Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This...
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Online Access: | https://doi.org/10.3389/fimmu.2020.01379.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_CD63_Homolog_Specially_Recruited_to_the_Fungi-Contained_Phagosomes_Is_Involved_in_the_Cellular_Immune_Response_of_Oyster_Crassostrea_gigas_PDF/12686552 |
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ftfrontimediafig:oai:figshare.com:article/12686552 2023-05-15T15:58:22+02:00 Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song 2020-07-22T04:34:46Z https://doi.org/10.3389/fimmu.2020.01379.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_CD63_Homolog_Specially_Recruited_to_the_Fungi-Contained_Phagosomes_Is_Involved_in_the_Cellular_Immune_Response_of_Oyster_Crassostrea_gigas_PDF/12686552 unknown doi:10.3389/fimmu.2020.01379.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_CD63_Homolog_Specially_Recruited_to_the_Fungi-Contained_Phagosomes_Is_Involved_in_the_Cellular_Immune_Response_of_Oyster_Crassostrea_gigas_PDF/12686552 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.01379.s001 2020-07-22T22:53:45Z Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying CgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p < 0.01) upregulated after the injection of Vibrio splendidus. CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica-containing phagosomes after the stimulation of Y. lipolytica. The recombinant CgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited (p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that CgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas. Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas |
description |
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying CgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p < 0.01) upregulated after the injection of Vibrio splendidus. CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica-containing phagosomes after the stimulation of Y. lipolytica. The recombinant CgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited (p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that CgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas. |
format |
Dataset |
author |
Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song |
author_facet |
Conghui Liu Chuanyan Yang Mengqiang Wang Shuai Jiang Qilin Yi Weilin Wang Lingling Wang Linsheng Song |
author_sort |
Conghui Liu |
title |
Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF |
title_short |
Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF |
title_full |
Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF |
title_fullStr |
Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF |
title_full_unstemmed |
Data_Sheet_1_A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas.PDF |
title_sort |
data_sheet_1_a cd63 homolog specially recruited to the fungi-contained phagosomes is involved in the cellular immune response of oyster crassostrea gigas.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.01379.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_CD63_Homolog_Specially_Recruited_to_the_Fungi-Contained_Phagosomes_Is_Involved_in_the_Cellular_Immune_Response_of_Oyster_Crassostrea_gigas_PDF/12686552 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2020.01379.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_CD63_Homolog_Specially_Recruited_to_the_Fungi-Contained_Phagosomes_Is_Involved_in_the_Cellular_Immune_Response_of_Oyster_Crassostrea_gigas_PDF/12686552 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.01379.s001 |
_version_ |
1766394105185697792 |