DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf
The absence of MHC class II antigen presentation and multiple pathogen recognition receptors in the Atlantic cod has not impaired its immune response however how underlying mechanisms have adapted remains largely unknown. In this study, ex vivo cod macrophages were challenged with various bacterial...
Main Authors: | , , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fimmu.2020.609456.s006 https://figshare.com/articles/dataset/DataSheet_6_Innovation_in_Nucleotide-Binding_Oligomerization-Like_Receptor_and_Toll-Like_Receptor_Sensing_Drives_the_Major_Histocompatibility_Complex-II_Free_Atlantic_Cod_Immune_System_pdf/13365071 |
id |
ftfrontimediafig:oai:figshare.com:article/13365071 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/13365071 2023-05-15T15:27:01+02:00 DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf Xingkun Jin Bernat Morro Ole K. Tørresen Visila Moiche Monica H. Solbakken Kjetill S. Jakobsen Sissel Jentoft Simon MacKenzie 2020-12-11T05:20:54Z https://doi.org/10.3389/fimmu.2020.609456.s006 https://figshare.com/articles/dataset/DataSheet_6_Innovation_in_Nucleotide-Binding_Oligomerization-Like_Receptor_and_Toll-Like_Receptor_Sensing_Drives_the_Major_Histocompatibility_Complex-II_Free_Atlantic_Cod_Immune_System_pdf/13365071 unknown doi:10.3389/fimmu.2020.609456.s006 https://figshare.com/articles/dataset/DataSheet_6_Innovation_in_Nucleotide-Binding_Oligomerization-Like_Receptor_and_Toll-Like_Receptor_Sensing_Drives_the_Major_Histocompatibility_Complex-II_Free_Atlantic_Cod_Immune_System_pdf/13365071 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cod immune response nucleotide-binding oligomerization-like receptor (NLR) macrophage Toll-like receptor (TLR) Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.609456.s006 2020-12-16T23:58:37Z The absence of MHC class II antigen presentation and multiple pathogen recognition receptors in the Atlantic cod has not impaired its immune response however how underlying mechanisms have adapted remains largely unknown. In this study, ex vivo cod macrophages were challenged with various bacterial and viral microbe-associated molecular patterns (MAMP) to identify major response pathways. Cytosolic MAMP-PRR pathways based upon the NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs) were identified as the critical response pathways. Our analyses suggest that internalization of exogenous ligands through scavenger receptors drives both pathways activating transcription factors like NF-kB (Nuclear factor-kappa B) and interferon regulatory factors (IRFs). Further, ligand-dependent differential expression of a unique TLR25 isoform and multiple NLR paralogues suggests (sub)neofunctionalization toward specific immune defensive strategies. Our results further demonstrate that the unique immune system of the Atlantic cod provides an unprecedented opportunity to explore the evolutionary history of PRR-based signaling in vertebrate immunity. Dataset atlantic cod Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cod immune response nucleotide-binding oligomerization-like receptor (NLR) macrophage Toll-like receptor (TLR) |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cod immune response nucleotide-binding oligomerization-like receptor (NLR) macrophage Toll-like receptor (TLR) Xingkun Jin Bernat Morro Ole K. Tørresen Visila Moiche Monica H. Solbakken Kjetill S. Jakobsen Sissel Jentoft Simon MacKenzie DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology cod immune response nucleotide-binding oligomerization-like receptor (NLR) macrophage Toll-like receptor (TLR) |
description |
The absence of MHC class II antigen presentation and multiple pathogen recognition receptors in the Atlantic cod has not impaired its immune response however how underlying mechanisms have adapted remains largely unknown. In this study, ex vivo cod macrophages were challenged with various bacterial and viral microbe-associated molecular patterns (MAMP) to identify major response pathways. Cytosolic MAMP-PRR pathways based upon the NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs) were identified as the critical response pathways. Our analyses suggest that internalization of exogenous ligands through scavenger receptors drives both pathways activating transcription factors like NF-kB (Nuclear factor-kappa B) and interferon regulatory factors (IRFs). Further, ligand-dependent differential expression of a unique TLR25 isoform and multiple NLR paralogues suggests (sub)neofunctionalization toward specific immune defensive strategies. Our results further demonstrate that the unique immune system of the Atlantic cod provides an unprecedented opportunity to explore the evolutionary history of PRR-based signaling in vertebrate immunity. |
format |
Dataset |
author |
Xingkun Jin Bernat Morro Ole K. Tørresen Visila Moiche Monica H. Solbakken Kjetill S. Jakobsen Sissel Jentoft Simon MacKenzie |
author_facet |
Xingkun Jin Bernat Morro Ole K. Tørresen Visila Moiche Monica H. Solbakken Kjetill S. Jakobsen Sissel Jentoft Simon MacKenzie |
author_sort |
Xingkun Jin |
title |
DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf |
title_short |
DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf |
title_full |
DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf |
title_fullStr |
DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf |
title_full_unstemmed |
DataSheet_6_Innovation in Nucleotide-Binding Oligomerization-Like Receptor and Toll-Like Receptor Sensing Drives the Major Histocompatibility Complex-II Free Atlantic Cod Immune System.pdf |
title_sort |
datasheet_6_innovation in nucleotide-binding oligomerization-like receptor and toll-like receptor sensing drives the major histocompatibility complex-ii free atlantic cod immune system.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.609456.s006 https://figshare.com/articles/dataset/DataSheet_6_Innovation_in_Nucleotide-Binding_Oligomerization-Like_Receptor_and_Toll-Like_Receptor_Sensing_Drives_the_Major_Histocompatibility_Complex-II_Free_Atlantic_Cod_Immune_System_pdf/13365071 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_relation |
doi:10.3389/fimmu.2020.609456.s006 https://figshare.com/articles/dataset/DataSheet_6_Innovation_in_Nucleotide-Binding_Oligomerization-Like_Receptor_and_Toll-Like_Receptor_Sensing_Drives_the_Major_Histocompatibility_Complex-II_Free_Atlantic_Cod_Immune_System_pdf/13365071 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.609456.s006 |
_version_ |
1766357481264513024 |