Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx
The onset of symbiosis and the early development of most broadcast spawning corals play pivotal roles in recruitment success, yet these critical early stages are threatened by multiple stressors. However, molecular mechanisms governing these critical processes under ocean warming and acidification a...
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Online Access: | https://doi.org/10.3389/fmicb.2020.532447.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Ocean_Warming_and_Acidification_on_Symbiosis_Establishment_and_Gene_Expression_Profiles_in_Recruits_of_Reef_Coral_Acropora_intermedia_docx/13026395 |
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ftfrontimediafig:oai:figshare.com:article/13026395 2023-05-15T17:52:04+02:00 Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx Youfang Sun Lei Jiang Sanqiang Gong Minglan Guo Xiangcheng Yuan Guowei Zhou Xinming Lei Yuyang Zhang Tao Yuan Jiansheng Lian Peiyuan Qian Hui Huang 2020-09-30T08:41:20Z https://doi.org/10.3389/fmicb.2020.532447.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Ocean_Warming_and_Acidification_on_Symbiosis_Establishment_and_Gene_Expression_Profiles_in_Recruits_of_Reef_Coral_Acropora_intermedia_docx/13026395 unknown doi:10.3389/fmicb.2020.532447.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Ocean_Warming_and_Acidification_on_Symbiosis_Establishment_and_Gene_Expression_Profiles_in_Recruits_of_Reef_Coral_Acropora_intermedia_docx/13026395 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Symbiodiniaceae symbiosis juvenile Acropora intermedia ocean warming ocean acidification transcriptomics Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.532447.s001 2020-09-30T22:55:05Z The onset of symbiosis and the early development of most broadcast spawning corals play pivotal roles in recruitment success, yet these critical early stages are threatened by multiple stressors. However, molecular mechanisms governing these critical processes under ocean warming and acidification are still poorly understood. The present study investigated the interactive impact of elevated temperature (∼28.0°C and ∼30.5°C) and partial pressure of carbon dioxide (pCO 2 ) (∼600 and ∼1,200 μatm) on early development and the gene expression patterns in juvenile Acropora intermedia over 33 days. The results showed that coral survival was >89% and was unaffected by high temperature, pCO 2 , or the combined treatment. Notably, high temperature completely arrested successful symbiosis establishment and the budding process, whereas acidification had a negligible effect. Moreover, there was a positive exponential relationship between symbiosis establishment and budding rates (y = 0.0004e 6.43x , R = 0.72, P < 0.0001), which indicated the importance of symbiosis in fueling asexual budding. Compared with corals at the control temperature (28°C), those under elevated temperature preferentially harbored Durusdinium spp., despite unsuccessful symbiosis establishment. In addition, compared to the control, 351 and 153 differentially expressed genes were detected in the symbiont and coral host in response to experimental conditions, respectively. In coral host, some genes involved in nutrient transportation and tissue fluorescence were affected by high temperature. In the symbionts, a suite of genes related to cell growth, ribosomal proteins, photosynthesis, and energy production was downregulated under high temperatures, which may have severely hampered successful cell proliferation of the endosymbionts and explains the failure of symbiosis establishment. Therefore, our results suggest that the responses of symbionts to future ocean conditions could play a vital role in shaping successful symbiosis in juvenile coral. Dataset Ocean acidification Frontiers: Figshare |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Symbiodiniaceae symbiosis juvenile Acropora intermedia ocean warming ocean acidification transcriptomics |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Symbiodiniaceae symbiosis juvenile Acropora intermedia ocean warming ocean acidification transcriptomics Youfang Sun Lei Jiang Sanqiang Gong Minglan Guo Xiangcheng Yuan Guowei Zhou Xinming Lei Yuyang Zhang Tao Yuan Jiansheng Lian Peiyuan Qian Hui Huang Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Symbiodiniaceae symbiosis juvenile Acropora intermedia ocean warming ocean acidification transcriptomics |
description |
The onset of symbiosis and the early development of most broadcast spawning corals play pivotal roles in recruitment success, yet these critical early stages are threatened by multiple stressors. However, molecular mechanisms governing these critical processes under ocean warming and acidification are still poorly understood. The present study investigated the interactive impact of elevated temperature (∼28.0°C and ∼30.5°C) and partial pressure of carbon dioxide (pCO 2 ) (∼600 and ∼1,200 μatm) on early development and the gene expression patterns in juvenile Acropora intermedia over 33 days. The results showed that coral survival was >89% and was unaffected by high temperature, pCO 2 , or the combined treatment. Notably, high temperature completely arrested successful symbiosis establishment and the budding process, whereas acidification had a negligible effect. Moreover, there was a positive exponential relationship between symbiosis establishment and budding rates (y = 0.0004e 6.43x , R = 0.72, P < 0.0001), which indicated the importance of symbiosis in fueling asexual budding. Compared with corals at the control temperature (28°C), those under elevated temperature preferentially harbored Durusdinium spp., despite unsuccessful symbiosis establishment. In addition, compared to the control, 351 and 153 differentially expressed genes were detected in the symbiont and coral host in response to experimental conditions, respectively. In coral host, some genes involved in nutrient transportation and tissue fluorescence were affected by high temperature. In the symbionts, a suite of genes related to cell growth, ribosomal proteins, photosynthesis, and energy production was downregulated under high temperatures, which may have severely hampered successful cell proliferation of the endosymbionts and explains the failure of symbiosis establishment. Therefore, our results suggest that the responses of symbionts to future ocean conditions could play a vital role in shaping successful symbiosis in juvenile coral. |
format |
Dataset |
author |
Youfang Sun Lei Jiang Sanqiang Gong Minglan Guo Xiangcheng Yuan Guowei Zhou Xinming Lei Yuyang Zhang Tao Yuan Jiansheng Lian Peiyuan Qian Hui Huang |
author_facet |
Youfang Sun Lei Jiang Sanqiang Gong Minglan Guo Xiangcheng Yuan Guowei Zhou Xinming Lei Yuyang Zhang Tao Yuan Jiansheng Lian Peiyuan Qian Hui Huang |
author_sort |
Youfang Sun |
title |
Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx |
title_short |
Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx |
title_full |
Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx |
title_fullStr |
Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx |
title_full_unstemmed |
Data_Sheet_1_Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral Acropora intermedia.docx |
title_sort |
data_sheet_1_impact of ocean warming and acidification on symbiosis establishment and gene expression profiles in recruits of reef coral acropora intermedia.docx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.532447.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Ocean_Warming_and_Acidification_on_Symbiosis_Establishment_and_Gene_Expression_Profiles_in_Recruits_of_Reef_Coral_Acropora_intermedia_docx/13026395 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.3389/fmicb.2020.532447.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Ocean_Warming_and_Acidification_on_Symbiosis_Establishment_and_Gene_Expression_Profiles_in_Recruits_of_Reef_Coral_Acropora_intermedia_docx/13026395 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.532447.s001 |
_version_ |
1766159393083097088 |