Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx
The Pacific oyster Crassostrea gigas is the world’s most cultivated oyster and seed supply is heavily reliant on hatchery production where recurring mass mortality events are a major constraint. Outbreaks of bacterial infection via microalgal feed are frequently implicated in these mortalities. This...
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Online Access: | https://doi.org/10.3389/fmicb.2021.706214.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Dietary_Exposure_of_Pacific_Oyster_Crassostrea_gigas_Larvae_to_Compromised_Microalgae_Results_in_Impaired_Fitness_and_Microbiome_Shift_docx/16418274 |
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ftfrontimediafig:oai:figshare.com:article/16418274 2023-05-15T15:57:54+02:00 Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Nick King 2021-08-24T05:06:57Z https://doi.org/10.3389/fmicb.2021.706214.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Dietary_Exposure_of_Pacific_Oyster_Crassostrea_gigas_Larvae_to_Compromised_Microalgae_Results_in_Impaired_Fitness_and_Microbiome_Shift_docx/16418274 unknown doi:10.3389/fmicb.2021.706214.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Dietary_Exposure_of_Pacific_Oyster_Crassostrea_gigas_Larvae_to_Compromised_Microalgae_Results_in_Impaired_Fitness_and_Microbiome_Shift_docx/16418274 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology oyster hatchery larvae Crassostrea gigas microalgae fitness microbiome 16S rRNA gene sequencing Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fmicb.2021.706214.s001 2021-08-25T22:58:57Z The Pacific oyster Crassostrea gigas is the world’s most cultivated oyster and seed supply is heavily reliant on hatchery production where recurring mass mortality events are a major constraint. Outbreaks of bacterial infection via microalgal feed are frequently implicated in these mortalities. This study assessed the effects of feeding compromised microalgae to developing oyster larvae. Intentionally ‘stressed’ (high pH) or non-stressed microalgae were fed to 11 day-old oyster larvae at two feeding rations for 96 h, followed by a recovery period. Biological endpoints of larval performance were measured following the 96 h exposure and subsequent recovery. Bacterial communities associated with the microalgae feed, rearing seawater, and the oyster larvae, were characterized and correlated with effects on oyster fitness parameters. Feeding stressed algae to oyster larvae for 96 h increased the occurrence of deformities (>70% vs. 20% in control), reduced feeding and swimming ability, and slowed development. Following the recovery period, fewer larvae reached pediveliger stage (2.7% vs. 36% in control) and became spat (1.5% vs. 6.6% in control). The quantity of stressed algae supplied to oyster larvae also influenced overall larval performance, with high feeding rations generally causing greater impairment than low rations. Bacterial profiling using 16S rRNA showed that most bacterial families characterized in larval tissue were also present in larval rearing seawater and in the microalgae feed (98%). The rearing seawater showed the highest bacterial richness compared to the larval and the microalgal compartments, regardless of feeding regime. In larval tissue, bacterial richness was highest in stressed and high-feed treatments, and negatively correlated with larval fitness parameters. These results suggest significant dysbiosis induced by compromised feed and/or increased feed ration. Several bacterial genera (e.g., Halomonas, Marinomonas) were strongly associated with impaired larval performance while the ... Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology oyster hatchery larvae Crassostrea gigas microalgae fitness microbiome 16S rRNA gene sequencing |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology oyster hatchery larvae Crassostrea gigas microalgae fitness microbiome 16S rRNA gene sequencing Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Nick King Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology oyster hatchery larvae Crassostrea gigas microalgae fitness microbiome 16S rRNA gene sequencing |
description |
The Pacific oyster Crassostrea gigas is the world’s most cultivated oyster and seed supply is heavily reliant on hatchery production where recurring mass mortality events are a major constraint. Outbreaks of bacterial infection via microalgal feed are frequently implicated in these mortalities. This study assessed the effects of feeding compromised microalgae to developing oyster larvae. Intentionally ‘stressed’ (high pH) or non-stressed microalgae were fed to 11 day-old oyster larvae at two feeding rations for 96 h, followed by a recovery period. Biological endpoints of larval performance were measured following the 96 h exposure and subsequent recovery. Bacterial communities associated with the microalgae feed, rearing seawater, and the oyster larvae, were characterized and correlated with effects on oyster fitness parameters. Feeding stressed algae to oyster larvae for 96 h increased the occurrence of deformities (>70% vs. 20% in control), reduced feeding and swimming ability, and slowed development. Following the recovery period, fewer larvae reached pediveliger stage (2.7% vs. 36% in control) and became spat (1.5% vs. 6.6% in control). The quantity of stressed algae supplied to oyster larvae also influenced overall larval performance, with high feeding rations generally causing greater impairment than low rations. Bacterial profiling using 16S rRNA showed that most bacterial families characterized in larval tissue were also present in larval rearing seawater and in the microalgae feed (98%). The rearing seawater showed the highest bacterial richness compared to the larval and the microalgal compartments, regardless of feeding regime. In larval tissue, bacterial richness was highest in stressed and high-feed treatments, and negatively correlated with larval fitness parameters. These results suggest significant dysbiosis induced by compromised feed and/or increased feed ration. Several bacterial genera (e.g., Halomonas, Marinomonas) were strongly associated with impaired larval performance while the ... |
format |
Dataset |
author |
Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Nick King |
author_facet |
Julien Vignier Olivier Laroche Anne Rolton Pandora Wadsworth Karthiga Kumanan Branwen Trochel Xavier Pochon Nick King |
author_sort |
Julien Vignier |
title |
Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx |
title_short |
Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx |
title_full |
Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx |
title_fullStr |
Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx |
title_full_unstemmed |
Data_Sheet_1_Dietary Exposure of Pacific Oyster (Crassostrea gigas) Larvae to Compromised Microalgae Results in Impaired Fitness and Microbiome Shift.docx |
title_sort |
data_sheet_1_dietary exposure of pacific oyster (crassostrea gigas) larvae to compromised microalgae results in impaired fitness and microbiome shift.docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.706214.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Dietary_Exposure_of_Pacific_Oyster_Crassostrea_gigas_Larvae_to_Compromised_Microalgae_Results_in_Impaired_Fitness_and_Microbiome_Shift_docx/16418274 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fmicb.2021.706214.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Dietary_Exposure_of_Pacific_Oyster_Crassostrea_gigas_Larvae_to_Compromised_Microalgae_Results_in_Impaired_Fitness_and_Microbiome_Shift_docx/16418274 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.706214.s001 |
_version_ |
1766393612145262592 |