Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx
The Atlantic salmon (Salmo salar) is an economically important fish, both in aquaculture and in the wild. In vertebrates, macrophages are some of the first cell types to respond to pathogen infection and disease. While macrophage biology has been characterized in mammals, less is known in fish. Our...
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ftfrontimediafig:oai:figshare.com:article/15171777 2023-05-15T15:29:57+02:00 Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx Nicole C. Smith Navaneethaiyer Umasuthan Surendra Kumar Nardos T. Woldemariam Rune Andreassen Sherri L. Christian Matthew L. Rise 2021-08-16T05:03:03Z https://doi.org/10.3389/fimmu.2021.709910.s007 https://figshare.com/articles/dataset/Table_7_Transcriptome_Profiling_of_Atlantic_Salmon_Adherent_Head_Kidney_Leukocytes_Reveals_That_Macrophages_Are_Selectively_Enriched_During_Culture_xlsx/15171777 unknown doi:10.3389/fimmu.2021.709910.s007 https://figshare.com/articles/dataset/Table_7_Transcriptome_Profiling_of_Atlantic_Salmon_Adherent_Head_Kidney_Leukocytes_Reveals_That_Macrophages_Are_Selectively_Enriched_During_Culture_xlsx/15171777 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon transcriptome macrophage microarray cell differentiation head kidney leukocyte culture Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fimmu.2021.709910.s007 2021-08-18T23:00:18Z The Atlantic salmon (Salmo salar) is an economically important fish, both in aquaculture and in the wild. In vertebrates, macrophages are some of the first cell types to respond to pathogen infection and disease. While macrophage biology has been characterized in mammals, less is known in fish. Our previous work identified changes in the morphology, phagocytic ability, and miRNA profile of Atlantic salmon adherent head kidney leukocytes (HKLs) from predominantly “monocyte-like” at Day 1 of in vitro culture to predominantly “macrophage-like” at Day 5 of culture. Therefore, to further characterize these two cell populations, we examined the mRNA transcriptome profile in Day 1 and Day 5 HKLs using a 44K oligonucleotide microarray. Large changes in the transcriptome were revealed, including changes in the expression of macrophage and immune-related transcripts (e.g. csf1r, arg1, tnfa, mx2), lipid-related transcripts (e.g. fasn, dhcr7, fabp6), and transcription factors involved in macrophage differentiation and function (e.g. klf2, klf9, irf7, irf8, stat1). The in silico target prediction analysis of differentially expressed genes (DEGs) using miRNAs known to change expression in Day 5 HKLs, followed by gene pathway enrichment analysis, supported that these miRNAs may be involved in macrophage maturation by targeting specific DEGs. Elucidating how immune cells, such as macrophages, develop and function is a key step in understanding the Atlantic salmon immune system. Overall, the results indicate that, without the addition of exogenous factors, the adherent HKL cell population differentiates in vitro to become macrophage-like. Dataset Atlantic salmon Salmo salar Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon transcriptome macrophage microarray cell differentiation head kidney leukocyte culture |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon transcriptome macrophage microarray cell differentiation head kidney leukocyte culture Nicole C. Smith Navaneethaiyer Umasuthan Surendra Kumar Nardos T. Woldemariam Rune Andreassen Sherri L. Christian Matthew L. Rise Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon transcriptome macrophage microarray cell differentiation head kidney leukocyte culture |
description |
The Atlantic salmon (Salmo salar) is an economically important fish, both in aquaculture and in the wild. In vertebrates, macrophages are some of the first cell types to respond to pathogen infection and disease. While macrophage biology has been characterized in mammals, less is known in fish. Our previous work identified changes in the morphology, phagocytic ability, and miRNA profile of Atlantic salmon adherent head kidney leukocytes (HKLs) from predominantly “monocyte-like” at Day 1 of in vitro culture to predominantly “macrophage-like” at Day 5 of culture. Therefore, to further characterize these two cell populations, we examined the mRNA transcriptome profile in Day 1 and Day 5 HKLs using a 44K oligonucleotide microarray. Large changes in the transcriptome were revealed, including changes in the expression of macrophage and immune-related transcripts (e.g. csf1r, arg1, tnfa, mx2), lipid-related transcripts (e.g. fasn, dhcr7, fabp6), and transcription factors involved in macrophage differentiation and function (e.g. klf2, klf9, irf7, irf8, stat1). The in silico target prediction analysis of differentially expressed genes (DEGs) using miRNAs known to change expression in Day 5 HKLs, followed by gene pathway enrichment analysis, supported that these miRNAs may be involved in macrophage maturation by targeting specific DEGs. Elucidating how immune cells, such as macrophages, develop and function is a key step in understanding the Atlantic salmon immune system. Overall, the results indicate that, without the addition of exogenous factors, the adherent HKL cell population differentiates in vitro to become macrophage-like. |
format |
Dataset |
author |
Nicole C. Smith Navaneethaiyer Umasuthan Surendra Kumar Nardos T. Woldemariam Rune Andreassen Sherri L. Christian Matthew L. Rise |
author_facet |
Nicole C. Smith Navaneethaiyer Umasuthan Surendra Kumar Nardos T. Woldemariam Rune Andreassen Sherri L. Christian Matthew L. Rise |
author_sort |
Nicole C. Smith |
title |
Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx |
title_short |
Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx |
title_full |
Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx |
title_fullStr |
Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx |
title_full_unstemmed |
Table_7_Transcriptome Profiling of Atlantic Salmon Adherent Head Kidney Leukocytes Reveals That Macrophages Are Selectively Enriched During Culture.xlsx |
title_sort |
table_7_transcriptome profiling of atlantic salmon adherent head kidney leukocytes reveals that macrophages are selectively enriched during culture.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.709910.s007 https://figshare.com/articles/dataset/Table_7_Transcriptome_Profiling_of_Atlantic_Salmon_Adherent_Head_Kidney_Leukocytes_Reveals_That_Macrophages_Are_Selectively_Enriched_During_Culture_xlsx/15171777 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fimmu.2021.709910.s007 https://figshare.com/articles/dataset/Table_7_Transcriptome_Profiling_of_Atlantic_Salmon_Adherent_Head_Kidney_Leukocytes_Reveals_That_Macrophages_Are_Selectively_Enriched_During_Culture_xlsx/15171777 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.709910.s007 |
_version_ |
1766360403611222016 |