Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly unde...
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ftfrontimediafig:oai:figshare.com:article/12292277 2023-05-15T14:58:00+02:00 Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen 2020-05-13T04:28:17Z https://doi.org/10.3389/fpls.2020.00561.s001 https://figshare.com/articles/Data_Sheet_1_Tissue-Specific_Dynamics_in_the_Endophytic_Bacterial_Communities_in_Arctic_Pioneer_Plant_Oxyria_digyna_xlsx/12292277 unknown doi:10.3389/fpls.2020.00561.s001 https://figshare.com/articles/Data_Sheet_1_Tissue-Specific_Dynamics_in_the_Endophytic_Bacterial_Communities_in_Arctic_Pioneer_Plant_Oxyria_digyna_xlsx/12292277 CC BY 4.0 CC-BY Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fpls.2020.00561.s001 2020-05-13T22:53:10Z The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this ... Dataset Arctic Oxyria digyna Frontiers: Figshare Arctic |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria |
spellingShingle |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx |
topic_facet |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria |
description |
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this ... |
format |
Dataset |
author |
Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen |
author_facet |
Cindy Given Elina Häikiö Manoj Kumar Riitta Nissinen |
author_sort |
Cindy Given |
title |
Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx |
title_short |
Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx |
title_full |
Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx |
title_fullStr |
Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx |
title_full_unstemmed |
Data_Sheet_1_Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna.xlsx |
title_sort |
data_sheet_1_tissue-specific dynamics in the endophytic bacterial communities in arctic pioneer plant oxyria digyna.xlsx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fpls.2020.00561.s001 https://figshare.com/articles/Data_Sheet_1_Tissue-Specific_Dynamics_in_the_Endophytic_Bacterial_Communities_in_Arctic_Pioneer_Plant_Oxyria_digyna_xlsx/12292277 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Oxyria digyna |
genre_facet |
Arctic Oxyria digyna |
op_relation |
doi:10.3389/fpls.2020.00561.s001 https://figshare.com/articles/Data_Sheet_1_Tissue-Specific_Dynamics_in_the_Endophytic_Bacterial_Communities_in_Arctic_Pioneer_Plant_Oxyria_digyna_xlsx/12292277 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2020.00561.s001 |
_version_ |
1766330097767284736 |