Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx
Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as m...
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ftfrontimediafig:oai:figshare.com:article/13219241 2023-05-15T15:30:42+02:00 Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian 2020-11-11T04:24:00Z https://doi.org/10.3389/fimmu.2020.587931.s005 https://figshare.com/articles/dataset/Table_5_Characterization_of_miRNAs_in_Extracellular_Vesicles_Released_From_Atlantic_Salmon_Monocyte-Like_and_Macrophage-Like_Cells_xlsx/13219241 unknown doi:10.3389/fimmu.2020.587931.s005 https://figshare.com/articles/dataset/Table_5_Characterization_of_miRNAs_in_Extracellular_Vesicles_Released_From_Atlantic_Salmon_Monocyte-Like_and_Macrophage-Like_Cells_xlsx/13219241 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage head kidney culture Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.587931.s005 2020-11-11T23:55:28Z Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as macrophages. Our previous research revealed that Atlantic salmon head kidney leukocytes (HKLs) change their morphology, phagocytic ability and miRNA profile from primarily “monocyte-like” at Day 1 to primarily “macrophage-like” at Day 5 of culture. Therefore, we aimed to characterize the miRNA cargo packaged in EVs released from these two cell populations. We successfully isolated EVs from Atlantic salmon HKL culture supernatants using the established Vn96 peptide-based pull-down. Isolation was validated using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. RNA-sequencing identified 19 differentially enriched (DE) miRNAs packaged in Day 1 versus Day 5 EVs. Several of the highly abundant miRNAs, including those that were DE (e.g. ssa-miR-146a, ssa-miR-155 and ssa-miR-731), were previously identified as DE in HKLs and are associated with macrophage differentiation and immune response in other species. Interestingly, the abundance relative of the miRNAs in EVs, including the most abundant miRNA (ssa-miR-125b), was different than the miRNA abundance in HKLs, indicating selective packaging of miRNAs in EVs. Further study of the miRNA cargo in EVs derived from fish immune cells will be an important next step in identifying EV biomarkers useful for evaluating immune cell function, fish health, or response to disease. Dataset Atlantic salmon Frontiers: Figshare |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage head kidney culture |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage head kidney culture Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology extracellular vesicles microRNA Atlantic salmon RNA-sequencing RNA-seq macrophage head kidney culture |
description |
Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as macrophages. Our previous research revealed that Atlantic salmon head kidney leukocytes (HKLs) change their morphology, phagocytic ability and miRNA profile from primarily “monocyte-like” at Day 1 to primarily “macrophage-like” at Day 5 of culture. Therefore, we aimed to characterize the miRNA cargo packaged in EVs released from these two cell populations. We successfully isolated EVs from Atlantic salmon HKL culture supernatants using the established Vn96 peptide-based pull-down. Isolation was validated using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. RNA-sequencing identified 19 differentially enriched (DE) miRNAs packaged in Day 1 versus Day 5 EVs. Several of the highly abundant miRNAs, including those that were DE (e.g. ssa-miR-146a, ssa-miR-155 and ssa-miR-731), were previously identified as DE in HKLs and are associated with macrophage differentiation and immune response in other species. Interestingly, the abundance relative of the miRNAs in EVs, including the most abundant miRNA (ssa-miR-125b), was different than the miRNA abundance in HKLs, indicating selective packaging of miRNAs in EVs. Further study of the miRNA cargo in EVs derived from fish immune cells will be an important next step in identifying EV biomarkers useful for evaluating immune cell function, fish health, or response to disease. |
format |
Dataset |
author |
Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian |
author_facet |
Nicole C. Smith Gabriel Wajnberg Simi Chacko Nardos T. Woldemariam Jacynthe Lacroix Nicolas Crapoulet D. Craig Ayre Stephen M. Lewis Matthew L. Rise Rune Andreassen Sherri L. Christian |
author_sort |
Nicole C. Smith |
title |
Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx |
title_short |
Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx |
title_full |
Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx |
title_fullStr |
Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx |
title_full_unstemmed |
Table_5_Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.xlsx |
title_sort |
table_5_characterization of mirnas in extracellular vesicles released from atlantic salmon monocyte-like and macrophage-like cells.xlsx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.587931.s005 https://figshare.com/articles/dataset/Table_5_Characterization_of_miRNAs_in_Extracellular_Vesicles_Released_From_Atlantic_Salmon_Monocyte-Like_and_Macrophage-Like_Cells_xlsx/13219241 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.3389/fimmu.2020.587931.s005 https://figshare.com/articles/dataset/Table_5_Characterization_of_miRNAs_in_Extracellular_Vesicles_Released_From_Atlantic_Salmon_Monocyte-Like_and_Macrophage-Like_Cells_xlsx/13219241 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.587931.s005 |
_version_ |
1766361153732083712 |