Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF
Hemocytes play unequivocally central roles in host immune defense of bivalve mollusks, though the exact mechanisms underlying their functional differentiation are only partially understood. To this end, granulocytes and hyalinocytes were sorted via flow cytometry from hemocytes of the Pacific oyster...
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ftfrontimediafig:oai:figshare.com:article/12378167 2023-05-15T15:59:00+02:00 Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF Fan Mao Nai-Kei Wong Yue Lin Xiangyu Zhang Kunna Liu Minwei Huang Duo Xu Zhiming Xiang Jun Li Yang Zhang Ziniu Yu 2020-05-27T15:12:15Z https://doi.org/10.3389/fimmu.2020.00911.s003 https://figshare.com/articles/Image_3_Transcriptomic_Evidence_Reveals_the_Molecular_Basis_for_Functional_Differentiation_of_Hemocytes_in_a_Marine_Invertebrate_Crassostrea_gigas_TIF/12378167 unknown doi:10.3389/fimmu.2020.00911.s003 https://figshare.com/articles/Image_3_Transcriptomic_Evidence_Reveals_the_Molecular_Basis_for_Functional_Differentiation_of_Hemocytes_in_a_Marine_Invertebrate_Crassostrea_gigas_TIF/12378167 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology oyster functional differentiation granulocytes Cdc42 Fos Image Figure 2020 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.00911.s003 2020-05-27T22:53:15Z Hemocytes play unequivocally central roles in host immune defense of bivalve mollusks, though the exact mechanisms underlying their functional differentiation are only partially understood. To this end, granulocytes and hyalinocytes were sorted via flow cytometry from hemocytes of the Pacific oyster Crassostrea gigas, and consequently quantitative transcriptomic analysis revealed a striking array of differentially expressed genes (DEGs), which were globally upregulated in granulocytes, dedicating to functional differentiation among oyster hemocytes. Our network of DEGs illustrated actively engaged signaling pathways, with Cdc42/Cdc42l being a core regulator of pathway network, which was validated by a dramatically reduced capacity for hemocyte phagocytosis in the presence of Cdc42 inhibitors. Additionally, a number of transcription factors were identified among DEGs, including ELK, HELT, and Fos, which were predominantly expressed in granulocytes. The AP-1 transcription factor Fos was confirmed to facilitate functional differentiation of hemocytes in an assay on binding to target genes by the AP-1 binding site, consistent with downstream phagocytosis and ROS production. Importantly, Cdc42/Cdc42l were also regulated by the expression of Fos, providing a possible regulatory mechanism-guided hemocyte functional differentiation. Findings in this study have bridged a knowledge gap on the mechanistic underpinnings of functional differentiation of hemocytes in a marine invertebrate C. gigas, which promise to facilitate research on the evolution of immune defense and functional differentiation of phagocyte in higher-order and more recent phyla. Still Image Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology oyster functional differentiation granulocytes Cdc42 Fos |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology oyster functional differentiation granulocytes Cdc42 Fos Fan Mao Nai-Kei Wong Yue Lin Xiangyu Zhang Kunna Liu Minwei Huang Duo Xu Zhiming Xiang Jun Li Yang Zhang Ziniu Yu Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology oyster functional differentiation granulocytes Cdc42 Fos |
description |
Hemocytes play unequivocally central roles in host immune defense of bivalve mollusks, though the exact mechanisms underlying their functional differentiation are only partially understood. To this end, granulocytes and hyalinocytes were sorted via flow cytometry from hemocytes of the Pacific oyster Crassostrea gigas, and consequently quantitative transcriptomic analysis revealed a striking array of differentially expressed genes (DEGs), which were globally upregulated in granulocytes, dedicating to functional differentiation among oyster hemocytes. Our network of DEGs illustrated actively engaged signaling pathways, with Cdc42/Cdc42l being a core regulator of pathway network, which was validated by a dramatically reduced capacity for hemocyte phagocytosis in the presence of Cdc42 inhibitors. Additionally, a number of transcription factors were identified among DEGs, including ELK, HELT, and Fos, which were predominantly expressed in granulocytes. The AP-1 transcription factor Fos was confirmed to facilitate functional differentiation of hemocytes in an assay on binding to target genes by the AP-1 binding site, consistent with downstream phagocytosis and ROS production. Importantly, Cdc42/Cdc42l were also regulated by the expression of Fos, providing a possible regulatory mechanism-guided hemocyte functional differentiation. Findings in this study have bridged a knowledge gap on the mechanistic underpinnings of functional differentiation of hemocytes in a marine invertebrate C. gigas, which promise to facilitate research on the evolution of immune defense and functional differentiation of phagocyte in higher-order and more recent phyla. |
format |
Still Image |
author |
Fan Mao Nai-Kei Wong Yue Lin Xiangyu Zhang Kunna Liu Minwei Huang Duo Xu Zhiming Xiang Jun Li Yang Zhang Ziniu Yu |
author_facet |
Fan Mao Nai-Kei Wong Yue Lin Xiangyu Zhang Kunna Liu Minwei Huang Duo Xu Zhiming Xiang Jun Li Yang Zhang Ziniu Yu |
author_sort |
Fan Mao |
title |
Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF |
title_short |
Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF |
title_full |
Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF |
title_fullStr |
Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF |
title_full_unstemmed |
Image_3_Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas.TIF |
title_sort |
image_3_transcriptomic evidence reveals the molecular basis for functional differentiation of hemocytes in a marine invertebrate, crassostrea gigas.tif |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.00911.s003 https://figshare.com/articles/Image_3_Transcriptomic_Evidence_Reveals_the_Molecular_Basis_for_Functional_Differentiation_of_Hemocytes_in_a_Marine_Invertebrate_Crassostrea_gigas_TIF/12378167 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2020.00911.s003 https://figshare.com/articles/Image_3_Transcriptomic_Evidence_Reveals_the_Molecular_Basis_for_Functional_Differentiation_of_Hemocytes_in_a_Marine_Invertebrate_Crassostrea_gigas_TIF/12378167 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.00911.s003 |
_version_ |
1766394779487174656 |