Image_1_Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent.pdf

• Main Authors: Yangxin Wang, Ningning Zhang, Deming Tan, Zhenhui Qi, Changzhu Wu
• Format: Still Image
• Language: unknown
• Published: 2020
• Subjects: Biotechnology; Biological Engineering; Genetic Engineering; Biomarkers; Biomaterials; Biomechanical Engineering; Biomedical Engineering not elsewhere classified; Synthetic Biology; Agricultural Marine Biotechnology; Bioremediation; Bioprocessing; Bioproduction and Bioproducts; Industrial Biotechnology Diagnostics (incl. Biosensors); Industrial Microbiology (incl. Biofeedstocks); Industrial Molecular Engineering of Nucleic Acids and Proteins; Industrial Biotechnology not elsewhere classified; Medical Biotechnology Diagnostics (incl. Biosensors); Medical Molecular Engineering of Nucleic Acids and Proteins; Regenerative Medicine (incl. Stem Cells and Tissue Engineering); Medical Biotechnology not elsewhere classified; metal–organic frameworks; enzymatic reactions; size-selective catalysis; heterogeneous catalysis; biocatalysis; Antarc*; Antarctica
• Online Access: https://doi.org/10.3389/fbioe.2020.00714.s001; https://figshare.com/articles/figure/Image_1_Facile_Synthesis_of_Enzyme-Embedded_Metal_Organic_Frameworks_for_Size-Selective_Biocatalysis_in_Organic_Solvent_pdf/12616973

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO 3 ) 2 ⋅6H 2 O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.