Image_1_Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent.pdf

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahy...

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Bibliographic Details
Main Authors: Yangxin Wang, Ningning Zhang, Deming Tan, Zhenhui Qi, Changzhu Wu
Format: Still Image
Language:unknown
Published: 2020
Subjects:
Biotechnology
Biological Engineering
Genetic Engineering
Biomarkers
Biomaterials
Biomechanical Engineering
Biomedical Engineering not elsewhere classified
Synthetic Biology
Agricultural Marine Biotechnology
Bioremediation
Bioprocessing
Bioproduction and Bioproducts
Industrial Biotechnology Diagnostics (incl. Biosensors)
Industrial Microbiology (incl. Biofeedstocks)
Industrial Molecular Engineering of Nucleic Acids and Proteins
Industrial Biotechnology not elsewhere classified
Medical Biotechnology Diagnostics (incl. Biosensors)
Medical Molecular Engineering of Nucleic Acids and Proteins
Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
Medical Biotechnology not elsewhere classified
metal–organic frameworks
enzymatic reactions
size-selective catalysis
heterogeneous catalysis
biocatalysis
Antarc*
Antarctica
Online Access:https://doi.org/10.3389/fbioe.2020.00714.s001
https://figshare.com/articles/figure/Image_1_Facile_Synthesis_of_Enzyme-Embedded_Metal_Organic_Frameworks_for_Size-Selective_Biocatalysis_in_Organic_Solvent_pdf/12616973
Description
Summary:In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO 3 ) 2 ⋅6H 2 O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

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