Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf
The presence and diversity of marine non-native species, the number of new invasions, and the impact on native communities and habitats are important metrics used to assess the health of marine ecosystems. Monitoring for marine non-native species, using traditional approaches such as rapid assessmen...
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ftfrontimediafig:oai:figshare.com:article/16591082 2023-05-15T17:54:22+02:00 Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf Iveta Matejusova Jennifer Graham Fiona Bland Jean-Pierre Lacaze Guillaume Herman Lyndsay Brown Eric Dalgarno John D. Bishop Jenni E. Kakkonen Kirsty F. Smith Alex Douglas 2021-09-09T04:06:40Z https://doi.org/10.3389/fmars.2021.728456.s001 https://figshare.com/articles/figure/Image_1_Environmental_DNA_Based_Surveillance_for_the_Highly_Invasive_Carpet_Sea_Squirt_Didemnum_vexillum_A_Targeted_Single-Species_Approach_pdf/16591082 unknown doi:10.3389/fmars.2021.728456.s001 https://figshare.com/articles/figure/Image_1_Environmental_DNA_Based_Surveillance_for_the_Highly_Invasive_Carpet_Sea_Squirt_Didemnum_vexillum_A_Targeted_Single-Species_Approach_pdf/16591082 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering eDNA invasive species qPCR aquaculture monitoring marina Didemnum vexillum Image Figure 2021 ftfrontimediafig https://doi.org/10.3389/fmars.2021.728456.s001 2021-09-15T23:03:08Z The presence and diversity of marine non-native species, the number of new invasions, and the impact on native communities and habitats are important metrics used to assess the health of marine ecosystems. Monitoring for marine non-native species, using traditional approaches such as rapid assessment surveys (RASs), requires taxonomic expertise and may still fail to detect rare or inconspicuous species. This study reports a validation process for a quantitative PCR (qPCR) assay based on the cytochrome oxidase 1 gene, designed to detect highly invasive tunicate Didemnum vexillum by targeting environmental DNA (eDNA) present in water samples. The D. vexillum qPCR assay showed high sensitivity, with the threshold limit of detection (LOD) and modeled LOD3 (based on triplicate qPCR reactions) estimated as 9.187 and 1.117 copies reaction –1 , respectively and the limit of quantification (LOQ) was calculated as 18 copies reaction –1 . Analyses of water samples collected from selected Pacific oyster farms and recreational marinas in Scotland showed 100% concordance between the historical data on presence of D. vexillum from RASs and detection of D. vexillum eDNA. Consistency of detection of D. vexillum eDNA among different sampling points within each infected sampling site varied, ranging between 100% positive throughout the site to some sampling points testing “negative” or only as “suspected” for D. vexillum. Sites with lower within-site detection consistency included sites with a low density of D. vexillum as reported by RASs or were sites undergoing D. vexillum management. The present pilot monitoring program demonstrates the potential to generate important data on presence of D. vexillum. This program will be scaled up across large geographic regions and used in the first instance to focus and target the traditional RASs to D. vexillum eDNA-positive sites in a cost-effective way, with an aim to verify the species presence by visual observation and direct Sanger sequencing of positive qPCR products. Still Image Pacific oyster Frontiers: Figshare Pacific |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering eDNA invasive species qPCR aquaculture monitoring marina Didemnum vexillum |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering eDNA invasive species qPCR aquaculture monitoring marina Didemnum vexillum Iveta Matejusova Jennifer Graham Fiona Bland Jean-Pierre Lacaze Guillaume Herman Lyndsay Brown Eric Dalgarno John D. Bishop Jenni E. Kakkonen Kirsty F. Smith Alex Douglas Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering eDNA invasive species qPCR aquaculture monitoring marina Didemnum vexillum |
description |
The presence and diversity of marine non-native species, the number of new invasions, and the impact on native communities and habitats are important metrics used to assess the health of marine ecosystems. Monitoring for marine non-native species, using traditional approaches such as rapid assessment surveys (RASs), requires taxonomic expertise and may still fail to detect rare or inconspicuous species. This study reports a validation process for a quantitative PCR (qPCR) assay based on the cytochrome oxidase 1 gene, designed to detect highly invasive tunicate Didemnum vexillum by targeting environmental DNA (eDNA) present in water samples. The D. vexillum qPCR assay showed high sensitivity, with the threshold limit of detection (LOD) and modeled LOD3 (based on triplicate qPCR reactions) estimated as 9.187 and 1.117 copies reaction –1 , respectively and the limit of quantification (LOQ) was calculated as 18 copies reaction –1 . Analyses of water samples collected from selected Pacific oyster farms and recreational marinas in Scotland showed 100% concordance between the historical data on presence of D. vexillum from RASs and detection of D. vexillum eDNA. Consistency of detection of D. vexillum eDNA among different sampling points within each infected sampling site varied, ranging between 100% positive throughout the site to some sampling points testing “negative” or only as “suspected” for D. vexillum. Sites with lower within-site detection consistency included sites with a low density of D. vexillum as reported by RASs or were sites undergoing D. vexillum management. The present pilot monitoring program demonstrates the potential to generate important data on presence of D. vexillum. This program will be scaled up across large geographic regions and used in the first instance to focus and target the traditional RASs to D. vexillum eDNA-positive sites in a cost-effective way, with an aim to verify the species presence by visual observation and direct Sanger sequencing of positive qPCR products. |
format |
Still Image |
author |
Iveta Matejusova Jennifer Graham Fiona Bland Jean-Pierre Lacaze Guillaume Herman Lyndsay Brown Eric Dalgarno John D. Bishop Jenni E. Kakkonen Kirsty F. Smith Alex Douglas |
author_facet |
Iveta Matejusova Jennifer Graham Fiona Bland Jean-Pierre Lacaze Guillaume Herman Lyndsay Brown Eric Dalgarno John D. Bishop Jenni E. Kakkonen Kirsty F. Smith Alex Douglas |
author_sort |
Iveta Matejusova |
title |
Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf |
title_short |
Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf |
title_full |
Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf |
title_fullStr |
Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf |
title_full_unstemmed |
Image_1_Environmental DNA Based Surveillance for the Highly Invasive Carpet Sea Squirt Didemnum vexillum: A Targeted Single-Species Approach.pdf |
title_sort |
image_1_environmental dna based surveillance for the highly invasive carpet sea squirt didemnum vexillum: a targeted single-species approach.pdf |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmars.2021.728456.s001 https://figshare.com/articles/figure/Image_1_Environmental_DNA_Based_Surveillance_for_the_Highly_Invasive_Carpet_Sea_Squirt_Didemnum_vexillum_A_Targeted_Single-Species_Approach_pdf/16591082 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
doi:10.3389/fmars.2021.728456.s001 https://figshare.com/articles/figure/Image_1_Environmental_DNA_Based_Surveillance_for_the_Highly_Invasive_Carpet_Sea_Squirt_Didemnum_vexillum_A_Targeted_Single-Species_Approach_pdf/16591082 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2021.728456.s001 |
_version_ |
1766162119673249792 |