Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf
The Atlantic cod’s unusual immune system, entirely lacking the Major Histocompatibility class II pathway, has prompted intriguing questions about what mechanisms are used to combat bacterial infections and how immunological memory is generated. By single-cell RNA sequencing we here report an in-dept...
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ftfrontimediafig:oai:figshare.com:article/13071593 2023-05-15T15:26:51+02:00 Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao 2020-10-09T04:49:03Z https://doi.org/10.3389/fimmu.2020.559555.s002 https://figshare.com/articles/dataset/Table_2_Single-Cell_Transcriptome_Profiling_of_Immune_Cell_Repertoire_of_the_Atlantic_Cod_Which_Naturally_Lacks_the_Major_Histocompatibility_Class_II_System_pdf/13071593 unknown doi:10.3389/fimmu.2020.559555.s002 https://figshare.com/articles/dataset/Table_2_Single-Cell_Transcriptome_Profiling_of_Immune_Cell_Repertoire_of_the_Atlantic_Cod_Which_Naturally_Lacks_the_Major_Histocompatibility_Class_II_System_pdf/13071593 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.559555.s002 2020-10-14T22:55:37Z The Atlantic cod’s unusual immune system, entirely lacking the Major Histocompatibility class II pathway, has prompted intriguing questions about what mechanisms are used to combat bacterial infections and how immunological memory is generated. By single-cell RNA sequencing we here report an in-depth characterisation of cell types found in immune tissues, the spleen and peripheral blood leukocytes of Atlantic cod. Unbiased transcriptional clustering revealed eleven distinct immune cell signatures. Resolution at the single cell level enabled characterisation of the major cell subsets including the cytotoxic T cells, B cells, erythrocytes, thrombocytes, neutrophils, and macrophages. Additionally, to our knowledge we are the first to uncover cell subsets in Atlantic cod which may represent dendritic cells, natural killer-like cells, and a population of cytotoxic cells expressing GATA-3, a master transcription factor of T helper 2 cells. We further identify putative gene markers for each cluster and describe the relative proportions of each cell type in the spleen and peripheral blood leukocytes. Of the major haematopoietic cell populations, the lymphocytes make up 55 and 68% of the spleen and peripheral blood leukocytes respectively, while the myeloid cells make up 45 and 32%. By single-cell analysis, this study provides the most detailed molecular and cellular characterisation of the immune system of the Atlantic cod so far. Dataset atlantic cod Gadus morhua Frontiers: Figshare Gata ENVELOPE(-19.702,-19.702,63.540,63.540) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker |
description |
The Atlantic cod’s unusual immune system, entirely lacking the Major Histocompatibility class II pathway, has prompted intriguing questions about what mechanisms are used to combat bacterial infections and how immunological memory is generated. By single-cell RNA sequencing we here report an in-depth characterisation of cell types found in immune tissues, the spleen and peripheral blood leukocytes of Atlantic cod. Unbiased transcriptional clustering revealed eleven distinct immune cell signatures. Resolution at the single cell level enabled characterisation of the major cell subsets including the cytotoxic T cells, B cells, erythrocytes, thrombocytes, neutrophils, and macrophages. Additionally, to our knowledge we are the first to uncover cell subsets in Atlantic cod which may represent dendritic cells, natural killer-like cells, and a population of cytotoxic cells expressing GATA-3, a master transcription factor of T helper 2 cells. We further identify putative gene markers for each cluster and describe the relative proportions of each cell type in the spleen and peripheral blood leukocytes. Of the major haematopoietic cell populations, the lymphocytes make up 55 and 68% of the spleen and peripheral blood leukocytes respectively, while the myeloid cells make up 45 and 32%. By single-cell analysis, this study provides the most detailed molecular and cellular characterisation of the immune system of the Atlantic cod so far. |
format |
Dataset |
author |
Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao |
author_facet |
Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao |
author_sort |
Naomi Croft Guslund |
title |
Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf |
title_short |
Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf |
title_full |
Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf |
title_fullStr |
Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf |
title_full_unstemmed |
Table_2_Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System.pdf |
title_sort |
table_2_single-cell transcriptome profiling of immune cell repertoire of the atlantic cod which naturally lacks the major histocompatibility class ii system.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.559555.s002 https://figshare.com/articles/dataset/Table_2_Single-Cell_Transcriptome_Profiling_of_Immune_Cell_Repertoire_of_the_Atlantic_Cod_Which_Naturally_Lacks_the_Major_Histocompatibility_Class_II_System_pdf/13071593 |
long_lat |
ENVELOPE(-19.702,-19.702,63.540,63.540) |
geographic |
Gata |
geographic_facet |
Gata |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
doi:10.3389/fimmu.2020.559555.s002 https://figshare.com/articles/dataset/Table_2_Single-Cell_Transcriptome_Profiling_of_Immune_Cell_Repertoire_of_the_Atlantic_Cod_Which_Naturally_Lacks_the_Major_Histocompatibility_Class_II_System_pdf/13071593 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.559555.s002 |
_version_ |
1766357322778542080 |