Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf
Climate change alters environmental conditions that are expected to have a profound effect on the biodiversity, community composition, and metabolic processes of microeukaryotic plankton in Arctic and Subarctic coastal waters. The molecular biodiversity [large subunit (LSU) rRNA gene] of three plank...
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ftfrontimediafig:oai:figshare.com:article/12572090 2023-05-15T14:55:52+02:00 Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf Stephanie Elferink Sylke Wohlrab Stefan Neuhaus Allan Cembella Lars Harms Uwe John 2020-06-26T13:29:30Z https://doi.org/10.3389/fmars.2020.00439.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comparative_Metabarcoding_and_Metatranscriptomic_Analysis_of_Microeukaryotes_Within_Coastal_Surface_Waters_of_West_Greenland_and_Northwest_Iceland_pdf/12572090 unknown doi:10.3389/fmars.2020.00439.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comparative_Metabarcoding_and_Metatranscriptomic_Analysis_of_Microeukaryotes_Within_Coastal_Surface_Waters_of_West_Greenland_and_Northwest_Iceland_pdf/12572090 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering arctic microplankton DNA-barcoding metabolic processes microeukaryotic community marine pelagic community picoplankton Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmars.2020.00439.s001 2020-07-01T22:55:04Z Climate change alters environmental conditions that are expected to have a profound effect on the biodiversity, community composition, and metabolic processes of microeukaryotic plankton in Arctic and Subarctic coastal waters. The molecular biodiversity [large subunit (LSU) rRNA gene] of three plankton size-fractions (micro-, nano-, and picoplankton) from coastal waters of ice-influenced west Greenland was compared with fractions from ice-free northwest Iceland within their summer environmental context. Putative metabolic functions were determined by differentially expressed mRNA (metatranscriptomics) of the microplankton. Temperature and salinity variations were more closely correlated than inorganic macronutrients with metabolic functions and community composition. Temperature explained much of the community variance, approximately 20% among micro- and nanoplankton, whereas other environmental factors accounted for rather low fractional variance (<7%). Species of smaller cell-size were more evenly distributed (Pielou’s evenness index J) across regions, with a higher diversity and total abundance, and thereby indicating high plasticity. The metatranscriptomic profiles in these respective microeukaryotic communities revealed that diatoms were more plastic in their gene expression than dinoflagellates, but dinoflagellates had a more diverse, albeit homogeneously expressed, gene pool. This could be interpreted as expression of alternative lifestyle strategies, whereby the functionally more conservative diatoms fill their niches primarily through variable resource use, whereas dinoflagellates apparently differentiate their niches through more diverse lifestyles. Patterns of microeukaryotic diversity are thus primarily associated with differences in metabolic function and activity of diatom- versus dinoflagellate-dominated communities in Arctic and Subarctic waters during summer. Dataset Arctic Climate change Greenland Iceland Subarctic Frontiers: Figshare Arctic Greenland |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering arctic microplankton DNA-barcoding metabolic processes microeukaryotic community marine pelagic community picoplankton |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering arctic microplankton DNA-barcoding metabolic processes microeukaryotic community marine pelagic community picoplankton Stephanie Elferink Sylke Wohlrab Stefan Neuhaus Allan Cembella Lars Harms Uwe John Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering arctic microplankton DNA-barcoding metabolic processes microeukaryotic community marine pelagic community picoplankton |
description |
Climate change alters environmental conditions that are expected to have a profound effect on the biodiversity, community composition, and metabolic processes of microeukaryotic plankton in Arctic and Subarctic coastal waters. The molecular biodiversity [large subunit (LSU) rRNA gene] of three plankton size-fractions (micro-, nano-, and picoplankton) from coastal waters of ice-influenced west Greenland was compared with fractions from ice-free northwest Iceland within their summer environmental context. Putative metabolic functions were determined by differentially expressed mRNA (metatranscriptomics) of the microplankton. Temperature and salinity variations were more closely correlated than inorganic macronutrients with metabolic functions and community composition. Temperature explained much of the community variance, approximately 20% among micro- and nanoplankton, whereas other environmental factors accounted for rather low fractional variance (<7%). Species of smaller cell-size were more evenly distributed (Pielou’s evenness index J) across regions, with a higher diversity and total abundance, and thereby indicating high plasticity. The metatranscriptomic profiles in these respective microeukaryotic communities revealed that diatoms were more plastic in their gene expression than dinoflagellates, but dinoflagellates had a more diverse, albeit homogeneously expressed, gene pool. This could be interpreted as expression of alternative lifestyle strategies, whereby the functionally more conservative diatoms fill their niches primarily through variable resource use, whereas dinoflagellates apparently differentiate their niches through more diverse lifestyles. Patterns of microeukaryotic diversity are thus primarily associated with differences in metabolic function and activity of diatom- versus dinoflagellate-dominated communities in Arctic and Subarctic waters during summer. |
format |
Dataset |
author |
Stephanie Elferink Sylke Wohlrab Stefan Neuhaus Allan Cembella Lars Harms Uwe John |
author_facet |
Stephanie Elferink Sylke Wohlrab Stefan Neuhaus Allan Cembella Lars Harms Uwe John |
author_sort |
Stephanie Elferink |
title |
Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf |
title_short |
Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf |
title_full |
Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf |
title_fullStr |
Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf |
title_full_unstemmed |
Data_Sheet_1_Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland.pdf |
title_sort |
data_sheet_1_comparative metabarcoding and metatranscriptomic analysis of microeukaryotes within coastal surface waters of west greenland and northwest iceland.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmars.2020.00439.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comparative_Metabarcoding_and_Metatranscriptomic_Analysis_of_Microeukaryotes_Within_Coastal_Surface_Waters_of_West_Greenland_and_Northwest_Iceland_pdf/12572090 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Climate change Greenland Iceland Subarctic |
genre_facet |
Arctic Climate change Greenland Iceland Subarctic |
op_relation |
doi:10.3389/fmars.2020.00439.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comparative_Metabarcoding_and_Metatranscriptomic_Analysis_of_Microeukaryotes_Within_Coastal_Surface_Waters_of_West_Greenland_and_Northwest_Iceland_pdf/12572090 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2020.00439.s001 |
_version_ |
1766327877498830848 |