Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx
The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain b...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2021.631174.s005 https://figshare.com/articles/dataset/Data_Sheet_5_Passive_Samplers_a_Powerful_Tool_to_Detect_Viruses_and_Bacteria_in_Marine_Coastal_Areas_docx/14088200 |
id |
ftfrontimediafig:oai:figshare.com:article/14088200 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/14088200 2023-05-15T15:58:56+02:00 Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx Françoise Vincent-Hubert Candice Wacrenier Benjamin Morga Solen Lozach Emmanuelle Quenot Mickaël Mège Cyrielle Lecadet Michèle Gourmelon Dominique Hervio-Heath Françoise S. Le Guyader 2021-02-23T04:19:43Z https://doi.org/10.3389/fmicb.2021.631174.s005 https://figshare.com/articles/dataset/Data_Sheet_5_Passive_Samplers_a_Powerful_Tool_to_Detect_Viruses_and_Bacteria_in_Marine_Coastal_Areas_docx/14088200 unknown doi:10.3389/fmicb.2021.631174.s005 https://figshare.com/articles/dataset/Data_Sheet_5_Passive_Samplers_a_Powerful_Tool_to_Detect_Viruses_and_Bacteria_in_Marine_Coastal_Areas_docx/14088200 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology norovirus Ostreid herpes virus 1 μvar Vibrio spp microbial source tracking sea passive sampler oyster (Crassostrea gigas) Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fmicb.2021.631174.s005 2021-02-24T23:59:12Z The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species Vibrio alginolyticus, V. cholerae, V. vulnificus, and V. parahaemolyticus, OsHV-1 virus, and bacterial markers of fecal contamination. NoV GII, Vibrio spp., and the AllBac general Bacteroidales marker were quantified on the three types of membrane. NoV GII and OsHV-1 viruses followed a seasonal distribution. All membranes were favorable for NoV GII detection, while Zetapor was more adapted for OsHV-1 detection. Nylon was more adapted for detection of Vibrio spp. and the AllBac marker. The quantities of NoV GII, AllBac, and Vibrio spp. recovered on membranes increased with the duration of exposure. This first application of passive sampling in seawater is particularly promising in terms of an early warning system for the prevention of contamination in oyster farming areas and to improve our knowledge on the timing and frequency of disease occurence. Dataset Crassostrea gigas Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology norovirus Ostreid herpes virus 1 μvar Vibrio spp microbial source tracking sea passive sampler oyster (Crassostrea gigas) |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology norovirus Ostreid herpes virus 1 μvar Vibrio spp microbial source tracking sea passive sampler oyster (Crassostrea gigas) Françoise Vincent-Hubert Candice Wacrenier Benjamin Morga Solen Lozach Emmanuelle Quenot Mickaël Mège Cyrielle Lecadet Michèle Gourmelon Dominique Hervio-Heath Françoise S. Le Guyader Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology norovirus Ostreid herpes virus 1 μvar Vibrio spp microbial source tracking sea passive sampler oyster (Crassostrea gigas) |
description |
The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species Vibrio alginolyticus, V. cholerae, V. vulnificus, and V. parahaemolyticus, OsHV-1 virus, and bacterial markers of fecal contamination. NoV GII, Vibrio spp., and the AllBac general Bacteroidales marker were quantified on the three types of membrane. NoV GII and OsHV-1 viruses followed a seasonal distribution. All membranes were favorable for NoV GII detection, while Zetapor was more adapted for OsHV-1 detection. Nylon was more adapted for detection of Vibrio spp. and the AllBac marker. The quantities of NoV GII, AllBac, and Vibrio spp. recovered on membranes increased with the duration of exposure. This first application of passive sampling in seawater is particularly promising in terms of an early warning system for the prevention of contamination in oyster farming areas and to improve our knowledge on the timing and frequency of disease occurence. |
format |
Dataset |
author |
Françoise Vincent-Hubert Candice Wacrenier Benjamin Morga Solen Lozach Emmanuelle Quenot Mickaël Mège Cyrielle Lecadet Michèle Gourmelon Dominique Hervio-Heath Françoise S. Le Guyader |
author_facet |
Françoise Vincent-Hubert Candice Wacrenier Benjamin Morga Solen Lozach Emmanuelle Quenot Mickaël Mège Cyrielle Lecadet Michèle Gourmelon Dominique Hervio-Heath Françoise S. Le Guyader |
author_sort |
Françoise Vincent-Hubert |
title |
Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx |
title_short |
Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx |
title_full |
Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx |
title_fullStr |
Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx |
title_full_unstemmed |
Data_Sheet_5_Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas.docx |
title_sort |
data_sheet_5_passive samplers, a powerful tool to detect viruses and bacteria in marine coastal areas.docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.631174.s005 https://figshare.com/articles/dataset/Data_Sheet_5_Passive_Samplers_a_Powerful_Tool_to_Detect_Viruses_and_Bacteria_in_Marine_Coastal_Areas_docx/14088200 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
doi:10.3389/fmicb.2021.631174.s005 https://figshare.com/articles/dataset/Data_Sheet_5_Passive_Samplers_a_Powerful_Tool_to_Detect_Viruses_and_Bacteria_in_Marine_Coastal_Areas_docx/14088200 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.631174.s005 |
_version_ |
1766394707241336832 |