DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip
Salmonid rickettsial septicemia (SRS), caused by Piscirickettsia salmonis, is one of the most devastating diseases of salmonids. However, the transcriptomic responses of Atlantic salmon (Salmon salar) in freshwater to an EM-90-like isolate have not been explored. Here, we infected Atlantic salmon pa...
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2021
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Online Access: | https://doi.org/10.3389/fimmu.2021.789465.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Profiling_of_Atlantic_Salmon_Salmo_salar_Parr_With_Higher_and_Lower_Pathogen_Loads_Following_Piscirickettsia_salmonis_Infection_zip/17707616 |
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ftfrontimediafig:oai:figshare.com:article/17707616 2023-05-15T15:30:53+02:00 DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip Xi Xue Albert Caballero-Solares Jennifer R. Hall Navaneethaiyer Umasuthan Surendra Kumar Eva Jakob Stanko Skugor Christopher Hawes Javier Santander Richard G. Taylor Matthew L. Rise 2021-12-31T04:02:42Z https://doi.org/10.3389/fimmu.2021.789465.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Profiling_of_Atlantic_Salmon_Salmo_salar_Parr_With_Higher_and_Lower_Pathogen_Loads_Following_Piscirickettsia_salmonis_Infection_zip/17707616 unknown doi:10.3389/fimmu.2021.789465.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Profiling_of_Atlantic_Salmon_Salmo_salar_Parr_With_Higher_and_Lower_Pathogen_Loads_Following_Piscirickettsia_salmonis_Infection_zip/17707616 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmonid rickettsial septicaemia (SRS) EM-90 Salmo salar microarray immune response biomarkers freshwater disease resistance Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fimmu.2021.789465.s001 2022-01-06T00:05:43Z Salmonid rickettsial septicemia (SRS), caused by Piscirickettsia salmonis, is one of the most devastating diseases of salmonids. However, the transcriptomic responses of Atlantic salmon (Salmon salar) in freshwater to an EM-90-like isolate have not been explored. Here, we infected Atlantic salmon parr with an EM-90-like isolate and conducted time-course qPCR analyses of pathogen load and four biomarkers (campb, hampa, il8a, tlr5a) of innate immunity on the head kidney samples. Transcript expression of three of these genes (except hampa), as well as pathogen level, peaked at 21 days post-injection (DPI). Multivariate analyses of infected individuals at 21 DPI revealed two infection phenotypes [lower (L-SRS) and higher (H-SRS) infection level]. Five fish from each group (Control, L-SRS, and H-SRS) were selected for transcriptome profiling using a 44K salmonid microarray platform. We identified 1,636 and 3,076 differentially expressed probes (DEPs) in the L-SRS and H-SRS groups compared with the control group, respectively (FDR = 1%). Gene ontology term enrichment analyses of SRS-responsive genes revealed the activation of a large number of innate (e.g. “phagocytosis”, “defense response to bacterium”, “inflammatory response”) and adaptive (e.g. “regulation of T cell activation”, “antigen processing and presentation of exogenous antigen”) immune processes, while a small number of general physiological processes (e.g. “apoptotic process”, development and metabolism relevant) was enriched. Transcriptome results were confirmed by qPCR analyses of 42 microarray-identified transcripts. Furthermore, the comparison of individuals with differing levels of infection (H-SRS vs. L-SRS) generated insights into the biological processes possibly involved in disease resistance or susceptibility. This study demonstrated a low mortality (~30%) EM-90-like infection model and broadened the current understanding of molecular pathways underlying P. salmonis-triggered responses of Atlantic salmon, identifying biomarkers that may assist ... Dataset Atlantic salmon Salmo salar Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
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Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmonid rickettsial septicaemia (SRS) EM-90 Salmo salar microarray immune response biomarkers freshwater disease resistance |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmonid rickettsial septicaemia (SRS) EM-90 Salmo salar microarray immune response biomarkers freshwater disease resistance Xi Xue Albert Caballero-Solares Jennifer R. Hall Navaneethaiyer Umasuthan Surendra Kumar Eva Jakob Stanko Skugor Christopher Hawes Javier Santander Richard G. Taylor Matthew L. Rise DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Salmonid rickettsial septicaemia (SRS) EM-90 Salmo salar microarray immune response biomarkers freshwater disease resistance |
description |
Salmonid rickettsial septicemia (SRS), caused by Piscirickettsia salmonis, is one of the most devastating diseases of salmonids. However, the transcriptomic responses of Atlantic salmon (Salmon salar) in freshwater to an EM-90-like isolate have not been explored. Here, we infected Atlantic salmon parr with an EM-90-like isolate and conducted time-course qPCR analyses of pathogen load and four biomarkers (campb, hampa, il8a, tlr5a) of innate immunity on the head kidney samples. Transcript expression of three of these genes (except hampa), as well as pathogen level, peaked at 21 days post-injection (DPI). Multivariate analyses of infected individuals at 21 DPI revealed two infection phenotypes [lower (L-SRS) and higher (H-SRS) infection level]. Five fish from each group (Control, L-SRS, and H-SRS) were selected for transcriptome profiling using a 44K salmonid microarray platform. We identified 1,636 and 3,076 differentially expressed probes (DEPs) in the L-SRS and H-SRS groups compared with the control group, respectively (FDR = 1%). Gene ontology term enrichment analyses of SRS-responsive genes revealed the activation of a large number of innate (e.g. “phagocytosis”, “defense response to bacterium”, “inflammatory response”) and adaptive (e.g. “regulation of T cell activation”, “antigen processing and presentation of exogenous antigen”) immune processes, while a small number of general physiological processes (e.g. “apoptotic process”, development and metabolism relevant) was enriched. Transcriptome results were confirmed by qPCR analyses of 42 microarray-identified transcripts. Furthermore, the comparison of individuals with differing levels of infection (H-SRS vs. L-SRS) generated insights into the biological processes possibly involved in disease resistance or susceptibility. This study demonstrated a low mortality (~30%) EM-90-like infection model and broadened the current understanding of molecular pathways underlying P. salmonis-triggered responses of Atlantic salmon, identifying biomarkers that may assist ... |
format |
Dataset |
author |
Xi Xue Albert Caballero-Solares Jennifer R. Hall Navaneethaiyer Umasuthan Surendra Kumar Eva Jakob Stanko Skugor Christopher Hawes Javier Santander Richard G. Taylor Matthew L. Rise |
author_facet |
Xi Xue Albert Caballero-Solares Jennifer R. Hall Navaneethaiyer Umasuthan Surendra Kumar Eva Jakob Stanko Skugor Christopher Hawes Javier Santander Richard G. Taylor Matthew L. Rise |
author_sort |
Xi Xue |
title |
DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip |
title_short |
DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip |
title_full |
DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip |
title_fullStr |
DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip |
title_full_unstemmed |
DataSheet_1_Transcriptome Profiling of Atlantic Salmon (Salmo salar) Parr With Higher and Lower Pathogen Loads Following Piscirickettsia salmonis Infection.zip |
title_sort |
datasheet_1_transcriptome profiling of atlantic salmon (salmo salar) parr with higher and lower pathogen loads following piscirickettsia salmonis infection.zip |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.789465.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Profiling_of_Atlantic_Salmon_Salmo_salar_Parr_With_Higher_and_Lower_Pathogen_Loads_Following_Piscirickettsia_salmonis_Infection_zip/17707616 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fimmu.2021.789465.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Profiling_of_Atlantic_Salmon_Salmo_salar_Parr_With_Higher_and_Lower_Pathogen_Loads_Following_Piscirickettsia_salmonis_Infection_zip/17707616 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.789465.s001 |
_version_ |
1766361351043678208 |