Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx
Glycerol, a compatible solute, has previously been found to act as an osmoprotectant in some marine Chlamydomonas species and several species of Dunaliella from hypersaline ponds. Recently, Chlamydomonas reinhardtii and Dunaliella salina were shown to make glycerol with an unusual bidomain enzyme, w...
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ftfrontimediafig:oai:figshare.com:article/12831773 2023-05-15T14:04:09+02:00 Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx James A. Raymond Rachael Morgan-Kiss Sarah Stahl-Rommel 2020-08-20T04:51:48Z https://doi.org/10.3389/fpls.2020.01259.s002 https://figshare.com/articles/dataset/Table_1_Glycerol_Is_an_Osmoprotectant_in_Two_Antarctic_Chlamydomonas_Species_From_an_Ice-Covered_Saline_Lake_and_Is_Synthesized_by_an_Unusual_Bidomain_Enzyme_docx/12831773 unknown doi:10.3389/fpls.2020.01259.s002 https://figshare.com/articles/dataset/Table_1_Glycerol_Is_an_Osmoprotectant_in_Two_Antarctic_Chlamydomonas_Species_From_an_Ice-Covered_Saline_Lake_and_Is_Synthesized_by_an_Unusual_Bidomain_Enzyme_docx/12831773 Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Chlamydomonas glycerol synthesis phosphoserine phosphatase glycerol-3-phosphate dehydrogenase Antarctica Lake Bonney Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fpls.2020.01259.s002 2020-08-26T22:56:26Z Glycerol, a compatible solute, has previously been found to act as an osmoprotectant in some marine Chlamydomonas species and several species of Dunaliella from hypersaline ponds. Recently, Chlamydomonas reinhardtii and Dunaliella salina were shown to make glycerol with an unusual bidomain enzyme, which appears to be unique to algae, that contains a phosphoserine phosphatase and glycerol-3-phosphate dehydrogenase. Here we report that two psychrophilic species of Chlamydomonas (C. spp. UWO241 and ICE-MDV) from Lake Bonney, Antarctica also produce high levels of glycerol to survive in the lake’s saline waters. Glycerol concentration increased linearly with salinity and at 1.3 M NaCl, exceeded 400 mM in C. sp. UWO241, the more salt-tolerant strain. We also show that both species expressed several isoforms of the bidomain enzyme. An analysis of one of the isoforms of C. sp. UWO241 showed that it was strongly upregulated by NaCl and is thus the likely source of glycerol. These results reveal another adaptation of the Lake Bonney Chlamydomonas species that allow them to survive in an extreme polar environment. Dataset Antarc* Antarctic Antarctica Frontiers: Figshare Antarctic Bonney ENVELOPE(162.417,162.417,-77.717,-77.717) Lake Bonney ENVELOPE(-25.588,-25.588,-80.361,-80.361) Saline Lake ENVELOPE(-111.528,-111.528,57.081,57.081) |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Chlamydomonas glycerol synthesis phosphoserine phosphatase glycerol-3-phosphate dehydrogenase Antarctica Lake Bonney |
spellingShingle |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Chlamydomonas glycerol synthesis phosphoserine phosphatase glycerol-3-phosphate dehydrogenase Antarctica Lake Bonney James A. Raymond Rachael Morgan-Kiss Sarah Stahl-Rommel Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx |
topic_facet |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Chlamydomonas glycerol synthesis phosphoserine phosphatase glycerol-3-phosphate dehydrogenase Antarctica Lake Bonney |
description |
Glycerol, a compatible solute, has previously been found to act as an osmoprotectant in some marine Chlamydomonas species and several species of Dunaliella from hypersaline ponds. Recently, Chlamydomonas reinhardtii and Dunaliella salina were shown to make glycerol with an unusual bidomain enzyme, which appears to be unique to algae, that contains a phosphoserine phosphatase and glycerol-3-phosphate dehydrogenase. Here we report that two psychrophilic species of Chlamydomonas (C. spp. UWO241 and ICE-MDV) from Lake Bonney, Antarctica also produce high levels of glycerol to survive in the lake’s saline waters. Glycerol concentration increased linearly with salinity and at 1.3 M NaCl, exceeded 400 mM in C. sp. UWO241, the more salt-tolerant strain. We also show that both species expressed several isoforms of the bidomain enzyme. An analysis of one of the isoforms of C. sp. UWO241 showed that it was strongly upregulated by NaCl and is thus the likely source of glycerol. These results reveal another adaptation of the Lake Bonney Chlamydomonas species that allow them to survive in an extreme polar environment. |
format |
Dataset |
author |
James A. Raymond Rachael Morgan-Kiss Sarah Stahl-Rommel |
author_facet |
James A. Raymond Rachael Morgan-Kiss Sarah Stahl-Rommel |
author_sort |
James A. Raymond |
title |
Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx |
title_short |
Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx |
title_full |
Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx |
title_fullStr |
Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx |
title_full_unstemmed |
Table_1_Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme.docx |
title_sort |
table_1_glycerol is an osmoprotectant in two antarctic chlamydomonas species from an ice-covered saline lake and is synthesized by an unusual bidomain enzyme.docx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fpls.2020.01259.s002 https://figshare.com/articles/dataset/Table_1_Glycerol_Is_an_Osmoprotectant_in_Two_Antarctic_Chlamydomonas_Species_From_an_Ice-Covered_Saline_Lake_and_Is_Synthesized_by_an_Unusual_Bidomain_Enzyme_docx/12831773 |
long_lat |
ENVELOPE(162.417,162.417,-77.717,-77.717) ENVELOPE(-25.588,-25.588,-80.361,-80.361) ENVELOPE(-111.528,-111.528,57.081,57.081) |
geographic |
Antarctic Bonney Lake Bonney Saline Lake |
geographic_facet |
Antarctic Bonney Lake Bonney Saline Lake |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.3389/fpls.2020.01259.s002 https://figshare.com/articles/dataset/Table_1_Glycerol_Is_an_Osmoprotectant_in_Two_Antarctic_Chlamydomonas_Species_From_an_Ice-Covered_Saline_Lake_and_Is_Synthesized_by_an_Unusual_Bidomain_Enzyme_docx/12831773 |
op_doi |
https://doi.org/10.3389/fpls.2020.01259.s002 |
_version_ |
1766275166796513280 |