Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF
Due to ocean acidification and global warming, surface seawater of the western Baltic Sea is expected to reach an average of ∼1100 μatm pCO 2 and an increase of ∼5°C by the year 2100. In four consecutive experiments (spanning 10–11 weeks each) in all seasons within 1 year, the abiotic factors temper...
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ftfrontimediafig:oai:figshare.com:article/12571247 2023-05-15T17:51:51+02:00 Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF Birte Mensch Sven C. Neulinger Sven Künzel Martin Wahl Ruth A. Schmitz 2020-06-26T11:50:20Z https://doi.org/10.3389/fmicb.2020.01471.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Warming_but_Not_Acidification_Restructures_Epibacterial_Communities_of_the_Baltic_Macroalga_Fucus_vesiculosus_With_Seasonal_Variability_PDF/12571247 unknown doi:10.3389/fmicb.2020.01471.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Warming_but_Not_Acidification_Restructures_Epibacterial_Communities_of_the_Baltic_Macroalga_Fucus_vesiculosus_With_Seasonal_Variability_PDF/12571247 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology bacterial community structure 16S rDNA amplicon sequencing temperature pCO2 benthocosm macroalgal holobiont Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.01471.s001 2020-07-01T22:55:11Z Due to ocean acidification and global warming, surface seawater of the western Baltic Sea is expected to reach an average of ∼1100 μatm pCO 2 and an increase of ∼5°C by the year 2100. In four consecutive experiments (spanning 10–11 weeks each) in all seasons within 1 year, the abiotic factors temperature (+5°C above in situ) and pCO 2 (adjusted to ∼1100 μatm) were tested for their single and combined effects on epibacterial communities of the brown macroalga Fucus vesiculosus and on bacteria present in the surrounding seawater. The experiments were set up in three biological replicates using the Kiel Outdoor Benthocosm facility (Kiel, Germany). Phylogenetic analyses of the respective microbiota were performed by bacterial 16S (V1-V2) rDNA Illumina MiSeq amplicon sequencing after 0, 4, 8, and 10/11 weeks per season. The results demonstrate (I) that the bacterial community composition varied in time and (II) that relationships between operational taxonomic units (OTUs) within an OTU association network were mainly governed by the habitat. (III) Neither single pCO 2 nor pCO 2 :Temperature interaction effects were statistically significant. However, significant impact of ocean warming was detected varying among seasons. (IV) An indicator OTU (iOTU) analysis identified several iOTUs that were strongly influenced by temperature in spring, summer, and winter. In the warming treatments of these three seasons, we observed decreasing numbers of bacteria that are commonly associated with a healthy marine microbial community and—particularly during spring and summer—an increase in potentially pathogenic and bacteria related to intensified microfouling. This might lead to severe consequences for the F. vesiculosus holobiont finally affecting the marine ecosystem. Dataset Ocean acidification Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology bacterial community structure 16S rDNA amplicon sequencing temperature pCO2 benthocosm macroalgal holobiont |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology bacterial community structure 16S rDNA amplicon sequencing temperature pCO2 benthocosm macroalgal holobiont Birte Mensch Sven C. Neulinger Sven Künzel Martin Wahl Ruth A. Schmitz Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology bacterial community structure 16S rDNA amplicon sequencing temperature pCO2 benthocosm macroalgal holobiont |
description |
Due to ocean acidification and global warming, surface seawater of the western Baltic Sea is expected to reach an average of ∼1100 μatm pCO 2 and an increase of ∼5°C by the year 2100. In four consecutive experiments (spanning 10–11 weeks each) in all seasons within 1 year, the abiotic factors temperature (+5°C above in situ) and pCO 2 (adjusted to ∼1100 μatm) were tested for their single and combined effects on epibacterial communities of the brown macroalga Fucus vesiculosus and on bacteria present in the surrounding seawater. The experiments were set up in three biological replicates using the Kiel Outdoor Benthocosm facility (Kiel, Germany). Phylogenetic analyses of the respective microbiota were performed by bacterial 16S (V1-V2) rDNA Illumina MiSeq amplicon sequencing after 0, 4, 8, and 10/11 weeks per season. The results demonstrate (I) that the bacterial community composition varied in time and (II) that relationships between operational taxonomic units (OTUs) within an OTU association network were mainly governed by the habitat. (III) Neither single pCO 2 nor pCO 2 :Temperature interaction effects were statistically significant. However, significant impact of ocean warming was detected varying among seasons. (IV) An indicator OTU (iOTU) analysis identified several iOTUs that were strongly influenced by temperature in spring, summer, and winter. In the warming treatments of these three seasons, we observed decreasing numbers of bacteria that are commonly associated with a healthy marine microbial community and—particularly during spring and summer—an increase in potentially pathogenic and bacteria related to intensified microfouling. This might lead to severe consequences for the F. vesiculosus holobiont finally affecting the marine ecosystem. |
format |
Dataset |
author |
Birte Mensch Sven C. Neulinger Sven Künzel Martin Wahl Ruth A. Schmitz |
author_facet |
Birte Mensch Sven C. Neulinger Sven Künzel Martin Wahl Ruth A. Schmitz |
author_sort |
Birte Mensch |
title |
Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF |
title_short |
Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF |
title_full |
Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF |
title_fullStr |
Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF |
title_full_unstemmed |
Data_Sheet_1_Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.PDF |
title_sort |
data_sheet_1_warming, but not acidification, restructures epibacterial communities of the baltic macroalga fucus vesiculosus with seasonal variability.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.01471.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Warming_but_Not_Acidification_Restructures_Epibacterial_Communities_of_the_Baltic_Macroalga_Fucus_vesiculosus_With_Seasonal_Variability_PDF/12571247 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.3389/fmicb.2020.01471.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Warming_but_Not_Acidification_Restructures_Epibacterial_Communities_of_the_Baltic_Macroalga_Fucus_vesiculosus_With_Seasonal_Variability_PDF/12571247 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.01471.s001 |
_version_ |
1766159129311707136 |