DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc
Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cy...
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Online Access: | https://doi.org/10.3389/fimmu.2021.689783.s001 https://figshare.com/articles/dataset/DataSheet_1_The_cGAS_STING_TBK1_IRF_Regulatory_Axis_Orchestrates_a_Primitive_Interferon-Like_Antiviral_Mechanism_in_Oyster_doc/14748771 |
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ftfrontimediafig:oai:figshare.com:article/14748771 2023-05-15T15:58:18+02:00 DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song 2021-06-08T13:35:31Z https://doi.org/10.3389/fimmu.2021.689783.s001 https://figshare.com/articles/dataset/DataSheet_1_The_cGAS_STING_TBK1_IRF_Regulatory_Axis_Orchestrates_a_Primitive_Interferon-Like_Antiviral_Mechanism_in_Oyster_doc/14748771 unknown doi:10.3389/fimmu.2021.689783.s001 https://figshare.com/articles/dataset/DataSheet_1_The_cGAS_STING_TBK1_IRF_Regulatory_Axis_Orchestrates_a_Primitive_Interferon-Like_Antiviral_Mechanism_in_Oyster_doc/14748771 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fimmu.2021.689783.s001 2021-06-09T22:57:49Z Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. Dataset Crassostrea gigas Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity |
description |
Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. |
format |
Dataset |
author |
Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song |
author_facet |
Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song |
author_sort |
Xue Qiao |
title |
DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc |
title_short |
DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc |
title_full |
DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc |
title_fullStr |
DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc |
title_full_unstemmed |
DataSheet_1_The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.doc |
title_sort |
datasheet_1_the cgas/sting–tbk1–irf regulatory axis orchestrates a primitive interferon-like antiviral mechanism in oyster.doc |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.689783.s001 https://figshare.com/articles/dataset/DataSheet_1_The_cGAS_STING_TBK1_IRF_Regulatory_Axis_Orchestrates_a_Primitive_Interferon-Like_Antiviral_Mechanism_in_Oyster_doc/14748771 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
doi:10.3389/fimmu.2021.689783.s001 https://figshare.com/articles/dataset/DataSheet_1_The_cGAS_STING_TBK1_IRF_Regulatory_Axis_Orchestrates_a_Primitive_Interferon-Like_Antiviral_Mechanism_in_Oyster_doc/14748771 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.689783.s001 |
_version_ |
1766394036011139072 |