Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX
Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster Crassostrea gigas, ha...
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ftfrontimediafig:oai:figshare.com:article/14386379 2023-05-15T15:57:42+02:00 Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX Kaidi Jin Qianqian Jin Zhongqiang Cai Baoyu Huang Lei Wei Meiwei Zhang Wen Guo Yaqiong Liu Xiaotong Wang 2021-04-08T05:06:47Z https://doi.org/10.3389/fphys.2021.666842.s001 https://figshare.com/articles/dataset/Table_1_Molecular_Characterization_of_Retinoic_Acid_Receptor_CgRAR_in_Pacific_Oyster_Crassostrea_gigas_DOCX/14386379 unknown doi:10.3389/fphys.2021.666842.s001 https://figshare.com/articles/dataset/Table_1_Molecular_Characterization_of_Retinoic_Acid_Receptor_CgRAR_in_Pacific_Oyster_Crassostrea_gigas_DOCX/14386379 CC BY 4.0 CC-BY Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Crassostrea gigas retinoic acid receptor retinoid X receptor molecular characterization retinoic acid Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fphys.2021.666842.s001 2021-04-14T23:01:01Z Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster Crassostrea gigas, have a different function mechanism compared with that in chordates. In this report, we identified the molecular characteristics of CgRAR to further explore the mechanism of RAR in mollusks. RT-qPCR analysis shows that CgRAR has a higher expression level in the hemocytes and gonads, indicating that CgRAR may play roles in the processes of development and metabolism. The mRNA expression level of both CgRAR and CgRXR was analyzed by RT-qPCR after injection with RA. The elevated expression of CgRAR and CgRXR was detected upon all-trans-RA (ATRA) exposure. Finally, according to the results of Yeast Two-Hybrid assay and co-immunoprecipitation analysis, CgRAR and CgRXR can interact with each other through the C-terminal region. Taken together, our results suggest that CgRAR shows a higher expression level in gonads and hemocytes. ATRA exposure up-regulates the expression of CgRAR and CgRXR. Besides, CgRAR can interact with CgRXR to form a heterodimer complex. Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Crassostrea gigas retinoic acid receptor retinoid X receptor molecular characterization retinoic acid |
spellingShingle |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Crassostrea gigas retinoic acid receptor retinoid X receptor molecular characterization retinoic acid Kaidi Jin Qianqian Jin Zhongqiang Cai Baoyu Huang Lei Wei Meiwei Zhang Wen Guo Yaqiong Liu Xiaotong Wang Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX |
topic_facet |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Crassostrea gigas retinoic acid receptor retinoid X receptor molecular characterization retinoic acid |
description |
Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster Crassostrea gigas, have a different function mechanism compared with that in chordates. In this report, we identified the molecular characteristics of CgRAR to further explore the mechanism of RAR in mollusks. RT-qPCR analysis shows that CgRAR has a higher expression level in the hemocytes and gonads, indicating that CgRAR may play roles in the processes of development and metabolism. The mRNA expression level of both CgRAR and CgRXR was analyzed by RT-qPCR after injection with RA. The elevated expression of CgRAR and CgRXR was detected upon all-trans-RA (ATRA) exposure. Finally, according to the results of Yeast Two-Hybrid assay and co-immunoprecipitation analysis, CgRAR and CgRXR can interact with each other through the C-terminal region. Taken together, our results suggest that CgRAR shows a higher expression level in gonads and hemocytes. ATRA exposure up-regulates the expression of CgRAR and CgRXR. Besides, CgRAR can interact with CgRXR to form a heterodimer complex. |
format |
Dataset |
author |
Kaidi Jin Qianqian Jin Zhongqiang Cai Baoyu Huang Lei Wei Meiwei Zhang Wen Guo Yaqiong Liu Xiaotong Wang |
author_facet |
Kaidi Jin Qianqian Jin Zhongqiang Cai Baoyu Huang Lei Wei Meiwei Zhang Wen Guo Yaqiong Liu Xiaotong Wang |
author_sort |
Kaidi Jin |
title |
Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX |
title_short |
Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX |
title_full |
Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX |
title_fullStr |
Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX |
title_full_unstemmed |
Table_1_Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas).DOCX |
title_sort |
table_1_molecular characterization of retinoic acid receptor cgrar in pacific oyster (crassostrea gigas).docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fphys.2021.666842.s001 https://figshare.com/articles/dataset/Table_1_Molecular_Characterization_of_Retinoic_Acid_Receptor_CgRAR_in_Pacific_Oyster_Crassostrea_gigas_DOCX/14386379 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fphys.2021.666842.s001 https://figshare.com/articles/dataset/Table_1_Molecular_Characterization_of_Retinoic_Acid_Receptor_CgRAR_in_Pacific_Oyster_Crassostrea_gigas_DOCX/14386379 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2021.666842.s001 |
_version_ |
1766393381392482304 |