Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential. Two such microbial “dark matter” taxa are the Candidatus bacterial phyla Cloacimonadota and Omnitrophota, both of which have been...
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ftfrontimediafig:oai:figshare.com:article/16782073 2023-05-15T14:02:18+02:00 Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX Timothy J. Williams Michelle A. Allen Jonathan F. Berengut Ricardo Cavicchioli 2021-10-11T04:53:47Z https://doi.org/10.3389/fmicb.2021.741077.s005 https://figshare.com/articles/dataset/Table_5_Shedding_Light_on_Microbial_Dark_Matter_Insights_Into_Novel_Cloacimonadota_and_Omnitrophota_From_an_Antarctic_Lake_XLSX/16782073 unknown doi:10.3389/fmicb.2021.741077.s005 https://figshare.com/articles/dataset/Table_5_Shedding_Light_on_Microbial_Dark_Matter_Insights_Into_Novel_Cloacimonadota_and_Omnitrophota_From_an_Antarctic_Lake_XLSX/16782073 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fmicb.2021.741077.s005 2021-10-13T23:00:45Z The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential. Two such microbial “dark matter” taxa are the Candidatus bacterial phyla Cloacimonadota and Omnitrophota, both of which have been identified in global anoxic environments, including (but not limited to) organic-carbon-rich lakes. Using 24 metagenome-assembled genomes (MAGs) obtained from an Antarctic lake (Ace Lake, Vestfold Hills), novel lineages and novel metabolic traits were identified for both phyla. The Cloacimonadota MAGs exhibited a capacity for carbon fixation using the reverse tricarboxylic acid cycle driven by oxidation of hydrogen and sulfur. Certain Cloacimonadota MAGs encoded proteins that possess dockerin and cohesin domains, which is consistent with the assembly of extracellular cellulosome-like structures that are used for degradation of polypeptides and polysaccharides. The Omnitrophota MAGs represented phylogenetically diverse taxa that were predicted to possess a strong biosynthetic capacity for amino acids, nucleosides, fatty acids, and essential cofactors. All of the Omnitrophota were inferred to be obligate fermentative heterotrophs that utilize a relatively narrow range of organic compounds, have an incomplete tricarboxylic acid cycle, and possess a single hydrogenase gene important for achieving redox balance in the cell. We reason that both Cloacimonadota and Omnitrophota form metabolic interactions with hydrogen-consuming partners (methanogens and Desulfobacterota, respectively) and, therefore, occupy specific niches in Ace Lake. Dataset Antarc* Antarctic Frontiers: Figshare Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Antarctic Vestfold Vestfold Hills |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria Timothy J. Williams Michelle A. Allen Jonathan F. Berengut Ricardo Cavicchioli Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Cloacimonadota Omnitrophota cellulosome autotrophy metagenome Antarctic bacteria |
description |
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential. Two such microbial “dark matter” taxa are the Candidatus bacterial phyla Cloacimonadota and Omnitrophota, both of which have been identified in global anoxic environments, including (but not limited to) organic-carbon-rich lakes. Using 24 metagenome-assembled genomes (MAGs) obtained from an Antarctic lake (Ace Lake, Vestfold Hills), novel lineages and novel metabolic traits were identified for both phyla. The Cloacimonadota MAGs exhibited a capacity for carbon fixation using the reverse tricarboxylic acid cycle driven by oxidation of hydrogen and sulfur. Certain Cloacimonadota MAGs encoded proteins that possess dockerin and cohesin domains, which is consistent with the assembly of extracellular cellulosome-like structures that are used for degradation of polypeptides and polysaccharides. The Omnitrophota MAGs represented phylogenetically diverse taxa that were predicted to possess a strong biosynthetic capacity for amino acids, nucleosides, fatty acids, and essential cofactors. All of the Omnitrophota were inferred to be obligate fermentative heterotrophs that utilize a relatively narrow range of organic compounds, have an incomplete tricarboxylic acid cycle, and possess a single hydrogenase gene important for achieving redox balance in the cell. We reason that both Cloacimonadota and Omnitrophota form metabolic interactions with hydrogen-consuming partners (methanogens and Desulfobacterota, respectively) and, therefore, occupy specific niches in Ace Lake. |
format |
Dataset |
author |
Timothy J. Williams Michelle A. Allen Jonathan F. Berengut Ricardo Cavicchioli |
author_facet |
Timothy J. Williams Michelle A. Allen Jonathan F. Berengut Ricardo Cavicchioli |
author_sort |
Timothy J. Williams |
title |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_short |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_full |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_fullStr |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_full_unstemmed |
Table_5_Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake.XLSX |
title_sort |
table_5_shedding light on microbial “dark matter”: insights into novel cloacimonadota and omnitrophota from an antarctic lake.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.741077.s005 https://figshare.com/articles/dataset/Table_5_Shedding_Light_on_Microbial_Dark_Matter_Insights_Into_Novel_Cloacimonadota_and_Omnitrophota_From_an_Antarctic_Lake_XLSX/16782073 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake Antarctic Vestfold Vestfold Hills |
geographic_facet |
Ace Lake Antarctic Vestfold Vestfold Hills |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.3389/fmicb.2021.741077.s005 https://figshare.com/articles/dataset/Table_5_Shedding_Light_on_Microbial_Dark_Matter_Insights_Into_Novel_Cloacimonadota_and_Omnitrophota_From_an_Antarctic_Lake_XLSX/16782073 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.741077.s005 |
_version_ |
1766272520126726144 |