Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX
With the advent of the industrial revolution, the use of toxic compounds has grown exponentially, leading to a considerable pollution of the environment. Consequently, the development of more environmentally conscious technologies is an urgent need. Industrial biocatalysis appears as one potential s...
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ftfrontimediafig:oai:figshare.com:article/18233516 2023-05-15T13:43:30+02:00 Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX Giannina Espina Sebastián A. Muñoz-Ibacache Paulina Cáceres-Moreno Maximiliano J. Amenabar Jenny M. Blamey 2022-01-12T05:03:04Z https://doi.org/10.3389/fbioe.2021.752281.s001 https://figshare.com/articles/dataset/Table1_From_the_Discovery_of_Extremozymes_to_an_Enzymatic_Product_Roadmap_Based_on_Their_Applications_DOCX/18233516 unknown doi:10.3389/fbioe.2021.752281.s001 https://figshare.com/articles/dataset/Table1_From_the_Discovery_of_Extremozymes_to_an_Enzymatic_Product_Roadmap_Based_on_Their_Applications_DOCX/18233516 CC BY 4.0 CC-BY Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified biocatalysts extremophiles catalase laccase amine-transaminase Antarctica Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fbioe.2021.752281.s001 2022-01-13T00:01:23Z With the advent of the industrial revolution, the use of toxic compounds has grown exponentially, leading to a considerable pollution of the environment. Consequently, the development of more environmentally conscious technologies is an urgent need. Industrial biocatalysis appears as one potential solution, where a higher demand for more robust enzymes aims to replace toxic chemical catalysts. To date, most of the commercially available enzymes are of mesophilic origin, displaying optimal activity in narrow ranges of temperature and pH (i.e., between 20°C and 45°C, neutral pH), limiting their actual application under industrial reaction settings, where they usually underperform, requiring larger quantities to compensate loss of activity. In order to obtain novel biocatalysts better suited for industrial conditions, an efficient solution is to take advantage of nature by searching and discovering enzymes from extremophiles. These microorganisms and their macromolecules have already adapted to thrive in environments that present extreme physicochemical conditions. Hence, extremophilic enzymes stand out for showing higher activity, stability, and robustness than their mesophilic counterparts, being able to carry out reactions at nonstandard conditions. In this brief research report we describe three examples to illustrate a stepwise strategy for the development and production of commercial extremozymes, including a catalase from an Antarctic psychrotolerant microorganism, a laccase from a thermoalkaliphilic bacterium isolated from a hot spring and an amine-transaminase from a thermophilic bacterium isolated from a geothermal site in Antarctica. We will also explore some of their interesting biotechnological applications and comparisons with commercial enzymes. Dataset Antarc* Antarctic Antarctica Frontiers: Figshare Antarctic |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified biocatalysts extremophiles catalase laccase amine-transaminase Antarctica |
spellingShingle |
Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified biocatalysts extremophiles catalase laccase amine-transaminase Antarctica Giannina Espina Sebastián A. Muñoz-Ibacache Paulina Cáceres-Moreno Maximiliano J. Amenabar Jenny M. Blamey Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX |
topic_facet |
Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified biocatalysts extremophiles catalase laccase amine-transaminase Antarctica |
description |
With the advent of the industrial revolution, the use of toxic compounds has grown exponentially, leading to a considerable pollution of the environment. Consequently, the development of more environmentally conscious technologies is an urgent need. Industrial biocatalysis appears as one potential solution, where a higher demand for more robust enzymes aims to replace toxic chemical catalysts. To date, most of the commercially available enzymes are of mesophilic origin, displaying optimal activity in narrow ranges of temperature and pH (i.e., between 20°C and 45°C, neutral pH), limiting their actual application under industrial reaction settings, where they usually underperform, requiring larger quantities to compensate loss of activity. In order to obtain novel biocatalysts better suited for industrial conditions, an efficient solution is to take advantage of nature by searching and discovering enzymes from extremophiles. These microorganisms and their macromolecules have already adapted to thrive in environments that present extreme physicochemical conditions. Hence, extremophilic enzymes stand out for showing higher activity, stability, and robustness than their mesophilic counterparts, being able to carry out reactions at nonstandard conditions. In this brief research report we describe three examples to illustrate a stepwise strategy for the development and production of commercial extremozymes, including a catalase from an Antarctic psychrotolerant microorganism, a laccase from a thermoalkaliphilic bacterium isolated from a hot spring and an amine-transaminase from a thermophilic bacterium isolated from a geothermal site in Antarctica. We will also explore some of their interesting biotechnological applications and comparisons with commercial enzymes. |
format |
Dataset |
author |
Giannina Espina Sebastián A. Muñoz-Ibacache Paulina Cáceres-Moreno Maximiliano J. Amenabar Jenny M. Blamey |
author_facet |
Giannina Espina Sebastián A. Muñoz-Ibacache Paulina Cáceres-Moreno Maximiliano J. Amenabar Jenny M. Blamey |
author_sort |
Giannina Espina |
title |
Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX |
title_short |
Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX |
title_full |
Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX |
title_fullStr |
Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX |
title_full_unstemmed |
Table1_From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications.DOCX |
title_sort |
table1_from the discovery of extremozymes to an enzymatic product: roadmap based on their applications.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fbioe.2021.752281.s001 https://figshare.com/articles/dataset/Table1_From_the_Discovery_of_Extremozymes_to_an_Enzymatic_Product_Roadmap_Based_on_Their_Applications_DOCX/18233516 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.3389/fbioe.2021.752281.s001 https://figshare.com/articles/dataset/Table1_From_the_Discovery_of_Extremozymes_to_an_Enzymatic_Product_Roadmap_Based_on_Their_Applications_DOCX/18233516 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fbioe.2021.752281.s001 |
_version_ |
1766189783659315200 |