Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf
Microbes associated with sinking marine particles play key roles in carbon sequestration in the ocean. The sampling of particle-attached microorganisms is often done with sediment traps or by filtration of water collected with oceanographic bottles, both involving a certain time lapse between collec...
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Online Access: | https://doi.org/10.3389/fmicb.2020.01645.s001 https://figshare.com/articles/figure/Image_1_Sampling_Device-Dependence_of_Prokaryotic_Community_Structure_on_Marine_Particles_Higher_Diversity_Recovered_by_in_situ_Pumps_Than_by_Oceanographic_Bottles_pdf/12654791 |
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ftfrontimediafig:oai:figshare.com:article/12654791 2023-05-15T14:04:47+02:00 Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf Viena Puigcorbé Clara Ruiz-González Pere Masqué Josep M. Gasol 2020-07-15T04:12:22Z https://doi.org/10.3389/fmicb.2020.01645.s001 https://figshare.com/articles/figure/Image_1_Sampling_Device-Dependence_of_Prokaryotic_Community_Structure_on_Marine_Particles_Higher_Diversity_Recovered_by_in_situ_Pumps_Than_by_Oceanographic_Bottles_pdf/12654791 unknown doi:10.3389/fmicb.2020.01645.s001 https://figshare.com/articles/figure/Image_1_Sampling_Device-Dependence_of_Prokaryotic_Community_Structure_on_Marine_Particles_Higher_Diversity_Recovered_by_in_situ_Pumps_Than_by_Oceanographic_Bottles_pdf/12654791 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology prokaryotic communities marine particles size-fractionation in situ pumps oceanographic bottles polynyas Image Figure 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.01645.s001 2020-07-15T22:53:49Z Microbes associated with sinking marine particles play key roles in carbon sequestration in the ocean. The sampling of particle-attached microorganisms is often done with sediment traps or by filtration of water collected with oceanographic bottles, both involving a certain time lapse between collection and processing of samples that may result in changes in particle-attached microbial communities. Conversely, in situ water filtration through submersible pumps allows a faster storage of sampled particles, but it has rarely been used to study the associated microbial communities and has never been compared to other particle-sampling methods in terms of the recovery of particle microbial diversity. Here we compared the prokaryotic communities attached to small (1–53 μm) and large (>53 μm) particles collected from the mesopelagic zone (100–300 m) of two Antarctic polynyas using in situ pumps (ISP) and oceanographic bottles (BTL). Each sampling method retrieved largely different particle-attached communities, suggesting that they capture different kinds of particles. These device-driven differences were greater for large particles than for small particles. Overall, the ISP recovered 1.5- to 3-fold more particle-attached bacterial taxa than the BTL, and different taxonomic groups were preferentially recovered by each method. In particular, typical particle-attached groups such as Planctomycetes and Deltaproteobacteria recovered with ISP were nearly absent from BTL samples. Our results suggest that the method used to sample marine particles has a strong influence in our view of their associated microbial communities. Still Image Antarc* Antarctic Frontiers: Figshare Antarctic |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology prokaryotic communities marine particles size-fractionation in situ pumps oceanographic bottles polynyas |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology prokaryotic communities marine particles size-fractionation in situ pumps oceanographic bottles polynyas Viena Puigcorbé Clara Ruiz-González Pere Masqué Josep M. Gasol Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology prokaryotic communities marine particles size-fractionation in situ pumps oceanographic bottles polynyas |
description |
Microbes associated with sinking marine particles play key roles in carbon sequestration in the ocean. The sampling of particle-attached microorganisms is often done with sediment traps or by filtration of water collected with oceanographic bottles, both involving a certain time lapse between collection and processing of samples that may result in changes in particle-attached microbial communities. Conversely, in situ water filtration through submersible pumps allows a faster storage of sampled particles, but it has rarely been used to study the associated microbial communities and has never been compared to other particle-sampling methods in terms of the recovery of particle microbial diversity. Here we compared the prokaryotic communities attached to small (1–53 μm) and large (>53 μm) particles collected from the mesopelagic zone (100–300 m) of two Antarctic polynyas using in situ pumps (ISP) and oceanographic bottles (BTL). Each sampling method retrieved largely different particle-attached communities, suggesting that they capture different kinds of particles. These device-driven differences were greater for large particles than for small particles. Overall, the ISP recovered 1.5- to 3-fold more particle-attached bacterial taxa than the BTL, and different taxonomic groups were preferentially recovered by each method. In particular, typical particle-attached groups such as Planctomycetes and Deltaproteobacteria recovered with ISP were nearly absent from BTL samples. Our results suggest that the method used to sample marine particles has a strong influence in our view of their associated microbial communities. |
format |
Still Image |
author |
Viena Puigcorbé Clara Ruiz-González Pere Masqué Josep M. Gasol |
author_facet |
Viena Puigcorbé Clara Ruiz-González Pere Masqué Josep M. Gasol |
author_sort |
Viena Puigcorbé |
title |
Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf |
title_short |
Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf |
title_full |
Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf |
title_fullStr |
Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf |
title_full_unstemmed |
Image_1_Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles: Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles.pdf |
title_sort |
image_1_sampling device-dependence of prokaryotic community structure on marine particles: higher diversity recovered by in situ pumps than by oceanographic bottles.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.01645.s001 https://figshare.com/articles/figure/Image_1_Sampling_Device-Dependence_of_Prokaryotic_Community_Structure_on_Marine_Particles_Higher_Diversity_Recovered_by_in_situ_Pumps_Than_by_Oceanographic_Bottles_pdf/12654791 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.3389/fmicb.2020.01645.s001 https://figshare.com/articles/figure/Image_1_Sampling_Device-Dependence_of_Prokaryotic_Community_Structure_on_Marine_Particles_Higher_Diversity_Recovered_by_in_situ_Pumps_Than_by_Oceanographic_Bottles_pdf/12654791 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.01645.s001 |
_version_ |
1766276076133154816 |