Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf
Arsenic contamination in water by natural causes or industrial activities is a major environmental concern, and treatment of contaminated waters is needed to protect water resources and minimize the risk for human health. In mining environments, treatment peatlands are used in the polishing phase of...
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Online Access: | https://doi.org/10.3389/fmicb.2021.648412.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Assessing_the_Diversity_and_Metabolic_Potential_of_Psychrotolerant_Arsenic-Metabolizing_Microorganisms_From_a_Subarctic_Peatland_Used_for_Treatment_of_Mining-Affected_Waters_by_Culture-Dependent_and_-Independent_Techniques_pdf/14915625 |
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ftfrontimediafig:oai:figshare.com:article/14915625 2023-05-15T18:28:38+02:00 Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf Aileen Ziegelhöfer Katharina Kujala 2021-07-06T11:39:27Z https://doi.org/10.3389/fmicb.2021.648412.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Assessing_the_Diversity_and_Metabolic_Potential_of_Psychrotolerant_Arsenic-Metabolizing_Microorganisms_From_a_Subarctic_Peatland_Used_for_Treatment_of_Mining-Affected_Waters_by_Culture-Dependent_and_-Independent_Techniques_pdf/14915625 unknown doi:10.3389/fmicb.2021.648412.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Assessing_the_Diversity_and_Metabolic_Potential_of_Psychrotolerant_Arsenic-Metabolizing_Microorganisms_From_a_Subarctic_Peatland_Used_for_Treatment_of_Mining-Affected_Waters_by_Culture-Dependent_and_-Independent_Techniques_pdf/14915625 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology peatland mining wastewater treatment psychrotolerant microorganisms arsenic metabolizing microbes dilution to extinction isolation Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fmicb.2021.648412.s001 2021-07-07T22:58:04Z Arsenic contamination in water by natural causes or industrial activities is a major environmental concern, and treatment of contaminated waters is needed to protect water resources and minimize the risk for human health. In mining environments, treatment peatlands are used in the polishing phase of water treatment to remove arsenic (among other contaminants), and peat microorganisms play a crucial role in arsenic removal. The present study assessed culture-independent diversity obtained through metagenomic and metatranscriptomic sequencing and culture-dependent diversity obtained by isolating psychrotolerant arsenic-tolerant, arsenite-oxidizing, and arsenate-respiring microorganisms from a peatland treating mine effluent waters of a gold mine in Finnish Lapland using a dilution-to-extinction technique. Low diversity enrichments obtained after several transfers were dominated by the genera Pseudomonas, Polaromonas, Aeromonas, Brevundimonas, Ancylobacter, and Rhodoferax. Even though maximal growth and physiological activity (i.e., arsenite oxidation or arsenate reduction) were observed at temperatures between 20 and 28°C, most enrichments also showed substantial growth/activity at 2–5°C, indicating the successful enrichments of psychrotolerant microorganisms. After additional purification, eight arsenic-tolerant, five arsenite-oxidizing, and three arsenate-respiring strains were obtained in pure culture and identified as Pseudomonas, Rhodococcus, Microbacterium, and Cadophora. Some of the enriched and isolated genera are not known to metabolize arsenic, and valuable insights on arsenic turnover pathways may be gained by their further characterization. Comparison with phylogenetic and functional data from the metagenome indicated that the enriched and isolated strains did not belong to the most abundant genera, indicating that culture-dependent and -independent methods capture different fractions of the microbial community involved in arsenic turnover. Rare biosphere microorganisms that are present in low ... Dataset Subarctic Lapland Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology peatland mining wastewater treatment psychrotolerant microorganisms arsenic metabolizing microbes dilution to extinction isolation |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology peatland mining wastewater treatment psychrotolerant microorganisms arsenic metabolizing microbes dilution to extinction isolation Aileen Ziegelhöfer Katharina Kujala Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology peatland mining wastewater treatment psychrotolerant microorganisms arsenic metabolizing microbes dilution to extinction isolation |
description |
Arsenic contamination in water by natural causes or industrial activities is a major environmental concern, and treatment of contaminated waters is needed to protect water resources and minimize the risk for human health. In mining environments, treatment peatlands are used in the polishing phase of water treatment to remove arsenic (among other contaminants), and peat microorganisms play a crucial role in arsenic removal. The present study assessed culture-independent diversity obtained through metagenomic and metatranscriptomic sequencing and culture-dependent diversity obtained by isolating psychrotolerant arsenic-tolerant, arsenite-oxidizing, and arsenate-respiring microorganisms from a peatland treating mine effluent waters of a gold mine in Finnish Lapland using a dilution-to-extinction technique. Low diversity enrichments obtained after several transfers were dominated by the genera Pseudomonas, Polaromonas, Aeromonas, Brevundimonas, Ancylobacter, and Rhodoferax. Even though maximal growth and physiological activity (i.e., arsenite oxidation or arsenate reduction) were observed at temperatures between 20 and 28°C, most enrichments also showed substantial growth/activity at 2–5°C, indicating the successful enrichments of psychrotolerant microorganisms. After additional purification, eight arsenic-tolerant, five arsenite-oxidizing, and three arsenate-respiring strains were obtained in pure culture and identified as Pseudomonas, Rhodococcus, Microbacterium, and Cadophora. Some of the enriched and isolated genera are not known to metabolize arsenic, and valuable insights on arsenic turnover pathways may be gained by their further characterization. Comparison with phylogenetic and functional data from the metagenome indicated that the enriched and isolated strains did not belong to the most abundant genera, indicating that culture-dependent and -independent methods capture different fractions of the microbial community involved in arsenic turnover. Rare biosphere microorganisms that are present in low ... |
format |
Dataset |
author |
Aileen Ziegelhöfer Katharina Kujala |
author_facet |
Aileen Ziegelhöfer Katharina Kujala |
author_sort |
Aileen Ziegelhöfer |
title |
Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf |
title_short |
Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf |
title_full |
Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf |
title_fullStr |
Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf |
title_full_unstemmed |
Data_Sheet_1_Assessing the Diversity and Metabolic Potential of Psychrotolerant Arsenic-Metabolizing Microorganisms From a Subarctic Peatland Used for Treatment of Mining-Affected Waters by Culture-Dependent and -Independent Techniques.pdf |
title_sort |
data_sheet_1_assessing the diversity and metabolic potential of psychrotolerant arsenic-metabolizing microorganisms from a subarctic peatland used for treatment of mining-affected waters by culture-dependent and -independent techniques.pdf |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.648412.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Assessing_the_Diversity_and_Metabolic_Potential_of_Psychrotolerant_Arsenic-Metabolizing_Microorganisms_From_a_Subarctic_Peatland_Used_for_Treatment_of_Mining-Affected_Waters_by_Culture-Dependent_and_-Independent_Techniques_pdf/14915625 |
genre |
Subarctic Lapland |
genre_facet |
Subarctic Lapland |
op_relation |
doi:10.3389/fmicb.2021.648412.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Assessing_the_Diversity_and_Metabolic_Potential_of_Psychrotolerant_Arsenic-Metabolizing_Microorganisms_From_a_Subarctic_Peatland_Used_for_Treatment_of_Mining-Affected_Waters_by_Culture-Dependent_and_-Independent_Techniques_pdf/14915625 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.648412.s001 |
_version_ |
1766211178229399552 |