Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif

Vitamin B1 (thiamin) is an essential coenzyme for all cells. Recent findings from experimental cell biology and genome surveys have shown that thiamin cycling by plankton is far more complex than was previously understood. Many plankton cells cannot produce thiamin (are auxotrophic) and obligately r...

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Main Authors: Christopher P. Suffridge, Luis M. Bolaños, Kristin Bergauer, Alexandra Z. Worden, Jeff Morré, Michael J. Behrenfeld, Stephen J. Giovannoni
Format: Still Image
Language:unknown
Published: 2020
Subjects:
Online Access:https://doi.org/10.3389/fmars.2020.606342.s001
https://figshare.com/articles/figure/Image_1_Exploring_Vitamin_B1_Cycling_and_Its_Connections_to_the_Microbial_Community_in_the_North_Atlantic_Ocean_tif/13474575
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spelling ftfrontimediafig:oai:figshare.com:article/13474575 2023-05-15T17:31:56+02:00 Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif Christopher P. Suffridge Luis M. Bolaños Kristin Bergauer Alexandra Z. Worden Jeff Morré Michael J. Behrenfeld Stephen J. Giovannoni 2020-12-22T05:16:26Z https://doi.org/10.3389/fmars.2020.606342.s001 https://figshare.com/articles/figure/Image_1_Exploring_Vitamin_B1_Cycling_and_Its_Connections_to_the_Microbial_Community_in_the_North_Atlantic_Ocean_tif/13474575 unknown doi:10.3389/fmars.2020.606342.s001 https://figshare.com/articles/figure/Image_1_Exploring_Vitamin_B1_Cycling_and_Its_Connections_to_the_Microbial_Community_in_the_North_Atlantic_Ocean_tif/13474575 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering thiamin B-vitamin bacterioplankton phytoplankton microbial interaction Image Figure 2020 ftfrontimediafig https://doi.org/10.3389/fmars.2020.606342.s001 2020-12-23T23:57:31Z Vitamin B1 (thiamin) is an essential coenzyme for all cells. Recent findings from experimental cell biology and genome surveys have shown that thiamin cycling by plankton is far more complex than was previously understood. Many plankton cells cannot produce thiamin (are auxotrophic) and obligately require an exogenous source of thiamin or one or more of 5 different thiamin-related compounds (TRCs). Despite this emerging evidence for the evolution among plankton of complex interactions related to thiamin, the influence of TRCs on plankton community structure and productivity are not understood. We report measurements of three dissolved TRCs 4-amino-5-aminomethyl-2-methylpyrimidine (AmMP), 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET), and 4-methyl-5-thiazoleethanol (HET) that have never before been assayed in seawater. Here we characterize them alongside other TRCs that were measured previously [thiamin and 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP)], in depth profiles from a latitudinal transect in the north Atlantic in March 2018. TRC concentrations ranged from femptomolar to picomolar. Surface depletion relative to a maximum near the bottom of the euphotic zone and low concentrations at deeper depths were consistent features. Our observations suggest that when bacterial abundance and production are low, TRC concentrations approach a steady state where TRC production and consumption terms are balanced. Standing stocks of TRCs also appear to be positively correlated with bacterial production. However, near the period of peak biomass in the accumulation phase of a bloom we observed an inverse relationship between TRCs and bacterial production, coincident with an increased abundance of Flavobacteria that comparative genomics indicates could be vitamin B1 auxotrophs. While these observations suggest that the dissolved pool of TRCs is often at steady state, with TRC production and consumption balanced, our data suggests that bloom induced shifts in microbial community structure and activity ... Still Image North Atlantic Frontiers: Figshare
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
thiamin
B-vitamin
bacterioplankton
phytoplankton
microbial interaction
spellingShingle Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
thiamin
B-vitamin
bacterioplankton
phytoplankton
microbial interaction
Christopher P. Suffridge
Luis M. Bolaños
Kristin Bergauer
Alexandra Z. Worden
Jeff Morré
Michael J. Behrenfeld
Stephen J. Giovannoni
Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif
topic_facet Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
thiamin
B-vitamin
bacterioplankton
phytoplankton
microbial interaction
description Vitamin B1 (thiamin) is an essential coenzyme for all cells. Recent findings from experimental cell biology and genome surveys have shown that thiamin cycling by plankton is far more complex than was previously understood. Many plankton cells cannot produce thiamin (are auxotrophic) and obligately require an exogenous source of thiamin or one or more of 5 different thiamin-related compounds (TRCs). Despite this emerging evidence for the evolution among plankton of complex interactions related to thiamin, the influence of TRCs on plankton community structure and productivity are not understood. We report measurements of three dissolved TRCs 4-amino-5-aminomethyl-2-methylpyrimidine (AmMP), 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET), and 4-methyl-5-thiazoleethanol (HET) that have never before been assayed in seawater. Here we characterize them alongside other TRCs that were measured previously [thiamin and 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP)], in depth profiles from a latitudinal transect in the north Atlantic in March 2018. TRC concentrations ranged from femptomolar to picomolar. Surface depletion relative to a maximum near the bottom of the euphotic zone and low concentrations at deeper depths were consistent features. Our observations suggest that when bacterial abundance and production are low, TRC concentrations approach a steady state where TRC production and consumption terms are balanced. Standing stocks of TRCs also appear to be positively correlated with bacterial production. However, near the period of peak biomass in the accumulation phase of a bloom we observed an inverse relationship between TRCs and bacterial production, coincident with an increased abundance of Flavobacteria that comparative genomics indicates could be vitamin B1 auxotrophs. While these observations suggest that the dissolved pool of TRCs is often at steady state, with TRC production and consumption balanced, our data suggests that bloom induced shifts in microbial community structure and activity ...
format Still Image
author Christopher P. Suffridge
Luis M. Bolaños
Kristin Bergauer
Alexandra Z. Worden
Jeff Morré
Michael J. Behrenfeld
Stephen J. Giovannoni
author_facet Christopher P. Suffridge
Luis M. Bolaños
Kristin Bergauer
Alexandra Z. Worden
Jeff Morré
Michael J. Behrenfeld
Stephen J. Giovannoni
author_sort Christopher P. Suffridge
title Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif
title_short Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif
title_full Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif
title_fullStr Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif
title_full_unstemmed Image_1_Exploring Vitamin B1 Cycling and Its Connections to the Microbial Community in the North Atlantic Ocean.tif
title_sort image_1_exploring vitamin b1 cycling and its connections to the microbial community in the north atlantic ocean.tif
publishDate 2020
url https://doi.org/10.3389/fmars.2020.606342.s001
https://figshare.com/articles/figure/Image_1_Exploring_Vitamin_B1_Cycling_and_Its_Connections_to_the_Microbial_Community_in_the_North_Atlantic_Ocean_tif/13474575
genre North Atlantic
genre_facet North Atlantic
op_relation doi:10.3389/fmars.2020.606342.s001
https://figshare.com/articles/figure/Image_1_Exploring_Vitamin_B1_Cycling_and_Its_Connections_to_the_Microbial_Community_in_the_North_Atlantic_Ocean_tif/13474575
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmars.2020.606342.s001
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