Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP
The formation of hybrid sterility is an important stage of speciation. The voles of the genus Microtus, which is the most speciose genus of rodents, provide a good model for studying the cytological mechanisms of hybrid sterility. The voles of the “mystacinus” group of the subgenus Microtus (2n = 54...
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2021
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ftfrontimediafig:oai:figshare.com:article/14564085 2023-05-15T17:12:40+02:00 Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP Tatiana I. Bikchurina Fedor N. Golenishchev Elena A. Kizilova Ahmad Mahmoudi Pavel M. Borodin 2021-05-10T05:32:27Z https://doi.org/10.3389/fgene.2021.653837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Reproductive_Isolation_Between_Taxonomically_Controversial_Forms_of_the_Gray_Voles_Microtus_Rodentia_Arvicolinae_Cytological_Mechanisms_and_Taxonomical_Implications_ZIP/14564085 unknown doi:10.3389/fgene.2021.653837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Reproductive_Isolation_Between_Taxonomically_Controversial_Forms_of_the_Gray_Voles_Microtus_Rodentia_Arvicolinae_Cytological_Mechanisms_and_Taxonomical_Implications_ZIP/14564085 CC BY 4.0 CC-BY Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy voles meiotic abnormalities hybrid sterility reproductive isolation taxonomic status Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fgene.2021.653837.s001 2021-05-12T22:58:32Z The formation of hybrid sterility is an important stage of speciation. The voles of the genus Microtus, which is the most speciose genus of rodents, provide a good model for studying the cytological mechanisms of hybrid sterility. The voles of the “mystacinus” group of the subgenus Microtus (2n = 54) comprising several recently diverged forms with unclear taxonomic status are especially interesting. To resolve the taxonomic status of Microtus mystacinus and Microtus kermanensis, we crossed both with Microtus rossiaemeridionalis, and M. kermanensis alone with Microtus arvalis “obscurus” and M. transcaspicus and examined the reproductive performance of their F1 hybrids. All interspecies male hybrids were sterile. Female M. kermanensis × M. arvalis and M. kermanensis × M. transcaspicus hybrids were sterile as well. Therefore, M. mystacinus, M. kermanensis, and M. rossiaemeridionalis could be considered valid species. To gain an insight into the cytological mechanisms of male hybrid sterility, we carried out a histological analysis of spermatogenesis and a cytological analysis of chromosome synapsis, recombination, and epigenetic chromatin modifications in the germ cells of the hybrids using immunolocalization of key meiotic proteins. The hybrids showed wide variation in the onset of spermatogenesis arrest stage, from mature (although abnormal) spermatozoa to spermatogonia only. Chromosome asynapsis was apparently the main cause of meiotic arrest. The degree of asynapsis varied widely across cells, individuals, and the crosses—from partial asynapsis of several small bivalents to complete asynapsis of all chromosomes. The asynapsis was accompanied by a delayed repair of DNA double-strand breaks marked by RAD51 antibodies and silencing of unpaired chromatin marked by γH2A.X antibodies. Overall, the severity of disturbances in spermatogenesis in general and in chromosome synapsis in particular increased in the hybrids with an increase in the phylogenetic distance between their parental species. Dataset Microtus arvalis Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy voles meiotic abnormalities hybrid sterility reproductive isolation taxonomic status |
spellingShingle |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy voles meiotic abnormalities hybrid sterility reproductive isolation taxonomic status Tatiana I. Bikchurina Fedor N. Golenishchev Elena A. Kizilova Ahmad Mahmoudi Pavel M. Borodin Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP |
topic_facet |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy voles meiotic abnormalities hybrid sterility reproductive isolation taxonomic status |
description |
The formation of hybrid sterility is an important stage of speciation. The voles of the genus Microtus, which is the most speciose genus of rodents, provide a good model for studying the cytological mechanisms of hybrid sterility. The voles of the “mystacinus” group of the subgenus Microtus (2n = 54) comprising several recently diverged forms with unclear taxonomic status are especially interesting. To resolve the taxonomic status of Microtus mystacinus and Microtus kermanensis, we crossed both with Microtus rossiaemeridionalis, and M. kermanensis alone with Microtus arvalis “obscurus” and M. transcaspicus and examined the reproductive performance of their F1 hybrids. All interspecies male hybrids were sterile. Female M. kermanensis × M. arvalis and M. kermanensis × M. transcaspicus hybrids were sterile as well. Therefore, M. mystacinus, M. kermanensis, and M. rossiaemeridionalis could be considered valid species. To gain an insight into the cytological mechanisms of male hybrid sterility, we carried out a histological analysis of spermatogenesis and a cytological analysis of chromosome synapsis, recombination, and epigenetic chromatin modifications in the germ cells of the hybrids using immunolocalization of key meiotic proteins. The hybrids showed wide variation in the onset of spermatogenesis arrest stage, from mature (although abnormal) spermatozoa to spermatogonia only. Chromosome asynapsis was apparently the main cause of meiotic arrest. The degree of asynapsis varied widely across cells, individuals, and the crosses—from partial asynapsis of several small bivalents to complete asynapsis of all chromosomes. The asynapsis was accompanied by a delayed repair of DNA double-strand breaks marked by RAD51 antibodies and silencing of unpaired chromatin marked by γH2A.X antibodies. Overall, the severity of disturbances in spermatogenesis in general and in chromosome synapsis in particular increased in the hybrids with an increase in the phylogenetic distance between their parental species. |
format |
Dataset |
author |
Tatiana I. Bikchurina Fedor N. Golenishchev Elena A. Kizilova Ahmad Mahmoudi Pavel M. Borodin |
author_facet |
Tatiana I. Bikchurina Fedor N. Golenishchev Elena A. Kizilova Ahmad Mahmoudi Pavel M. Borodin |
author_sort |
Tatiana I. Bikchurina |
title |
Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP |
title_short |
Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP |
title_full |
Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP |
title_fullStr |
Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP |
title_full_unstemmed |
Data_Sheet_1_Reproductive Isolation Between Taxonomically Controversial Forms of the Gray Voles (Microtus, Rodentia; Arvicolinae): Cytological Mechanisms and Taxonomical Implications.ZIP |
title_sort |
data_sheet_1_reproductive isolation between taxonomically controversial forms of the gray voles (microtus, rodentia; arvicolinae): cytological mechanisms and taxonomical implications.zip |
publishDate |
2021 |
url |
https://doi.org/10.3389/fgene.2021.653837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Reproductive_Isolation_Between_Taxonomically_Controversial_Forms_of_the_Gray_Voles_Microtus_Rodentia_Arvicolinae_Cytological_Mechanisms_and_Taxonomical_Implications_ZIP/14564085 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_relation |
doi:10.3389/fgene.2021.653837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Reproductive_Isolation_Between_Taxonomically_Controversial_Forms_of_the_Gray_Voles_Microtus_Rodentia_Arvicolinae_Cytological_Mechanisms_and_Taxonomical_Implications_ZIP/14564085 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fgene.2021.653837.s001 |
_version_ |
1766069455357476864 |