Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX
Soil microbiomes within oligotrophic cold deserts are extraordinarily diverse. Increasingly, oligotrophic sites with low levels of phototrophic primary producers are reported, leading researchers to question their carbon and energy sources. A novel microbial carbon fixation process termed atmospheri...
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Online Access: | https://doi.org/10.3389/fmicb.2020.01936.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Soil_Microbiomes_With_the_Genetic_Capacity_for_Atmospheric_Chemosynthesis_Are_Widespread_Across_the_Poles_and_Are_Associated_With_Moisture_Carbon_and_Nitrogen_Limitation_DOCX/12794903 |
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ftfrontimediafig:oai:figshare.com:article/12794903 2023-05-15T14:04:09+02:00 Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX Angelique E. Ray Eden Zhang Aleks Terauds Mukan Ji Weidong Kong Belinda C. Ferrari 2020-08-12T15:02:09Z https://doi.org/10.3389/fmicb.2020.01936.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Soil_Microbiomes_With_the_Genetic_Capacity_for_Atmospheric_Chemosynthesis_Are_Widespread_Across_the_Poles_and_Are_Associated_With_Moisture_Carbon_and_Nitrogen_Limitation_DOCX/12794903 unknown doi:10.3389/fmicb.2020.01936.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Soil_Microbiomes_With_the_Genetic_Capacity_for_Atmospheric_Chemosynthesis_Are_Widespread_Across_the_Poles_and_Are_Associated_With_Moisture_Carbon_and_Nitrogen_Limitation_DOCX/12794903 Microbiology Microbial Genetics Microbial Ecology Mycology carbon fixation atmospheric chemosynthesis trace gases photosynthesis environmental drivers quantitative PCR Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.01936.s001 2020-08-12T22:53:54Z Soil microbiomes within oligotrophic cold deserts are extraordinarily diverse. Increasingly, oligotrophic sites with low levels of phototrophic primary producers are reported, leading researchers to question their carbon and energy sources. A novel microbial carbon fixation process termed atmospheric chemosynthesis recently filled this gap as it was shown to be supporting primary production at two Eastern Antarctic deserts. Atmospheric chemosynthesis uses energy liberated from the oxidation of atmospheric hydrogen to drive the Calvin-Benson-Bassham (CBB) cycle through a new chemotrophic form of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), designated IE. Here, we propose that the genetic determinants of this process; RuBisCO type IE (rbcL1E) and high affinity group 1h-[NiFe]-hydrogenase (hhyL) are widespread across cold desert soils and that this process is linked to dry and nutrient-poor environments. We used quantitative PCR (qPCR) to quantify these genes in 122 soil microbiomes across the three poles; spanning the Tibetan Plateau, 10 Antarctic and three high Arctic sites. Both genes were ubiquitous, being present at variable abundances in all 122 soils examined (rbcL1E, 6.25 × 10 3 –1.66 × 10 9 copies/g soil; hhyL, 6.84 × 10 3 –5.07 × 10 8 copies/g soil). For the Antarctic and Arctic sites, random forest and correlation analysis against 26 measured soil physicochemical parameters revealed that rbcL1E and hhyL genes were associated with lower soil moisture, carbon and nitrogen content. While further studies are required to quantify the rates of trace gas carbon fixation and the organisms involved, we highlight the global potential of desert soil microbiomes to be supported by this new minimalistic mode of carbon fixation, particularly throughout dry oligotrophic environments, which encompass more than 35% of the Earth’s surface. Dataset Antarc* Antarctic Arctic Frontiers: Figshare Arctic Antarctic The Antarctic Calvin ENVELOPE(165.100,165.100,-71.283,-71.283) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology carbon fixation atmospheric chemosynthesis trace gases photosynthesis environmental drivers quantitative PCR |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology carbon fixation atmospheric chemosynthesis trace gases photosynthesis environmental drivers quantitative PCR Angelique E. Ray Eden Zhang Aleks Terauds Mukan Ji Weidong Kong Belinda C. Ferrari Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology carbon fixation atmospheric chemosynthesis trace gases photosynthesis environmental drivers quantitative PCR |
description |
Soil microbiomes within oligotrophic cold deserts are extraordinarily diverse. Increasingly, oligotrophic sites with low levels of phototrophic primary producers are reported, leading researchers to question their carbon and energy sources. A novel microbial carbon fixation process termed atmospheric chemosynthesis recently filled this gap as it was shown to be supporting primary production at two Eastern Antarctic deserts. Atmospheric chemosynthesis uses energy liberated from the oxidation of atmospheric hydrogen to drive the Calvin-Benson-Bassham (CBB) cycle through a new chemotrophic form of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), designated IE. Here, we propose that the genetic determinants of this process; RuBisCO type IE (rbcL1E) and high affinity group 1h-[NiFe]-hydrogenase (hhyL) are widespread across cold desert soils and that this process is linked to dry and nutrient-poor environments. We used quantitative PCR (qPCR) to quantify these genes in 122 soil microbiomes across the three poles; spanning the Tibetan Plateau, 10 Antarctic and three high Arctic sites. Both genes were ubiquitous, being present at variable abundances in all 122 soils examined (rbcL1E, 6.25 × 10 3 –1.66 × 10 9 copies/g soil; hhyL, 6.84 × 10 3 –5.07 × 10 8 copies/g soil). For the Antarctic and Arctic sites, random forest and correlation analysis against 26 measured soil physicochemical parameters revealed that rbcL1E and hhyL genes were associated with lower soil moisture, carbon and nitrogen content. While further studies are required to quantify the rates of trace gas carbon fixation and the organisms involved, we highlight the global potential of desert soil microbiomes to be supported by this new minimalistic mode of carbon fixation, particularly throughout dry oligotrophic environments, which encompass more than 35% of the Earth’s surface. |
format |
Dataset |
author |
Angelique E. Ray Eden Zhang Aleks Terauds Mukan Ji Weidong Kong Belinda C. Ferrari |
author_facet |
Angelique E. Ray Eden Zhang Aleks Terauds Mukan Ji Weidong Kong Belinda C. Ferrari |
author_sort |
Angelique E. Ray |
title |
Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX |
title_short |
Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX |
title_full |
Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX |
title_fullStr |
Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX |
title_full_unstemmed |
Data_Sheet_1_Soil Microbiomes With the Genetic Capacity for Atmospheric Chemosynthesis Are Widespread Across the Poles and Are Associated With Moisture, Carbon, and Nitrogen Limitation.DOCX |
title_sort |
data_sheet_1_soil microbiomes with the genetic capacity for atmospheric chemosynthesis are widespread across the poles and are associated with moisture, carbon, and nitrogen limitation.docx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.01936.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Soil_Microbiomes_With_the_Genetic_Capacity_for_Atmospheric_Chemosynthesis_Are_Widespread_Across_the_Poles_and_Are_Associated_With_Moisture_Carbon_and_Nitrogen_Limitation_DOCX/12794903 |
long_lat |
ENVELOPE(165.100,165.100,-71.283,-71.283) |
geographic |
Arctic Antarctic The Antarctic Calvin |
geographic_facet |
Arctic Antarctic The Antarctic Calvin |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
doi:10.3389/fmicb.2020.01936.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Soil_Microbiomes_With_the_Genetic_Capacity_for_Atmospheric_Chemosynthesis_Are_Widespread_Across_the_Poles_and_Are_Associated_With_Moisture_Carbon_and_Nitrogen_Limitation_DOCX/12794903 |
op_doi |
https://doi.org/10.3389/fmicb.2020.01936.s001 |
_version_ |
1766275165566533632 |