Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx
Background Invasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging t...
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ftfrontimediafig:oai:figshare.com:article/14058692 2023-05-15T16:51:07+02:00 Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo 2021-02-19T04:22:50Z https://doi.org/10.3389/fmicb.2021.637813.s002 https://figshare.com/articles/dataset/Table_1_The_Emergence_of_Invasive_Streptococcus_pneumoniae_Serotype_24F_in_Lebanon_Complete_Genome_Sequencing_Reveals_High_Virulence_and_Antimicrobial_Resistance_Characteristics_xlsx/14058692 unknown doi:10.3389/fmicb.2021.637813.s002 https://figshare.com/articles/dataset/Table_1_The_Emergence_of_Invasive_Streptococcus_pneumoniae_Serotype_24F_in_Lebanon_Complete_Genome_Sequencing_Reveals_High_Virulence_and_Antimicrobial_Resistance_Characteristics_xlsx/14058692 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fmicb.2021.637813.s002 2021-02-25T00:01:53Z Background Invasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging to a non-PCV serotype, were detected through the Lebanese Inter-Hospital Pneumococcal Surveillance Program. The aim of the study is to characterize phenotypic and genomic features of the 24F isolates in Lebanon. Methods WGS using long reads sequencing (PacBio) was performed to produce complete circular genomes and to determine clonality, antimicrobial resistance and virulence determinants. Results The sequencing results yielded eight closed circular genomes. Three multilocus sequence typing (MLST) types were identified (ST11618, ST14184, ST15253). Both MLST and WGS analyses revealed that these isolates from Lebanon were genetically homogenous belonging to clonal complex CC230 and clustered closely with isolates originating from Canada, United States of America, United Kingdom and Iceland. Their penicillin binding protein profiles correlated with both β-lactam susceptibility patterns and MLST types. Moreover, the isolates harbored the macrolide and tetracycline resistance genes and showed a similar virulence gene profile. To our knowledge, this study represents the first report of complete phenotypic and genomic characterization of the emerging Streptococcus pneumoniae, serotype 24F, in the Middle East and North Africa region. Dataset Iceland Frontiers: Figshare Canada |
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Frontiers: Figshare |
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Microbiology Microbial Genetics Microbial Ecology Mycology Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance |
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Microbiology Microbial Genetics Microbial Ecology Mycology Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx |
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Microbiology Microbial Genetics Microbial Ecology Mycology Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance |
description |
Background Invasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging to a non-PCV serotype, were detected through the Lebanese Inter-Hospital Pneumococcal Surveillance Program. The aim of the study is to characterize phenotypic and genomic features of the 24F isolates in Lebanon. Methods WGS using long reads sequencing (PacBio) was performed to produce complete circular genomes and to determine clonality, antimicrobial resistance and virulence determinants. Results The sequencing results yielded eight closed circular genomes. Three multilocus sequence typing (MLST) types were identified (ST11618, ST14184, ST15253). Both MLST and WGS analyses revealed that these isolates from Lebanon were genetically homogenous belonging to clonal complex CC230 and clustered closely with isolates originating from Canada, United States of America, United Kingdom and Iceland. Their penicillin binding protein profiles correlated with both β-lactam susceptibility patterns and MLST types. Moreover, the isolates harbored the macrolide and tetracycline resistance genes and showed a similar virulence gene profile. To our knowledge, this study represents the first report of complete phenotypic and genomic characterization of the emerging Streptococcus pneumoniae, serotype 24F, in the Middle East and North Africa region. |
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author |
Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo |
author_facet |
Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo |
author_sort |
Lina Reslan |
title |
Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx |
title_short |
Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx |
title_full |
Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx |
title_fullStr |
Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx |
title_full_unstemmed |
Table_1_The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics.xlsx |
title_sort |
table_1_the emergence of invasive streptococcus pneumoniae serotype 24f in lebanon: complete genome sequencing reveals high virulence and antimicrobial resistance characteristics.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.637813.s002 https://figshare.com/articles/dataset/Table_1_The_Emergence_of_Invasive_Streptococcus_pneumoniae_Serotype_24F_in_Lebanon_Complete_Genome_Sequencing_Reveals_High_Virulence_and_Antimicrobial_Resistance_Characteristics_xlsx/14058692 |
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Canada |
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Iceland |
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Iceland |
op_relation |
doi:10.3389/fmicb.2021.637813.s002 https://figshare.com/articles/dataset/Table_1_The_Emergence_of_Invasive_Streptococcus_pneumoniae_Serotype_24F_in_Lebanon_Complete_Genome_Sequencing_Reveals_High_Virulence_and_Antimicrobial_Resistance_Characteristics_xlsx/14058692 |
op_rights |
CC BY 4.0 |
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CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2021.637813.s002 |
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1766041237979136000 |