Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF
The active layer of permafrost in Ny Ålesund, Svalbard (79°N) around the Bayelva River in the Leirhaugen glacier moraine is measured as a small net carbon sink at the brink of becoming a carbon source. In many permafrost-dominating ecosystems, microbes in the active layers have been shown to drive o...
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Online Access: | https://doi.org/10.3389/fmicb.2021.757812.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Permafrost_Active_Layer_Microbes_From_Ny_lesund_Svalbard_79_N_Show_Autotrophic_and_Heterotrophic_Metabolisms_With_Diverse_Carbon-Degrading_Enzymes_PDF/19113773 |
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ftfrontimediafig:oai:figshare.com:article/19113773 2023-05-15T16:22:14+02:00 Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF Katie Sipes Raegan Paul Aubrey Fine Peibo Li Renxing Liang Julia Boike Tullis C. Onstott Tatiana A. Vishnivetskaya Sean Schaeffer Karen G. Lloyd 2022-02-03T05:13:36Z https://doi.org/10.3389/fmicb.2021.757812.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Permafrost_Active_Layer_Microbes_From_Ny_lesund_Svalbard_79_N_Show_Autotrophic_and_Heterotrophic_Metabolisms_With_Diverse_Carbon-Degrading_Enzymes_PDF/19113773 unknown doi:10.3389/fmicb.2021.757812.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Permafrost_Active_Layer_Microbes_From_Ny_lesund_Svalbard_79_N_Show_Autotrophic_and_Heterotrophic_Metabolisms_With_Diverse_Carbon-Degrading_Enzymes_PDF/19113773 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Svalbard permafrost active layer carbon nitrogen metagenome cultures enzymes Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2021.757812.s001 2022-02-10T00:07:39Z The active layer of permafrost in Ny Ålesund, Svalbard (79°N) around the Bayelva River in the Leirhaugen glacier moraine is measured as a small net carbon sink at the brink of becoming a carbon source. In many permafrost-dominating ecosystems, microbes in the active layers have been shown to drive organic matter degradation and greenhouse gas production, creating positive feedback on climate change. However, the microbial metabolisms linking the environmental geochemical processes and the populations that perform them have not been fully characterized. In this paper, we present geochemical, enzymatic, and isotopic data paired with 10 Pseudomonas sp. cultures and metagenomic libraries of two active layer soil cores (BPF1 and BPF2) from Ny Ålesund, Svalbard, (79°N). Relative to BPF1, BPF2 had statistically higher C/N ratios (15 ± 1 for BPF1 vs. 29 ± 10 for BPF2; n = 30, p < 10 –5 ), statistically lower organic carbon (2% ± 0.6% for BPF1 vs. 1.6% ± 0.4% for BPF2, p < 0.02), statistically lower nitrogen (0.1% ± 0.03% for BPF1 vs. 0.07% ± 0.02% for BPF2, p < 10 –6 ). The d 13 C values for inorganic carbon did not correlate with those of organic carbon in BPF2, suggesting lower heterotrophic respiration. An increase in the δ 13 C of inorganic carbon with depth either reflects an autotrophic signal or mixing between a heterotrophic source at the surface and a lithotrophic source at depth. Potential enzyme activity of xylosidase and N-acetyl-β-D-glucosaminidase increases twofold at 15°C, relative to 25°C, indicating cold adaptation in the cultures and bulk soil. Potential enzyme activity of leucine aminopeptidase across soils and cultures was two orders of magnitude higher than other tested enzymes, implying that organisms use leucine as a nitrogen and carbon source in this nutrient-limited environment. Besides demonstrating large variability in carbon compositions of permafrost active layer soils only ∼84 m apart, results suggest that the Svalbard active layer microbes are often limited by organic carbon or ... Dataset glacier Ny Ålesund Ny-Ålesund permafrost Svalbard Frontiers: Figshare Svalbard Ny-Ålesund Bayelva ENVELOPE(11.898,11.898,78.933,78.933) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Svalbard permafrost active layer carbon nitrogen metagenome cultures enzymes |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Svalbard permafrost active layer carbon nitrogen metagenome cultures enzymes Katie Sipes Raegan Paul Aubrey Fine Peibo Li Renxing Liang Julia Boike Tullis C. Onstott Tatiana A. Vishnivetskaya Sean Schaeffer Karen G. Lloyd Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Svalbard permafrost active layer carbon nitrogen metagenome cultures enzymes |
description |
The active layer of permafrost in Ny Ålesund, Svalbard (79°N) around the Bayelva River in the Leirhaugen glacier moraine is measured as a small net carbon sink at the brink of becoming a carbon source. In many permafrost-dominating ecosystems, microbes in the active layers have been shown to drive organic matter degradation and greenhouse gas production, creating positive feedback on climate change. However, the microbial metabolisms linking the environmental geochemical processes and the populations that perform them have not been fully characterized. In this paper, we present geochemical, enzymatic, and isotopic data paired with 10 Pseudomonas sp. cultures and metagenomic libraries of two active layer soil cores (BPF1 and BPF2) from Ny Ålesund, Svalbard, (79°N). Relative to BPF1, BPF2 had statistically higher C/N ratios (15 ± 1 for BPF1 vs. 29 ± 10 for BPF2; n = 30, p < 10 –5 ), statistically lower organic carbon (2% ± 0.6% for BPF1 vs. 1.6% ± 0.4% for BPF2, p < 0.02), statistically lower nitrogen (0.1% ± 0.03% for BPF1 vs. 0.07% ± 0.02% for BPF2, p < 10 –6 ). The d 13 C values for inorganic carbon did not correlate with those of organic carbon in BPF2, suggesting lower heterotrophic respiration. An increase in the δ 13 C of inorganic carbon with depth either reflects an autotrophic signal or mixing between a heterotrophic source at the surface and a lithotrophic source at depth. Potential enzyme activity of xylosidase and N-acetyl-β-D-glucosaminidase increases twofold at 15°C, relative to 25°C, indicating cold adaptation in the cultures and bulk soil. Potential enzyme activity of leucine aminopeptidase across soils and cultures was two orders of magnitude higher than other tested enzymes, implying that organisms use leucine as a nitrogen and carbon source in this nutrient-limited environment. Besides demonstrating large variability in carbon compositions of permafrost active layer soils only ∼84 m apart, results suggest that the Svalbard active layer microbes are often limited by organic carbon or ... |
format |
Dataset |
author |
Katie Sipes Raegan Paul Aubrey Fine Peibo Li Renxing Liang Julia Boike Tullis C. Onstott Tatiana A. Vishnivetskaya Sean Schaeffer Karen G. Lloyd |
author_facet |
Katie Sipes Raegan Paul Aubrey Fine Peibo Li Renxing Liang Julia Boike Tullis C. Onstott Tatiana A. Vishnivetskaya Sean Schaeffer Karen G. Lloyd |
author_sort |
Katie Sipes |
title |
Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF |
title_short |
Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF |
title_full |
Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF |
title_fullStr |
Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF |
title_full_unstemmed |
Data_Sheet_1_Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes.PDF |
title_sort |
data_sheet_1_permafrost active layer microbes from ny ålesund, svalbard (79°n) show autotrophic and heterotrophic metabolisms with diverse carbon-degrading enzymes.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2021.757812.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Permafrost_Active_Layer_Microbes_From_Ny_lesund_Svalbard_79_N_Show_Autotrophic_and_Heterotrophic_Metabolisms_With_Diverse_Carbon-Degrading_Enzymes_PDF/19113773 |
long_lat |
ENVELOPE(11.898,11.898,78.933,78.933) |
geographic |
Svalbard Ny-Ålesund Bayelva |
geographic_facet |
Svalbard Ny-Ålesund Bayelva |
genre |
glacier Ny Ålesund Ny-Ålesund permafrost Svalbard |
genre_facet |
glacier Ny Ålesund Ny-Ålesund permafrost Svalbard |
op_relation |
doi:10.3389/fmicb.2021.757812.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Permafrost_Active_Layer_Microbes_From_Ny_lesund_Svalbard_79_N_Show_Autotrophic_and_Heterotrophic_Metabolisms_With_Diverse_Carbon-Degrading_Enzymes_PDF/19113773 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.757812.s001 |
_version_ |
1766010205053648896 |