Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx
Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted P...
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Online Access: | https://doi.org/10.3389/fimmu.2020.621994.s001 https://figshare.com/articles/dataset/Table_1_Comparative_Proteomics_of_Ostreid_Herpesvirus_1_and_Pacific_Oyster_Interactions_With_Two_Families_Exhibiting_Contrasted_Susceptibility_to_Viral_Infection_xlsx/13602059 |
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ftfrontimediafig:oai:figshare.com:article/13602059 2023-05-15T15:59:07+02:00 Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga 2021-01-18T04:49:50Z https://doi.org/10.3389/fimmu.2020.621994.s001 https://figshare.com/articles/dataset/Table_1_Comparative_Proteomics_of_Ostreid_Herpesvirus_1_and_Pacific_Oyster_Interactions_With_Two_Families_Exhibiting_Contrasted_Susceptibility_to_Viral_Infection_xlsx/13602059 unknown doi:10.3389/fimmu.2020.621994.s001 https://figshare.com/articles/dataset/Table_1_Comparative_Proteomics_of_Ostreid_Herpesvirus_1_and_Pacific_Oyster_Interactions_With_Two_Families_Exhibiting_Contrasted_Susceptibility_to_Viral_Infection_xlsx/13602059 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology proteomics interactions antiviral response OsHV-1 Pacific oysters Dataset 2021 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.621994.s001 2021-01-20T23:58:41Z Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and viral load observed in this oyster family. Concerning the Pacific oyster proteome, more than 3,000 proteins were identified and contrasted proteomic responses were observed between infected A- and P-oysters, sampled at different post-injection times. Gene ontology (GO) and KEGG pathway enrichment analysis performed on significantly modulated proteins uncover the main immune processes (such as RNA interference, interferon-like pathway, antioxidant defense) which contribute to the defense and resistance of Pacific oysters to viral infection. In the more susceptible Pacific oysters, results suggest that OsHV-1 manipulate the molecular machinery of host immune response, in particular the autophagy system. This immunomodulation may lead to weakening and consecutively triggering death of Pacific oysters. The identification of several highly modulated and defense-related Pacific oyster proteins from the most resistant oysters supports the crucial role played by the innate immune system against OsHV-1 and the viral infection. Our results confirm the implication of proteins involved in an interferon-like pathway for efficient antiviral defenses and suggest that proteins involved in RNA interference process prevent viral replication in C. gigas. Overall, this study shows ... Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology proteomics interactions antiviral response OsHV-1 Pacific oysters |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology proteomics interactions antiviral response OsHV-1 Pacific oysters Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology proteomics interactions antiviral response OsHV-1 Pacific oysters |
description |
Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and viral load observed in this oyster family. Concerning the Pacific oyster proteome, more than 3,000 proteins were identified and contrasted proteomic responses were observed between infected A- and P-oysters, sampled at different post-injection times. Gene ontology (GO) and KEGG pathway enrichment analysis performed on significantly modulated proteins uncover the main immune processes (such as RNA interference, interferon-like pathway, antioxidant defense) which contribute to the defense and resistance of Pacific oysters to viral infection. In the more susceptible Pacific oysters, results suggest that OsHV-1 manipulate the molecular machinery of host immune response, in particular the autophagy system. This immunomodulation may lead to weakening and consecutively triggering death of Pacific oysters. The identification of several highly modulated and defense-related Pacific oyster proteins from the most resistant oysters supports the crucial role played by the innate immune system against OsHV-1 and the viral infection. Our results confirm the implication of proteins involved in an interferon-like pathway for efficient antiviral defenses and suggest that proteins involved in RNA interference process prevent viral replication in C. gigas. Overall, this study shows ... |
format |
Dataset |
author |
Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga |
author_facet |
Maxime Leprêtre Nicole Faury Amélie Segarra Stéphane Claverol Lionel Degremont Mélissa Palos-Ladeiro Jean Armengaud Tristan Renault Benjamin Morga |
author_sort |
Maxime Leprêtre |
title |
Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx |
title_short |
Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx |
title_full |
Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx |
title_fullStr |
Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx |
title_full_unstemmed |
Table_1_Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection.xlsx |
title_sort |
table_1_comparative proteomics of ostreid herpesvirus 1 and pacific oyster interactions with two families exhibiting contrasted susceptibility to viral infection.xlsx |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2020.621994.s001 https://figshare.com/articles/dataset/Table_1_Comparative_Proteomics_of_Ostreid_Herpesvirus_1_and_Pacific_Oyster_Interactions_With_Two_Families_Exhibiting_Contrasted_Susceptibility_to_Viral_Infection_xlsx/13602059 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2020.621994.s001 https://figshare.com/articles/dataset/Table_1_Comparative_Proteomics_of_Ostreid_Herpesvirus_1_and_Pacific_Oyster_Interactions_With_Two_Families_Exhibiting_Contrasted_Susceptibility_to_Viral_Infection_xlsx/13602059 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.621994.s001 |
_version_ |
1766394906233798656 |