Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx
Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill fun...
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ftfrontimediafig:oai:figshare.com:article/14533254 2023-05-15T15:30:56+02:00 Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx Alexander C. West Yasutaka Mizoro Shona H. Wood Louise M. Ince Marianne Iversen Even H. Jørgensen Torfinn Nome Simen Rød Sandve Samuel A. M. Martin Andrew S. I. Loudon David G. Hazlerigg 2021-05-04T04:48:54Z https://doi.org/10.3389/fimmu.2021.669889.s002 https://figshare.com/articles/dataset/Table_1_Immunologic_Profiling_of_the_Atlantic_Salmon_Gill_by_Single_Nuclei_Transcriptomics_xlsx/14533254 unknown doi:10.3389/fimmu.2021.669889.s002 https://figshare.com/articles/dataset/Table_1_Immunologic_Profiling_of_the_Atlantic_Salmon_Gill_by_Single_Nuclei_Transcriptomics_xlsx/14533254 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon (Salmo salar) smoltification photoperiod immune cells gill single nuclei RNA sequencing Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fimmu.2021.669889.s002 2021-05-05T22:57:53Z Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill function to cope with the profound osmotic and immunological challenges of seawater (SW) migration. While prior work has highlighted the role of specialized “mitochondrion-rich” cells (MRCs) and accessory cells (ACs) in delivering this phenotype, recent RNA profiling experiments suggest that remodeling is far more extensive than previously appreciated. Here, we use single-nuclei RNAseq to characterize the extent of cytological changes in the gill of Atlantic salmon during smoltification and SW transfer. We identify 20 distinct cell clusters, including known, but also novel gill cell types. These data allow us to isolate cluster-specific, smoltification-associated changes in gene expression and to describe how the cellular make-up of the gill changes through smoltification. As expected, we noted an increase in the proportion of seawater mitochondrion-rich cells, however, we also identify previously unknown reduction of several immune-related cell types. Overall, our results provide fresh detail of the cellular complexity in the gill and suggest that smoltification triggers unexpected immune reprogramming. Dataset Atlantic salmon Salmo salar Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon (Salmo salar) smoltification photoperiod immune cells gill single nuclei RNA sequencing |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon (Salmo salar) smoltification photoperiod immune cells gill single nuclei RNA sequencing Alexander C. West Yasutaka Mizoro Shona H. Wood Louise M. Ince Marianne Iversen Even H. Jørgensen Torfinn Nome Simen Rød Sandve Samuel A. M. Martin Andrew S. I. Loudon David G. Hazlerigg Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon (Salmo salar) smoltification photoperiod immune cells gill single nuclei RNA sequencing |
description |
Anadromous salmonids begin life adapted to the freshwater environments of their natal streams before a developmental transition, known as smoltification, transforms them into marine-adapted fish. In the wild, smoltification is a photoperiod-regulated process, involving radical remodeling of gill function to cope with the profound osmotic and immunological challenges of seawater (SW) migration. While prior work has highlighted the role of specialized “mitochondrion-rich” cells (MRCs) and accessory cells (ACs) in delivering this phenotype, recent RNA profiling experiments suggest that remodeling is far more extensive than previously appreciated. Here, we use single-nuclei RNAseq to characterize the extent of cytological changes in the gill of Atlantic salmon during smoltification and SW transfer. We identify 20 distinct cell clusters, including known, but also novel gill cell types. These data allow us to isolate cluster-specific, smoltification-associated changes in gene expression and to describe how the cellular make-up of the gill changes through smoltification. As expected, we noted an increase in the proportion of seawater mitochondrion-rich cells, however, we also identify previously unknown reduction of several immune-related cell types. Overall, our results provide fresh detail of the cellular complexity in the gill and suggest that smoltification triggers unexpected immune reprogramming. |
format |
Dataset |
author |
Alexander C. West Yasutaka Mizoro Shona H. Wood Louise M. Ince Marianne Iversen Even H. Jørgensen Torfinn Nome Simen Rød Sandve Samuel A. M. Martin Andrew S. I. Loudon David G. Hazlerigg |
author_facet |
Alexander C. West Yasutaka Mizoro Shona H. Wood Louise M. Ince Marianne Iversen Even H. Jørgensen Torfinn Nome Simen Rød Sandve Samuel A. M. Martin Andrew S. I. Loudon David G. Hazlerigg |
author_sort |
Alexander C. West |
title |
Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx |
title_short |
Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx |
title_full |
Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx |
title_fullStr |
Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx |
title_full_unstemmed |
Table_1_Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.xlsx |
title_sort |
table_1_immunologic profiling of the atlantic salmon gill by single nuclei transcriptomics.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.669889.s002 https://figshare.com/articles/dataset/Table_1_Immunologic_Profiling_of_the_Atlantic_Salmon_Gill_by_Single_Nuclei_Transcriptomics_xlsx/14533254 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fimmu.2021.669889.s002 https://figshare.com/articles/dataset/Table_1_Immunologic_Profiling_of_the_Atlantic_Salmon_Gill_by_Single_Nuclei_Transcriptomics_xlsx/14533254 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.669889.s002 |
_version_ |
1766361419388813312 |