Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the sur...
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ftfrontimediafig:oai:figshare.com:article/13387148 2023-05-15T13:30:57+02:00 Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx Ignacio Gallardo-Carreño Mercedes Moreno-Paz Jacobo Aguirre Yolanda Blanco Eduardo Alonso-Pintado Isabelle Raymond-Bouchard Catherine Maggiori Luis A. Rivas Anna Engelbrektson Lyle Whyte Víctor Parro 2020-12-16T14:03:44Z https://doi.org/10.3389/fmicb.2020.590736.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_Multiplex_Immunosensor_for_Detecting_Perchlorate-Reducing_Bacteria_for_Environmental_Monitoring_and_Planetary_Exploration_docx/13387148 unknown doi:10.3389/fmicb.2020.590736.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_Multiplex_Immunosensor_for_Detecting_Perchlorate-Reducing_Bacteria_for_Environmental_Monitoring_and_Planetary_Exploration_docx/13387148 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology perchlorate perchlorate-reducing bacteria antibody microarrays biochip life detection planetary exploration Mars graph theory Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.590736.s001 2020-12-16T23:56:07Z Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. We have developed an antibody microarray with 20 polyclonal antibodies to detect perchlorate-reducing bacteria (PRB) strains and two crucial and highly conserved enzymes involved in perchlorate respiration: perchlorate reductase and chlorite dismutase. We determined the cross-reactivity, the working concentration, and the limit of detection of each antibody individually and in a multiplex format by Fluorescent Sandwich Microarray Immunoassay. Although most of them exhibited relatively high sensitivity and specificity, we applied a deconvolution method based on graph theory to discriminate between specific signals and cross-reactions from related microorganisms. We validated the system by analyzing multiple bacterial isolates, crude extracts from contaminated reactors and salt-rich natural samples from the high Arctic. The PRB detecting chip (PRBCHIP) allowed us to detect and classify environmental isolates as well as to detect similar strains by using crude extracts obtained from 0.5 g even from soils with low organic-matter levels (<10 3 cells/g of soil). Our results demonstrated that PRBCHIP is a valuable tool for sensitive and reliable detection of perchlorate-reducing bacteria for research purposes, environmental monitoring and planetary exploration. Dataset Antarc* Antarctic Arctic Frontiers: Figshare Arctic Antarctic The Antarctic |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology perchlorate perchlorate-reducing bacteria antibody microarrays biochip life detection planetary exploration Mars graph theory |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology perchlorate perchlorate-reducing bacteria antibody microarrays biochip life detection planetary exploration Mars graph theory Ignacio Gallardo-Carreño Mercedes Moreno-Paz Jacobo Aguirre Yolanda Blanco Eduardo Alonso-Pintado Isabelle Raymond-Bouchard Catherine Maggiori Luis A. Rivas Anna Engelbrektson Lyle Whyte Víctor Parro Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology perchlorate perchlorate-reducing bacteria antibody microarrays biochip life detection planetary exploration Mars graph theory |
description |
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. We have developed an antibody microarray with 20 polyclonal antibodies to detect perchlorate-reducing bacteria (PRB) strains and two crucial and highly conserved enzymes involved in perchlorate respiration: perchlorate reductase and chlorite dismutase. We determined the cross-reactivity, the working concentration, and the limit of detection of each antibody individually and in a multiplex format by Fluorescent Sandwich Microarray Immunoassay. Although most of them exhibited relatively high sensitivity and specificity, we applied a deconvolution method based on graph theory to discriminate between specific signals and cross-reactions from related microorganisms. We validated the system by analyzing multiple bacterial isolates, crude extracts from contaminated reactors and salt-rich natural samples from the high Arctic. The PRB detecting chip (PRBCHIP) allowed us to detect and classify environmental isolates as well as to detect similar strains by using crude extracts obtained from 0.5 g even from soils with low organic-matter levels (<10 3 cells/g of soil). Our results demonstrated that PRBCHIP is a valuable tool for sensitive and reliable detection of perchlorate-reducing bacteria for research purposes, environmental monitoring and planetary exploration. |
format |
Dataset |
author |
Ignacio Gallardo-Carreño Mercedes Moreno-Paz Jacobo Aguirre Yolanda Blanco Eduardo Alonso-Pintado Isabelle Raymond-Bouchard Catherine Maggiori Luis A. Rivas Anna Engelbrektson Lyle Whyte Víctor Parro |
author_facet |
Ignacio Gallardo-Carreño Mercedes Moreno-Paz Jacobo Aguirre Yolanda Blanco Eduardo Alonso-Pintado Isabelle Raymond-Bouchard Catherine Maggiori Luis A. Rivas Anna Engelbrektson Lyle Whyte Víctor Parro |
author_sort |
Ignacio Gallardo-Carreño |
title |
Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx |
title_short |
Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx |
title_full |
Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx |
title_fullStr |
Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx |
title_full_unstemmed |
Data_Sheet_1_A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.docx |
title_sort |
data_sheet_1_a multiplex immunosensor for detecting perchlorate-reducing bacteria for environmental monitoring and planetary exploration.docx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.590736.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_Multiplex_Immunosensor_for_Detecting_Perchlorate-Reducing_Bacteria_for_Environmental_Monitoring_and_Planetary_Exploration_docx/13387148 |
geographic |
Arctic Antarctic The Antarctic |
geographic_facet |
Arctic Antarctic The Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
doi:10.3389/fmicb.2020.590736.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_Multiplex_Immunosensor_for_Detecting_Perchlorate-Reducing_Bacteria_for_Environmental_Monitoring_and_Planetary_Exploration_docx/13387148 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.590736.s001 |
_version_ |
1766014465867776000 |