Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX
Cryoconite holes are miniature freshwater aquatic ecosystems that harbor a relatively diverse microbial community. This microbial community can withstand the extreme conditions of the supraglacial environment, including fluctuating temperatures, extreme and varying geochemical conditions and limited...
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ftfrontimediafig:oai:figshare.com:article/12745235 2023-05-15T14:04:09+02:00 Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX Ewa A. Poniecka Elizabeth A. Bagshaw Henrik Sass Amelia Segar Gordon Webster Christopher Williamson Alexandre M. Anesio Martyn Tranter 2020-07-31T09:53:55Z https://doi.org/10.3389/fmicb.2020.01783.s001 https://figshare.com/articles/dataset/Table_1_Physiological_Capabilities_of_Cryoconite_Hole_Microorganisms_DOCX/12745235 unknown doi:10.3389/fmicb.2020.01783.s001 https://figshare.com/articles/dataset/Table_1_Physiological_Capabilities_of_Cryoconite_Hole_Microorganisms_DOCX/12745235 Microbiology Microbial Genetics Microbial Ecology Mycology cryoconite microbial physiology cultivation freeze-thaw extreme conditions Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.01783.s001 2020-08-05T22:55:45Z Cryoconite holes are miniature freshwater aquatic ecosystems that harbor a relatively diverse microbial community. This microbial community can withstand the extreme conditions of the supraglacial environment, including fluctuating temperatures, extreme and varying geochemical conditions and limited nutrients. We analyzed the physiological capabilities of microbial isolates from cryoconite holes from Antarctica, Greenland, and Svalbard in selected environmental conditions: extreme pH, salinity, freeze-thaw and limited carbon sources, to identify their physiological limits. The results suggest that heterotrophic microorganisms in cryoconite holes are well adapted to fast-changing environmental conditions, by surviving multiple freeze-thaw cycles, a wide range of salinity and pH conditions and scavenging a variety of organic substrates. Under oxic and anoxic conditions, the communities grew well in temperatures up to 30°C, although in anoxic conditions the community was more successful at colder temperatures (0.2°C). The most abundant cultivable microorganisms were facultative anaerobic bacteria and yeasts. They grew in salinities up to 10% and in pH ranging from 4 to 10.5 (Antarctica), 2.5 to 10 (Svalbard), and 3 to 10 (Greenland). Their growth was sustained on at least 58 single carbon sources and there was no decrease in viability for some isolates after up to 100 consecutive freeze-thaw cycles. The elevated viability of the anaerobic community in the lowest temperatures indicates they might be key players in winter conditions or in early melt seasons, when the oxygen is potentially depleted due to limited flow of meltwater. Consequently, facultative anaerobic heterotrophs are likely important players in the reactivation of the community after the polar night. This detailed physiological investigation shows that despite inhabiting a freshwater environment, cryoconite microorganisms are able to withstand conditions not typically encountered in freshwater environments (namely high salinities or extreme pH), ... Dataset Antarc* Antarctica Greenland polar night Svalbard Frontiers: Figshare Svalbard Greenland |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology cryoconite microbial physiology cultivation freeze-thaw extreme conditions |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology cryoconite microbial physiology cultivation freeze-thaw extreme conditions Ewa A. Poniecka Elizabeth A. Bagshaw Henrik Sass Amelia Segar Gordon Webster Christopher Williamson Alexandre M. Anesio Martyn Tranter Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology cryoconite microbial physiology cultivation freeze-thaw extreme conditions |
description |
Cryoconite holes are miniature freshwater aquatic ecosystems that harbor a relatively diverse microbial community. This microbial community can withstand the extreme conditions of the supraglacial environment, including fluctuating temperatures, extreme and varying geochemical conditions and limited nutrients. We analyzed the physiological capabilities of microbial isolates from cryoconite holes from Antarctica, Greenland, and Svalbard in selected environmental conditions: extreme pH, salinity, freeze-thaw and limited carbon sources, to identify their physiological limits. The results suggest that heterotrophic microorganisms in cryoconite holes are well adapted to fast-changing environmental conditions, by surviving multiple freeze-thaw cycles, a wide range of salinity and pH conditions and scavenging a variety of organic substrates. Under oxic and anoxic conditions, the communities grew well in temperatures up to 30°C, although in anoxic conditions the community was more successful at colder temperatures (0.2°C). The most abundant cultivable microorganisms were facultative anaerobic bacteria and yeasts. They grew in salinities up to 10% and in pH ranging from 4 to 10.5 (Antarctica), 2.5 to 10 (Svalbard), and 3 to 10 (Greenland). Their growth was sustained on at least 58 single carbon sources and there was no decrease in viability for some isolates after up to 100 consecutive freeze-thaw cycles. The elevated viability of the anaerobic community in the lowest temperatures indicates they might be key players in winter conditions or in early melt seasons, when the oxygen is potentially depleted due to limited flow of meltwater. Consequently, facultative anaerobic heterotrophs are likely important players in the reactivation of the community after the polar night. This detailed physiological investigation shows that despite inhabiting a freshwater environment, cryoconite microorganisms are able to withstand conditions not typically encountered in freshwater environments (namely high salinities or extreme pH), ... |
format |
Dataset |
author |
Ewa A. Poniecka Elizabeth A. Bagshaw Henrik Sass Amelia Segar Gordon Webster Christopher Williamson Alexandre M. Anesio Martyn Tranter |
author_facet |
Ewa A. Poniecka Elizabeth A. Bagshaw Henrik Sass Amelia Segar Gordon Webster Christopher Williamson Alexandre M. Anesio Martyn Tranter |
author_sort |
Ewa A. Poniecka |
title |
Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX |
title_short |
Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX |
title_full |
Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX |
title_fullStr |
Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX |
title_full_unstemmed |
Table_1_Physiological Capabilities of Cryoconite Hole Microorganisms.DOCX |
title_sort |
table_1_physiological capabilities of cryoconite hole microorganisms.docx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.01783.s001 https://figshare.com/articles/dataset/Table_1_Physiological_Capabilities_of_Cryoconite_Hole_Microorganisms_DOCX/12745235 |
geographic |
Svalbard Greenland |
geographic_facet |
Svalbard Greenland |
genre |
Antarc* Antarctica Greenland polar night Svalbard |
genre_facet |
Antarc* Antarctica Greenland polar night Svalbard |
op_relation |
doi:10.3389/fmicb.2020.01783.s001 https://figshare.com/articles/dataset/Table_1_Physiological_Capabilities_of_Cryoconite_Hole_Microorganisms_DOCX/12745235 |
op_doi |
https://doi.org/10.3389/fmicb.2020.01783.s001 |
_version_ |
1766275165399810048 |