavioral Neuroscience Neuroscience Exercise Physiology Motor Control Central Nervous System Computer Perception Memory and Attention Decision Making germ-free mice fecal implants behavioral testing biosafety cabinet APP APOE gut microbiome cortical Aβ |
spellingShingle |
Behavioral Neuroscience Neuroscience Exercise Physiology Motor Control Central Nervous System Computer Perception Memory and Attention Decision Making germ-free mice fecal implants behavioral testing biosafety cabinet APP APOE gut microbiome cortical Aβ Payel Kundu Keaton Stagaman Kristin Kasschau Sarah Holden Natalia Shulzhenko Thomas J. Sharpton Jacob Raber Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG |
topic_facet |
Behavioral Neuroscience Neuroscience Exercise Physiology Motor Control Central Nervous System Computer Perception Memory and Attention Decision Making germ-free mice fecal implants behavioral testing biosafety cabinet APP APOE gut microbiome cortical Aβ |
description |
The gut microbiome and the gut brain axis are potential determinants of Alzheimer’s disease (AD) etiology or severity and gut microbiota might coordinate with the gut-brain axis to regulate behavioral phenotypes in AD mouse models. Using 6-month-old human amyloid precursor protein (hAPP) knock-in (KI) mice, which contain the Swedish and Iberian mutations [APP NL-F (App NL–F )] or the Arctic mutation as third mutation [APP NL-G-F (App NL–G–F )], behavioral and cognitive performance is associated with the gut microbiome and APP genotype modulates this association. In this study, we determined the feasibility of behavioral testing of mice in a biosafety cabinet and whether stool from 6-month-old App NL–G–F mice or App NL–G–F crossed with human apoE4 targeted replacement mice is sufficient to induce behavioral phenotypes in 4-5 month-old germ-free C57BL/6J mice 4 weeks following inoculation. We also compared the behavioral phenotypes of the recipient mice with that of the donor mice. Finally, we assessed cortical Aβ levels and analyzed the gut microbiome in the recipient mice. These results show that it is feasible to behaviorally test germ-free mice inside a biosafety cabinet. However, the host genotype was critical in modulating the pattern of induced behavioral phenotypes as compared to those seen in the genotype- and sex-match donor mice. Male mice that received stool from App NL–G–F and App NL–G–F/E4 donor genotypes tended to have lower body weight as compared to wild type, an effect not observed among donor mice. Additionally, App NL–G–F/E4 recipient males, but not females, showed impaired object recognition. Insoluble Aβ40 levels were detected in App NL–G–F and App NL–G–F/E4 recipient mice. Recipients of App NL–G–F, but not App NL–G–F/E4 , donor mice carried cortical insoluble Aβ40 levels that positively correlated with activity levels on the first and second day of open field testing. For recipient mice, the interaction between donor genotype and several behavioral scores predicted gut microbiome ... |
format |
Still Image |
author |
Payel Kundu Keaton Stagaman Kristin Kasschau Sarah Holden Natalia Shulzhenko Thomas J. Sharpton Jacob Raber |
author_facet |
Payel Kundu Keaton Stagaman Kristin Kasschau Sarah Holden Natalia Shulzhenko Thomas J. Sharpton Jacob Raber |
author_sort |
Payel Kundu |
title |
Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG |
title_short |
Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG |
title_full |
Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG |
title_fullStr |
Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG |
title_full_unstemmed |
Image_1_Fecal Implants From AppNL–G–F and AppNL–G–F/E4 Donor Mice Sufficient to Induce Behavioral Phenotypes in Germ-Free Mice.JPEG |
title_sort |
image_1_fecal implants from appnl–g–f and appnl–g–f/e4 donor mice sufficient to induce behavioral phenotypes in germ-free mice.jpeg |
publishDate |
2022 |
url |
https://doi.org/10.3389/fnbeh.2022.791128.s001 https://figshare.com/articles/figure/Image_1_Fecal_Implants_From_AppNL_G_F_and_AppNL_G_F_E4_Donor_Mice_Sufficient_to_Induce_Behavioral_Phenotypes_in_Germ-Free_Mice_JPEG/19135013 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
doi:10.3389/fnbeh.2022.791128.s001 https://figshare.com/articles/figure/Image_1_Fecal_Implants_From_AppNL_G_F_and_AppNL_G_F_E4_Donor_Mice_Sufficient_to_Induce_Behavioral_Phenotypes_in_Germ-Free_Mice_JPEG/19135013 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fnbeh.2022.791128.s001 |
_version_ |
1766349402949025792 |