Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF
With the expansion of the aquaculture industry in the last two decades, there has been a large increase in the use of plant ingredients in aquafeeds, which has created new challenges in fish growth, health and welfare. Fish muscle growth is an important trait that is strongly affected by diet, but o...
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ftfrontimediafig:oai:figshare.com:article/13127567 2023-05-15T15:31:30+02:00 Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF Anusha K. S. Dhanasiri Amritha Johny Xi Xue Gerd M. Berge Andre S. Bogevik Matthew L. Rise Christiane K. Fæste Jorge M. O. Fernandes 2020-10-22T05:11:40Z https://doi.org/10.3389/fgene.2020.575237.s002 https://figshare.com/articles/dataset/Data_Sheet_2_Plant-Based_Diets_Induce_Transcriptomic_Changes_in_Muscle_of_Zebrafish_and_Atlantic_Salmon_PDF/13127567 unknown doi:10.3389/fgene.2020.575237.s002 https://figshare.com/articles/dataset/Data_Sheet_2_Plant-Based_Diets_Induce_Transcriptomic_Changes_in_Muscle_of_Zebrafish_and_Atlantic_Salmon_PDF/13127567 CC BY 4.0 CC-BY Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy plant-based proteins fast muscle pea protein concentrate soy protein concentrate wheat gluten gene expression Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fgene.2020.575237.s002 2020-10-28T23:58:08Z With the expansion of the aquaculture industry in the last two decades, there has been a large increase in the use of plant ingredients in aquafeeds, which has created new challenges in fish growth, health and welfare. Fish muscle growth is an important trait that is strongly affected by diet, but our knowledge on the effect of plant protein-based diets on global gene expression in muscle is still scant. The present study evaluated nutrigenomic effects of the inclusion of proteins from pea, soy and wheat into aquafeeds, compared to a control diet with fishmeal as the main protein source using the zebrafish model by RNA-seq; these results were extended to an important aquaculture species by analyzing selected differentially expressed genes identified in the zebrafish model on on-growing Atlantic salmon fed with equivalent plant protein-based diets. Expression of selected Atlantic salmon paralogues of the zebrafish homologs was analyzed using paralogue-specific qPCR assays. Global gene expression changes in muscle of zebrafish fed with plant-based diets were moderate, with the highest changes observed in the soy diet-fed fish, and no change for the pea diet-fed fish compared to the control diet. Among the differentially expressed genes were mylpfb, hsp90aa1.1, col2a1a, and odc1, which are important in regulating muscle growth, maintaining muscle structure and function, and muscle tissue homeostasis. Furthermore, those genes and their paralogues were differentially expressed in Atlantic salmon fed with the equivalent percentage of soy or wheat protein containing diets. Some of these genes were similarly regulated in both species while others showed species-specific regulation. The present study expands our understanding on the molecular effects of plant ingredients in fish muscle. Ultimately, the knowledge gained would be of importance for the improved formulation of sustainable plant-based diets for the aquaculture industry. Dataset Atlantic salmon Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy plant-based proteins fast muscle pea protein concentrate soy protein concentrate wheat gluten gene expression |
spellingShingle |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy plant-based proteins fast muscle pea protein concentrate soy protein concentrate wheat gluten gene expression Anusha K. S. Dhanasiri Amritha Johny Xi Xue Gerd M. Berge Andre S. Bogevik Matthew L. Rise Christiane K. Fæste Jorge M. O. Fernandes Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF |
topic_facet |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy plant-based proteins fast muscle pea protein concentrate soy protein concentrate wheat gluten gene expression |
description |
With the expansion of the aquaculture industry in the last two decades, there has been a large increase in the use of plant ingredients in aquafeeds, which has created new challenges in fish growth, health and welfare. Fish muscle growth is an important trait that is strongly affected by diet, but our knowledge on the effect of plant protein-based diets on global gene expression in muscle is still scant. The present study evaluated nutrigenomic effects of the inclusion of proteins from pea, soy and wheat into aquafeeds, compared to a control diet with fishmeal as the main protein source using the zebrafish model by RNA-seq; these results were extended to an important aquaculture species by analyzing selected differentially expressed genes identified in the zebrafish model on on-growing Atlantic salmon fed with equivalent plant protein-based diets. Expression of selected Atlantic salmon paralogues of the zebrafish homologs was analyzed using paralogue-specific qPCR assays. Global gene expression changes in muscle of zebrafish fed with plant-based diets were moderate, with the highest changes observed in the soy diet-fed fish, and no change for the pea diet-fed fish compared to the control diet. Among the differentially expressed genes were mylpfb, hsp90aa1.1, col2a1a, and odc1, which are important in regulating muscle growth, maintaining muscle structure and function, and muscle tissue homeostasis. Furthermore, those genes and their paralogues were differentially expressed in Atlantic salmon fed with the equivalent percentage of soy or wheat protein containing diets. Some of these genes were similarly regulated in both species while others showed species-specific regulation. The present study expands our understanding on the molecular effects of plant ingredients in fish muscle. Ultimately, the knowledge gained would be of importance for the improved formulation of sustainable plant-based diets for the aquaculture industry. |
format |
Dataset |
author |
Anusha K. S. Dhanasiri Amritha Johny Xi Xue Gerd M. Berge Andre S. Bogevik Matthew L. Rise Christiane K. Fæste Jorge M. O. Fernandes |
author_facet |
Anusha K. S. Dhanasiri Amritha Johny Xi Xue Gerd M. Berge Andre S. Bogevik Matthew L. Rise Christiane K. Fæste Jorge M. O. Fernandes |
author_sort |
Anusha K. S. Dhanasiri |
title |
Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF |
title_short |
Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF |
title_full |
Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF |
title_fullStr |
Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF |
title_full_unstemmed |
Data_Sheet_2_Plant-Based Diets Induce Transcriptomic Changes in Muscle of Zebrafish and Atlantic Salmon.PDF |
title_sort |
data_sheet_2_plant-based diets induce transcriptomic changes in muscle of zebrafish and atlantic salmon.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fgene.2020.575237.s002 https://figshare.com/articles/dataset/Data_Sheet_2_Plant-Based_Diets_Induce_Transcriptomic_Changes_in_Muscle_of_Zebrafish_and_Atlantic_Salmon_PDF/13127567 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.3389/fgene.2020.575237.s002 https://figshare.com/articles/dataset/Data_Sheet_2_Plant-Based_Diets_Induce_Transcriptomic_Changes_in_Muscle_of_Zebrafish_and_Atlantic_Salmon_PDF/13127567 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fgene.2020.575237.s002 |
_version_ |
1766362012703522816 |