DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx
DM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fimmu.2020.603270.s001 https://figshare.com/articles/dataset/DataSheet_1_Identification_of_a_Novel_Pattern_Recognition_Receptor_DM9_Domain_Containing_Protein_4_as_a_Marker_for_Pro-Hemocyte_of_Pacific_Oyster_Crassostrea_gigas_docx/13941653 |
id |
ftfrontimediafig:oai:figshare.com:article/13941653 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/13941653 2023-05-15T15:58:31+02:00 DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx Zhihao Jia Shuai Jiang Mengqiang Wang Xiudan Wang Yu Liu Zhao Lv Xiaorui Song Yiqun Li Lingling Wang Linsheng Song 2021-02-12T05:28:04Z https://doi.org/10.3389/fimmu.2020.603270.s001 https://figshare.com/articles/dataset/DataSheet_1_Identification_of_a_Novel_Pattern_Recognition_Receptor_DM9_Domain_Containing_Protein_4_as_a_Marker_for_Pro-Hemocyte_of_Pacific_Oyster_Crassostrea_gigas_docx/13941653 unknown doi:10.3389/fimmu.2020.603270.s001 https://figshare.com/articles/dataset/DataSheet_1_Identification_of_a_Novel_Pattern_Recognition_Receptor_DM9_Domain_Containing_Protein_4_as_a_Marker_for_Pro-Hemocyte_of_Pacific_Oyster_Crassostrea_gigas_docx/13941653 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.603270.s001 2021-02-17T23:58:54Z DM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel member of DM9 domain containing protein (designated as CgDM9CP-4) was identified from C. gigas. CgDM9CP-4, about 16 kDa with only two tandem DM9 domains, was highly enriched in hemocytes and gill. The transcripts level of CgDM9CP-4 in circulating hemocytes were decreased after LPS, PGN and Vibrio splendidus stimulations. The recombinant protein of CgDM9CP-4 (rCgDM9CP-4) displayed a broad binding spectrum towards various pathogen-associated molecular patterns (PAMPs) (LPS, PGN, β-glucan and Mannose) and microorganisms (Staphylococcus aureus, Micrococcus luteus, V. splendidus, V. anguillarum, Escherichia coli, Pichia pastoris and Yarrowia lipolytica). CgDM9CP-4 was mostly expressed in gill and some of the hemocytes. Flow cytometry analysis demonstrated that the CgDM9CP-4-positive hemocytes accounted for 7.3% of the total hemocytes, and they were small in size and less in granularity. CgDM9CP-4 was highly expressed in non-phagocytes (~82% of total hemocytes). The reactive oxygen species (ROS) and the expression levels of cytokines in CgDM9CP-4-positive hemocytes were much lower than that in CgDM9CP-4-negative hemocytes. The mRNA expression level of CgDM9CP-4 in hemocytes was decreased after RNAi of hematopoietic-related factors (CgGATA, CgRunt, CgSCL, and CgNotch). In addition, CgDM9CP-4-positive cells were found to be much more abundant in hemocytes from gill than that from hemolymph, with most of them located in the gill filament. All these results suggested that CgDM9CP-4 was a novel member of PRR that expressed in undifferentiated pro-hemocytes to mediate immune recognition of pathogens. Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes Zhihao Jia Shuai Jiang Mengqiang Wang Xiudan Wang Yu Liu Zhao Lv Xiaorui Song Yiqun Li Lingling Wang Linsheng Song DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes |
description |
DM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel member of DM9 domain containing protein (designated as CgDM9CP-4) was identified from C. gigas. CgDM9CP-4, about 16 kDa with only two tandem DM9 domains, was highly enriched in hemocytes and gill. The transcripts level of CgDM9CP-4 in circulating hemocytes were decreased after LPS, PGN and Vibrio splendidus stimulations. The recombinant protein of CgDM9CP-4 (rCgDM9CP-4) displayed a broad binding spectrum towards various pathogen-associated molecular patterns (PAMPs) (LPS, PGN, β-glucan and Mannose) and microorganisms (Staphylococcus aureus, Micrococcus luteus, V. splendidus, V. anguillarum, Escherichia coli, Pichia pastoris and Yarrowia lipolytica). CgDM9CP-4 was mostly expressed in gill and some of the hemocytes. Flow cytometry analysis demonstrated that the CgDM9CP-4-positive hemocytes accounted for 7.3% of the total hemocytes, and they were small in size and less in granularity. CgDM9CP-4 was highly expressed in non-phagocytes (~82% of total hemocytes). The reactive oxygen species (ROS) and the expression levels of cytokines in CgDM9CP-4-positive hemocytes were much lower than that in CgDM9CP-4-negative hemocytes. The mRNA expression level of CgDM9CP-4 in hemocytes was decreased after RNAi of hematopoietic-related factors (CgGATA, CgRunt, CgSCL, and CgNotch). In addition, CgDM9CP-4-positive cells were found to be much more abundant in hemocytes from gill than that from hemolymph, with most of them located in the gill filament. All these results suggested that CgDM9CP-4 was a novel member of PRR that expressed in undifferentiated pro-hemocytes to mediate immune recognition of pathogens. |
format |
Dataset |
author |
Zhihao Jia Shuai Jiang Mengqiang Wang Xiudan Wang Yu Liu Zhao Lv Xiaorui Song Yiqun Li Lingling Wang Linsheng Song |
author_facet |
Zhihao Jia Shuai Jiang Mengqiang Wang Xiudan Wang Yu Liu Zhao Lv Xiaorui Song Yiqun Li Lingling Wang Linsheng Song |
author_sort |
Zhihao Jia |
title |
DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx |
title_short |
DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx |
title_full |
DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx |
title_fullStr |
DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx |
title_full_unstemmed |
DataSheet_1_Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas.docx |
title_sort |
datasheet_1_identification of a novel pattern recognition receptor dm9 domain containing protein 4 as a marker for pro-hemocyte of pacific oyster crassostrea gigas.docx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2020.603270.s001 https://figshare.com/articles/dataset/DataSheet_1_Identification_of_a_Novel_Pattern_Recognition_Receptor_DM9_Domain_Containing_Protein_4_as_a_Marker_for_Pro-Hemocyte_of_Pacific_Oyster_Crassostrea_gigas_docx/13941653 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2020.603270.s001 https://figshare.com/articles/dataset/DataSheet_1_Identification_of_a_Novel_Pattern_Recognition_Receptor_DM9_Domain_Containing_Protein_4_as_a_Marker_for_Pro-Hemocyte_of_Pacific_Oyster_Crassostrea_gigas_docx/13941653 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.603270.s001 |
_version_ |
1766394278838272000 |