Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls
Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a...
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ftfrontimediafig:oai:figshare.com:article/17206913 2023-05-15T13:58:46+02:00 Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls Ying Wang Huamin Wang Linghong Hu Liangbiao Chen 2021-12-15T14:22:47Z https://doi.org/10.3389/fphys.2021.740806.s003 https://figshare.com/articles/dataset/Table_3_Leptin_Gene_Protects_Against_Cold_Stress_in_Antarctic_Toothfish_xls/17206913 unknown doi:10.3389/fphys.2021.740806.s003 https://figshare.com/articles/dataset/Table_3_Leptin_Gene_Protects_Against_Cold_Stress_in_Antarctic_Toothfish_xls/17206913 CC BY 4.0 CC-BY Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fphys.2021.740806.s003 2021-12-15T23:59:56Z Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a gene (lepa) in the cold-adapted and neutrally buoyant Antarctic toothfish Dissostichus mawsoni that encodes a polypeptide carrying four α-helices and two cysteine residues forming in-chain disulfide bonds, structures shared by most vertebrate leptins. Quantitative RT-PCR confirmed that mRNA levels of the leptin-a gene of D. mawsoni (DM-lepa) were highest in muscle, followed by kidney and liver; detection levels were low in the gill, brain, intestine, and ovary tissues. Compared with leptin-a genes of fishes living in warmer waters, DM-lepa underwent rapid evolution and was subjected to positive selection. Over-expression of DM-lepa in the zebrafish cell line ZFL resulted in signal accumulation in the cytoplasm and significantly increased cell proliferation both at the normal culture temperature and under cold treatment. DM-lepa over-expression also reduced apoptosis under low-temperature stress and activated the STAT3 signaling pathway, in turn upregulating the anti-apoptotic proteins bcl2l1, bcl2a, myca and mdm2 while downregulating the pro-apoptotic baxa, p53 and caspase-3. These results demonstrate that DM-lepa, through STAT3 signaling, plays a protective role in cold stress by preventing apoptotic damage. Our study reveals a new role of lepa in polar fish. Dataset Antarc* Antarctic Antarctic Toothfish Frontiers: Figshare Antarctic |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni |
spellingShingle |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni Ying Wang Huamin Wang Linghong Hu Liangbiao Chen Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls |
topic_facet |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified leptin-a positive selection polar fish STAT3 signaling p53 Dissostichus mawsoni |
description |
Leptin is a cytokine-like peptide, predominantly biosynthesized in adipose tissue, which plays an important role in regulating food intake, energy balance and reproduction in mammals. However, how it may have been modified to enable life in the chronic cold is unclear. Here, we identified a leptin-a gene (lepa) in the cold-adapted and neutrally buoyant Antarctic toothfish Dissostichus mawsoni that encodes a polypeptide carrying four α-helices and two cysteine residues forming in-chain disulfide bonds, structures shared by most vertebrate leptins. Quantitative RT-PCR confirmed that mRNA levels of the leptin-a gene of D. mawsoni (DM-lepa) were highest in muscle, followed by kidney and liver; detection levels were low in the gill, brain, intestine, and ovary tissues. Compared with leptin-a genes of fishes living in warmer waters, DM-lepa underwent rapid evolution and was subjected to positive selection. Over-expression of DM-lepa in the zebrafish cell line ZFL resulted in signal accumulation in the cytoplasm and significantly increased cell proliferation both at the normal culture temperature and under cold treatment. DM-lepa over-expression also reduced apoptosis under low-temperature stress and activated the STAT3 signaling pathway, in turn upregulating the anti-apoptotic proteins bcl2l1, bcl2a, myca and mdm2 while downregulating the pro-apoptotic baxa, p53 and caspase-3. These results demonstrate that DM-lepa, through STAT3 signaling, plays a protective role in cold stress by preventing apoptotic damage. Our study reveals a new role of lepa in polar fish. |
format |
Dataset |
author |
Ying Wang Huamin Wang Linghong Hu Liangbiao Chen |
author_facet |
Ying Wang Huamin Wang Linghong Hu Liangbiao Chen |
author_sort |
Ying Wang |
title |
Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls |
title_short |
Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls |
title_full |
Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls |
title_fullStr |
Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls |
title_full_unstemmed |
Table_3_Leptin Gene Protects Against Cold Stress in Antarctic Toothfish.xls |
title_sort |
table_3_leptin gene protects against cold stress in antarctic toothfish.xls |
publishDate |
2021 |
url |
https://doi.org/10.3389/fphys.2021.740806.s003 https://figshare.com/articles/dataset/Table_3_Leptin_Gene_Protects_Against_Cold_Stress_in_Antarctic_Toothfish_xls/17206913 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Toothfish |
genre_facet |
Antarc* Antarctic Antarctic Toothfish |
op_relation |
doi:10.3389/fphys.2021.740806.s003 https://figshare.com/articles/dataset/Table_3_Leptin_Gene_Protects_Against_Cold_Stress_in_Antarctic_Toothfish_xls/17206913 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2021.740806.s003 |
_version_ |
1766267113355345920 |