Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG
Salmon gill poxvirus (SGPV) can cause serious gill disease in Atlantic salmon (Salmo salar L.) and represents a significant problem to aquaculture industries in Northern Europe. Here, a single-tube multi-locus variable-number tandem-repeat (VNTR) analysis (MLVA) genotyping assay, targeting eight VNT...
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2020
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Online Access: | https://doi.org/10.3389/fmicb.2020.01071.s002 https://figshare.com/articles/Image_1_Genotyping_of_Salmon_Gill_Poxvirus_Reveals_One_Main_Predominant_Lineage_in_Europe_Featuring_Fjord-_and_Fish_Farm-Specific_Sub-Lineages_JPEG/12386516 |
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ftfrontimediafig:oai:figshare.com:article/12386516 2023-05-15T15:31:29+02:00 Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG Snorre Gulla Torstein Tengs Saima Nasrin Mohammad Mona Gjessing Åse Helen Garseth Karoline Sveinsson Torfinn Moldal Petra E. Petersen Brit Tørud Ole Bendik Dale Maria K. Dahle 2020-05-29T04:03:32Z https://doi.org/10.3389/fmicb.2020.01071.s002 https://figshare.com/articles/Image_1_Genotyping_of_Salmon_Gill_Poxvirus_Reveals_One_Main_Predominant_Lineage_in_Europe_Featuring_Fjord-_and_Fish_Farm-Specific_Sub-Lineages_JPEG/12386516 unknown doi:10.3389/fmicb.2020.01071.s002 https://figshare.com/articles/Image_1_Genotyping_of_Salmon_Gill_Poxvirus_Reveals_One_Main_Predominant_Lineage_in_Europe_Featuring_Fjord-_and_Fish_Farm-Specific_Sub-Lineages_JPEG/12386516 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology salmon gill poxvirus (SGPV) MLVA VNTR microsatellite genotyping fish disease Atlantic salmon (Salmo salar) aquaculture Image Figure 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.01071.s002 2020-06-03T22:55:04Z Salmon gill poxvirus (SGPV) can cause serious gill disease in Atlantic salmon (Salmo salar L.) and represents a significant problem to aquaculture industries in Northern Europe. Here, a single-tube multi-locus variable-number tandem-repeat (VNTR) analysis (MLVA) genotyping assay, targeting eight VNTR loci, was developed for studying the epizootiology of SGPV. Through MLVA typing of SGPV positive samples from 180 farmed and wild Atlantic salmon in Northern Europe, the first molecular population study of this virus was undertaken. Comparison of resulting MLVA profiles by cluster analysis revealed considerable micro-diversity, while only a limited degree of specific clustering by country of origin could be observed, and no clustering relating to the severity of disease outbreaks. Phylogenetic analysis, based on genomic data from six SGPV specimens (three Norwegian, one Scottish, one Faroese and one Canadian), complemented and corroborated MLVA by pointing to a marked transatlantic divide in the species, with one main, relatively conserved, SGPV lineage as predominant in Europe. Within certain fjord systems and individual freshwater salmon smolt farms in Norway, however, discrete MLVA clustering patterns that prevailed over time were observed, likely reflecting local predominance of specific SGPV sub-lineages. MLVA typing was also used to refute two suspected instances of vertical SGPV transmission from salmon broodstock to offspring, and to confirm a failed disinfection attempt in one farm. These novel insights into the previously undocumented population structure of SGPV provide important clues, e.g., regarding the mechanisms underlying spread and recurrence of the virus amongst wild and farmed salmon populations, but so far no indications of more or less virulent SGPV sub-lineages have been found. The MLVA scheme represents a highly sensitive genotyping tool particularly well suited for illuminating SGPV infection routes, and adds to the relatively low number of MLVA protocols that have so far been published for ... Still Image Atlantic salmon Salmo salar Frontiers: Figshare Norway |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology salmon gill poxvirus (SGPV) MLVA VNTR microsatellite genotyping fish disease Atlantic salmon (Salmo salar) aquaculture |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology salmon gill poxvirus (SGPV) MLVA VNTR microsatellite genotyping fish disease Atlantic salmon (Salmo salar) aquaculture Snorre Gulla Torstein Tengs Saima Nasrin Mohammad Mona Gjessing Åse Helen Garseth Karoline Sveinsson Torfinn Moldal Petra E. Petersen Brit Tørud Ole Bendik Dale Maria K. Dahle Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology salmon gill poxvirus (SGPV) MLVA VNTR microsatellite genotyping fish disease Atlantic salmon (Salmo salar) aquaculture |
description |
Salmon gill poxvirus (SGPV) can cause serious gill disease in Atlantic salmon (Salmo salar L.) and represents a significant problem to aquaculture industries in Northern Europe. Here, a single-tube multi-locus variable-number tandem-repeat (VNTR) analysis (MLVA) genotyping assay, targeting eight VNTR loci, was developed for studying the epizootiology of SGPV. Through MLVA typing of SGPV positive samples from 180 farmed and wild Atlantic salmon in Northern Europe, the first molecular population study of this virus was undertaken. Comparison of resulting MLVA profiles by cluster analysis revealed considerable micro-diversity, while only a limited degree of specific clustering by country of origin could be observed, and no clustering relating to the severity of disease outbreaks. Phylogenetic analysis, based on genomic data from six SGPV specimens (three Norwegian, one Scottish, one Faroese and one Canadian), complemented and corroborated MLVA by pointing to a marked transatlantic divide in the species, with one main, relatively conserved, SGPV lineage as predominant in Europe. Within certain fjord systems and individual freshwater salmon smolt farms in Norway, however, discrete MLVA clustering patterns that prevailed over time were observed, likely reflecting local predominance of specific SGPV sub-lineages. MLVA typing was also used to refute two suspected instances of vertical SGPV transmission from salmon broodstock to offspring, and to confirm a failed disinfection attempt in one farm. These novel insights into the previously undocumented population structure of SGPV provide important clues, e.g., regarding the mechanisms underlying spread and recurrence of the virus amongst wild and farmed salmon populations, but so far no indications of more or less virulent SGPV sub-lineages have been found. The MLVA scheme represents a highly sensitive genotyping tool particularly well suited for illuminating SGPV infection routes, and adds to the relatively low number of MLVA protocols that have so far been published for ... |
format |
Still Image |
author |
Snorre Gulla Torstein Tengs Saima Nasrin Mohammad Mona Gjessing Åse Helen Garseth Karoline Sveinsson Torfinn Moldal Petra E. Petersen Brit Tørud Ole Bendik Dale Maria K. Dahle |
author_facet |
Snorre Gulla Torstein Tengs Saima Nasrin Mohammad Mona Gjessing Åse Helen Garseth Karoline Sveinsson Torfinn Moldal Petra E. Petersen Brit Tørud Ole Bendik Dale Maria K. Dahle |
author_sort |
Snorre Gulla |
title |
Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG |
title_short |
Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG |
title_full |
Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG |
title_fullStr |
Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG |
title_full_unstemmed |
Image_1_Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.JPEG |
title_sort |
image_1_genotyping of salmon gill poxvirus reveals one main predominant lineage in europe, featuring fjord- and fish farm-specific sub-lineages.jpeg |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.01071.s002 https://figshare.com/articles/Image_1_Genotyping_of_Salmon_Gill_Poxvirus_Reveals_One_Main_Predominant_Lineage_in_Europe_Featuring_Fjord-_and_Fish_Farm-Specific_Sub-Lineages_JPEG/12386516 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fmicb.2020.01071.s002 https://figshare.com/articles/Image_1_Genotyping_of_Salmon_Gill_Poxvirus_Reveals_One_Main_Predominant_Lineage_in_Europe_Featuring_Fjord-_and_Fish_Farm-Specific_Sub-Lineages_JPEG/12386516 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.01071.s002 |
_version_ |
1766362003268435968 |