Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF
Early evidence suggests that DNA methylation can mediate phenotypic responses of marine calcifying species to ocean acidification (OA). Few studies, however, have explicitly studied DNA methylation in calcifying tissues through time. Here, we examined the phenotypic and molecular responses in the ex...
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Online Access: | https://doi.org/10.3389/fmars.2020.566419.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Ocean_Acidification_Induces_Subtle_Shifts_in_Gene_Expression_and_DNA_Methylation_in_Mantle_Tissue_of_the_Eastern_Oyster_Crassostrea_virginica_PDF/13232684 |
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ftfrontimediafig:oai:figshare.com:article/13232684 2023-05-15T17:51:07+02:00 Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF Alan M. Downey-Wall Louise P. Cameron Brett M. Ford Elise M. McNally Yaamini R. Venkataraman Steven B. Roberts Justin B. Ries Katie E. Lotterhos 2020-11-13T04:13:05Z https://doi.org/10.3389/fmars.2020.566419.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Ocean_Acidification_Induces_Subtle_Shifts_in_Gene_Expression_and_DNA_Methylation_in_Mantle_Tissue_of_the_Eastern_Oyster_Crassostrea_virginica_PDF/13232684 unknown doi:10.3389/fmars.2020.566419.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Ocean_Acidification_Induces_Subtle_Shifts_in_Gene_Expression_and_DNA_Methylation_in_Mantle_Tissue_of_the_Eastern_Oyster_Crassostrea_virginica_PDF/13232684 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering DNA methylation gene expression ocean acidification acclimatization Crassostrea virginica oyster extrapallial fluid Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmars.2020.566419.s001 2020-11-18T23:57:52Z Early evidence suggests that DNA methylation can mediate phenotypic responses of marine calcifying species to ocean acidification (OA). Few studies, however, have explicitly studied DNA methylation in calcifying tissues through time. Here, we examined the phenotypic and molecular responses in the extrapallial fluid and mantle (fluid and tissue at the calcification site) in adult eastern oyster (Crassostrea virginica) exposed to experimental OA over 80 days. Oysters were reared under three experimental pCO 2 treatments (“control,” 580 μatm; “moderate OA,” 1,000 μatm; “high OA,” 2,800 μatm) and sampled at 6 time points (24 h−80 days). We found that high OA initially induced an increase in the pH of the extrapallial fluid (pH EPF ) relative to the external seawater that peaked at day 9, but then diminished over time. Calcification rates were significantly lower in the high OA treatment compared to the other treatments. To explore how oysters regulate their extrapallial fluid, gene expression and DNA methylation were examined in the mantle-edge tissue of oysters from days 9 and 80 in the control and high OA treatments. Mantle tissue mounted a significant global molecular response (both in the transcriptome and methylome) to OA that shifted through time. Although we did not find individual genes that were significantly differentially expressed under OA, the pH EPF was significantly correlated with the eigengene expression of several co-expressed gene clusters. A small number of OA-induced differentially methylated loci were discovered, which corresponded with a weak association between OA-induced changes in genome-wide gene body DNA methylation and gene expression. Gene body methylation, however, was not significantly correlated with the eigengene expression of pH EPF -correlated gene clusters. These results suggest that OA induces a subtle response in a large number of genes in C. virginica, but also indicate that plasticity at the molecular level may be limited. Our study highlights the need to reassess our ... Dataset Ocean acidification Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering DNA methylation gene expression ocean acidification acclimatization Crassostrea virginica oyster extrapallial fluid |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering DNA methylation gene expression ocean acidification acclimatization Crassostrea virginica oyster extrapallial fluid Alan M. Downey-Wall Louise P. Cameron Brett M. Ford Elise M. McNally Yaamini R. Venkataraman Steven B. Roberts Justin B. Ries Katie E. Lotterhos Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering DNA methylation gene expression ocean acidification acclimatization Crassostrea virginica oyster extrapallial fluid |
description |
Early evidence suggests that DNA methylation can mediate phenotypic responses of marine calcifying species to ocean acidification (OA). Few studies, however, have explicitly studied DNA methylation in calcifying tissues through time. Here, we examined the phenotypic and molecular responses in the extrapallial fluid and mantle (fluid and tissue at the calcification site) in adult eastern oyster (Crassostrea virginica) exposed to experimental OA over 80 days. Oysters were reared under three experimental pCO 2 treatments (“control,” 580 μatm; “moderate OA,” 1,000 μatm; “high OA,” 2,800 μatm) and sampled at 6 time points (24 h−80 days). We found that high OA initially induced an increase in the pH of the extrapallial fluid (pH EPF ) relative to the external seawater that peaked at day 9, but then diminished over time. Calcification rates were significantly lower in the high OA treatment compared to the other treatments. To explore how oysters regulate their extrapallial fluid, gene expression and DNA methylation were examined in the mantle-edge tissue of oysters from days 9 and 80 in the control and high OA treatments. Mantle tissue mounted a significant global molecular response (both in the transcriptome and methylome) to OA that shifted through time. Although we did not find individual genes that were significantly differentially expressed under OA, the pH EPF was significantly correlated with the eigengene expression of several co-expressed gene clusters. A small number of OA-induced differentially methylated loci were discovered, which corresponded with a weak association between OA-induced changes in genome-wide gene body DNA methylation and gene expression. Gene body methylation, however, was not significantly correlated with the eigengene expression of pH EPF -correlated gene clusters. These results suggest that OA induces a subtle response in a large number of genes in C. virginica, but also indicate that plasticity at the molecular level may be limited. Our study highlights the need to reassess our ... |
format |
Dataset |
author |
Alan M. Downey-Wall Louise P. Cameron Brett M. Ford Elise M. McNally Yaamini R. Venkataraman Steven B. Roberts Justin B. Ries Katie E. Lotterhos |
author_facet |
Alan M. Downey-Wall Louise P. Cameron Brett M. Ford Elise M. McNally Yaamini R. Venkataraman Steven B. Roberts Justin B. Ries Katie E. Lotterhos |
author_sort |
Alan M. Downey-Wall |
title |
Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF |
title_short |
Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF |
title_full |
Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF |
title_fullStr |
Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF |
title_full_unstemmed |
Data_Sheet_1_Ocean Acidification Induces Subtle Shifts in Gene Expression and DNA Methylation in Mantle Tissue of the Eastern Oyster (Crassostrea virginica).PDF |
title_sort |
data_sheet_1_ocean acidification induces subtle shifts in gene expression and dna methylation in mantle tissue of the eastern oyster (crassostrea virginica).pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmars.2020.566419.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Ocean_Acidification_Induces_Subtle_Shifts_in_Gene_Expression_and_DNA_Methylation_in_Mantle_Tissue_of_the_Eastern_Oyster_Crassostrea_virginica_PDF/13232684 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.3389/fmars.2020.566419.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Ocean_Acidification_Induces_Subtle_Shifts_in_Gene_Expression_and_DNA_Methylation_in_Mantle_Tissue_of_the_Eastern_Oyster_Crassostrea_virginica_PDF/13232684 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2020.566419.s001 |
_version_ |
1766158148223107072 |