Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX
Entering meiosis strictly depends on stimulated by retinoic acid 8 (Stra8) gene function in mammals. This gene is missing in a number of fish species, including medaka and zebrafish, but is present in the majority of fishes, including Atlantic salmon. Here, we have examined the effects of removing s...
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ftfrontimediafig:oai:figshare.com:article/14439020 2023-05-15T15:31:28+02:00 Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX Kai O. Skaftnesmo Diego Crespo Lene Kleppe Eva Andersson Rolf B. Edvardsen Birgitta Norberg Per Gunnar Fjelldal Tom J. Hansen Rüdiger W. Schulz Anna Wargelius 2021-04-16T14:01:50Z https://doi.org/10.3389/fcell.2021.657192.s002 https://figshare.com/articles/dataset/Table_2_Loss_of_stra8_Increases_Germ_Cell_Apoptosis_but_Is_Still_Compatible_With_Sperm_Production_in_Atlantic_Salmon_Salmo_salar_DOCX/14439020 unknown doi:10.3389/fcell.2021.657192.s002 https://figshare.com/articles/dataset/Table_2_Loss_of_stra8_Increases_Germ_Cell_Apoptosis_but_Is_Still_Compatible_With_Sperm_Production_in_Atlantic_Salmon_Salmo_salar_DOCX/14439020 CC BY 4.0 CC-BY Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) stimulated by retinoic acid 8 gene editing spermatogenesis apoptosis single cell proliferation Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fcell.2021.657192.s002 2021-04-21T22:59:19Z Entering meiosis strictly depends on stimulated by retinoic acid 8 (Stra8) gene function in mammals. This gene is missing in a number of fish species, including medaka and zebrafish, but is present in the majority of fishes, including Atlantic salmon. Here, we have examined the effects of removing stra8 on male fertility in Atlantic salmon. As in mammals, stra8 expression was restricted to germ cells in the testis, transcript levels increased during the start of puberty, and decreased when blocking the production of retinoic acid. We targeted the salmon stra8 gene with two gRNAs one of these were highly effective and produced numerous mutations in stra8, which led to a loss of wild-type (WT) stra8 expression in F0 salmon testis. In maturing stra8 crispants, the spermatogenetic tubuli were partially disorganized and displayed a sevenfold increase in germ cell apoptosis, in particular among type B spermatogonia and spermatocytes. The production of spermatogenic cysts, on the other hand, increased in maturing stra8 crispants. Gene expression analysis revealed unchanged (lin28a, ret) or reduced levels (egr1, dusp4) of transcripts associated with undifferentiated spermatogonia. Decreased expression was recorded for some genes expressed in differentiating spermatogonia including dmrt1 and ccnd2 or in spermatocytes, such as ccna1. Different from Stra8-deficient mammals, a large number of germ cells completed spermatogenesis, sperm was produced and fertilization rates were similar in WT and crispant males. While loss of stra8 increased germ cell apoptosis during salmon spermatogenesis, crispants compensated this cell loss by an elevated production of spermatogenic cysts, and were able to produce functional sperm. It appears that also in a fish species with a stra8 gene in the genome, the critical relevance this gene has attained for mammalian spermatogenesis is not yet given, although detrimental effects of the loss of stra8 were clearly visible during maturation. Dataset Atlantic salmon Salmo salar Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) stimulated by retinoic acid 8 gene editing spermatogenesis apoptosis single cell proliferation |
spellingShingle |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) stimulated by retinoic acid 8 gene editing spermatogenesis apoptosis single cell proliferation Kai O. Skaftnesmo Diego Crespo Lene Kleppe Eva Andersson Rolf B. Edvardsen Birgitta Norberg Per Gunnar Fjelldal Tom J. Hansen Rüdiger W. Schulz Anna Wargelius Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX |
topic_facet |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) stimulated by retinoic acid 8 gene editing spermatogenesis apoptosis single cell proliferation |
description |
Entering meiosis strictly depends on stimulated by retinoic acid 8 (Stra8) gene function in mammals. This gene is missing in a number of fish species, including medaka and zebrafish, but is present in the majority of fishes, including Atlantic salmon. Here, we have examined the effects of removing stra8 on male fertility in Atlantic salmon. As in mammals, stra8 expression was restricted to germ cells in the testis, transcript levels increased during the start of puberty, and decreased when blocking the production of retinoic acid. We targeted the salmon stra8 gene with two gRNAs one of these were highly effective and produced numerous mutations in stra8, which led to a loss of wild-type (WT) stra8 expression in F0 salmon testis. In maturing stra8 crispants, the spermatogenetic tubuli were partially disorganized and displayed a sevenfold increase in germ cell apoptosis, in particular among type B spermatogonia and spermatocytes. The production of spermatogenic cysts, on the other hand, increased in maturing stra8 crispants. Gene expression analysis revealed unchanged (lin28a, ret) or reduced levels (egr1, dusp4) of transcripts associated with undifferentiated spermatogonia. Decreased expression was recorded for some genes expressed in differentiating spermatogonia including dmrt1 and ccnd2 or in spermatocytes, such as ccna1. Different from Stra8-deficient mammals, a large number of germ cells completed spermatogenesis, sperm was produced and fertilization rates were similar in WT and crispant males. While loss of stra8 increased germ cell apoptosis during salmon spermatogenesis, crispants compensated this cell loss by an elevated production of spermatogenic cysts, and were able to produce functional sperm. It appears that also in a fish species with a stra8 gene in the genome, the critical relevance this gene has attained for mammalian spermatogenesis is not yet given, although detrimental effects of the loss of stra8 were clearly visible during maturation. |
format |
Dataset |
author |
Kai O. Skaftnesmo Diego Crespo Lene Kleppe Eva Andersson Rolf B. Edvardsen Birgitta Norberg Per Gunnar Fjelldal Tom J. Hansen Rüdiger W. Schulz Anna Wargelius |
author_facet |
Kai O. Skaftnesmo Diego Crespo Lene Kleppe Eva Andersson Rolf B. Edvardsen Birgitta Norberg Per Gunnar Fjelldal Tom J. Hansen Rüdiger W. Schulz Anna Wargelius |
author_sort |
Kai O. Skaftnesmo |
title |
Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX |
title_short |
Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX |
title_full |
Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX |
title_fullStr |
Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX |
title_full_unstemmed |
Table_2_Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar).DOCX |
title_sort |
table_2_loss of stra8 increases germ cell apoptosis but is still compatible with sperm production in atlantic salmon (salmo salar).docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fcell.2021.657192.s002 https://figshare.com/articles/dataset/Table_2_Loss_of_stra8_Increases_Germ_Cell_Apoptosis_but_Is_Still_Compatible_With_Sperm_Production_in_Atlantic_Salmon_Salmo_salar_DOCX/14439020 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fcell.2021.657192.s002 https://figshare.com/articles/dataset/Table_2_Loss_of_stra8_Increases_Germ_Cell_Apoptosis_but_Is_Still_Compatible_With_Sperm_Production_in_Atlantic_Salmon_Salmo_salar_DOCX/14439020 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fcell.2021.657192.s002 |
_version_ |
1766361979654504448 |