Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf
Turbot (Scophthalmus maximus) is an important marine fish both in Europe and North China. Although there are plenty of studies on the reproduction of turbot, the complete cytological process of spermatogenesis remains unclear. In this study, we investigated the submicroscopic structure of total 23 t...
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ftfrontimediafig:oai:figshare.com:article/13552460 2023-05-15T18:15:41+02:00 Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf Yifan Liu Qinghua Liu Shihong Xu Yanfeng Wang Chengcheng Feng Chunyan Zhao Zongcheng Song Jun Li 2021-01-11T04:53:29Z https://doi.org/10.3389/fmars.2020.592880.s001 https://figshare.com/articles/figure/Image_1_A_Deep_Insight_of_Spermatogenesis_and_Hormone_Levels_of_Aqua-Cultured_Turbot_Scophthalmus_maximus_pdf/13552460 unknown doi:10.3389/fmars.2020.592880.s001 https://figshare.com/articles/figure/Image_1_A_Deep_Insight_of_Spermatogenesis_and_Hormone_Levels_of_Aqua-Cultured_Turbot_Scophthalmus_maximus_pdf/13552460 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering fish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus Image Figure 2021 ftfrontimediafig https://doi.org/10.3389/fmars.2020.592880.s001 2021-01-13T23:59:09Z Turbot (Scophthalmus maximus) is an important marine fish both in Europe and North China. Although there are plenty of studies on the reproduction of turbot, the complete cytological process of spermatogenesis remains unclear. In this study, we investigated the submicroscopic structure of total 23 types of male germ cells throughout the breeding season, with a relatively complete process of the primary spermatocytes. We found that the spermatid tail formed early at Spermatid II, and there were at least 16 spherical mitochondria in the spermatozoa. The hepatosomatic index (HSI) and gonadosomatic index (GSI) both peaked during the breeding season. Preliminary analysis showed that the vitality of mature sperm was negatively correlated with the proportion of sperm deformity. The serum 3,5,3′-triiodothyronine (T3), 5-hydroxytryptamine (5-HT), testosterone (T), 17α,20β-Dihydroxy-4-pregnen-3-one (17α,20β-DHP), and 17β-estradiol (E2) all increased during the maturity period, with the change of T content most noticeable. Whereas in the testis, an overall high level of 11-ketotestosterone (11-KT) was more remarkable. The expression and localization of androgen receptor (AR) mRNA showed that the AR was highly expressed at the stages of II (15 – 70 g), with a slight rebound at the mature stages [IV(2200 g) to V(2500 g)], whose change was ahead to the changes of T and 11-KT. Fluorescence in situ hybridization (FISH) analysis showed that the AR mainly distributed in but not limited to Sertoli cells. This study represents the most complete overview of the reproductive cycle and spermatogenesis of turbot, which provides an important reference for the reproduction research and the guidance of flatfish breeding. Still Image Scophthalmus maximus Turbot Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering fish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering fish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus Yifan Liu Qinghua Liu Shihong Xu Yanfeng Wang Chengcheng Feng Chunyan Zhao Zongcheng Song Jun Li Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering fish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus |
description |
Turbot (Scophthalmus maximus) is an important marine fish both in Europe and North China. Although there are plenty of studies on the reproduction of turbot, the complete cytological process of spermatogenesis remains unclear. In this study, we investigated the submicroscopic structure of total 23 types of male germ cells throughout the breeding season, with a relatively complete process of the primary spermatocytes. We found that the spermatid tail formed early at Spermatid II, and there were at least 16 spherical mitochondria in the spermatozoa. The hepatosomatic index (HSI) and gonadosomatic index (GSI) both peaked during the breeding season. Preliminary analysis showed that the vitality of mature sperm was negatively correlated with the proportion of sperm deformity. The serum 3,5,3′-triiodothyronine (T3), 5-hydroxytryptamine (5-HT), testosterone (T), 17α,20β-Dihydroxy-4-pregnen-3-one (17α,20β-DHP), and 17β-estradiol (E2) all increased during the maturity period, with the change of T content most noticeable. Whereas in the testis, an overall high level of 11-ketotestosterone (11-KT) was more remarkable. The expression and localization of androgen receptor (AR) mRNA showed that the AR was highly expressed at the stages of II (15 – 70 g), with a slight rebound at the mature stages [IV(2200 g) to V(2500 g)], whose change was ahead to the changes of T and 11-KT. Fluorescence in situ hybridization (FISH) analysis showed that the AR mainly distributed in but not limited to Sertoli cells. This study represents the most complete overview of the reproductive cycle and spermatogenesis of turbot, which provides an important reference for the reproduction research and the guidance of flatfish breeding. |
format |
Still Image |
author |
Yifan Liu Qinghua Liu Shihong Xu Yanfeng Wang Chengcheng Feng Chunyan Zhao Zongcheng Song Jun Li |
author_facet |
Yifan Liu Qinghua Liu Shihong Xu Yanfeng Wang Chengcheng Feng Chunyan Zhao Zongcheng Song Jun Li |
author_sort |
Yifan Liu |
title |
Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf |
title_short |
Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf |
title_full |
Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf |
title_fullStr |
Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf |
title_full_unstemmed |
Image_1_A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).pdf |
title_sort |
image_1_a deep insight of spermatogenesis and hormone levels of aqua-cultured turbot (scophthalmus maximus).pdf |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmars.2020.592880.s001 https://figshare.com/articles/figure/Image_1_A_Deep_Insight_of_Spermatogenesis_and_Hormone_Levels_of_Aqua-Cultured_Turbot_Scophthalmus_maximus_pdf/13552460 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
doi:10.3389/fmars.2020.592880.s001 https://figshare.com/articles/figure/Image_1_A_Deep_Insight_of_Spermatogenesis_and_Hormone_Levels_of_Aqua-Cultured_Turbot_Scophthalmus_maximus_pdf/13552460 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2020.592880.s001 |
_version_ |
1766188876088475648 |