Table_1_Deriving a Proxy for Iron Limitation From Chlorophyll Fluorescence on Buoyancy Gliders.DOCX

• Main Authors: Thomas J. Ryan-Keogh, Sandy J. Thomalla
• Format: Dataset
• Language: unknown
• Published: 2020
• Subjects: Oceanography; Marine Biology; Marine Geoscience; Biological Oceanography; Chemical Oceanography; Physical Oceanography; Marine Engineering; iron; fluorescence; gliders; chlorophyll; non-photochemical chlorophyll fluorescence quenching; Antarctic; Southern Ocean; Antarc*
• Online Access: https://doi.org/10.3389/fmars.2020.00275.s001; https://figshare.com/articles/Table_1_Deriving_a_Proxy_for_Iron_Limitation_From_Chlorophyll_Fluorescence_on_Buoyancy_Gliders_DOCX/12247511

Chlorophyll fluorescence, primarily used to derive phytoplankton biomass, has long been an underutilized source of information on phytoplankton physiology. Diel fluctuations in chlorophyll fluorescence are affected by both photosynthetic efficiency and non-photochemical quenching (NPQ), where NPQ is a decrease in fluorescence through the dissipation of excess energy as heat. NPQ variability is linked to iron and light availability, and has the potential to provide important diagnostic information on phytoplankton physiology. Here we establish a relationship between NPQ sv (Stern-Volmer NPQ) and indices of iron limitation from nutrient addition experiments in the sub-Antarctic zone (SAZ) of the Atlantic Southern Ocean, through the derivation of NPQ max (the maximum NPQ sv value) and α NPQ (the light limited slope of NPQ sv ). Significant differences were found for both F v /F m and α NPQ for iron versus control treatments, with no significant differences for NPQ max . Similar results from CTDs indicated that changes in NPQ were driven by increasing light availability from late July to December, but by both iron and light from January to February. We propose here that variability in α NPQ , which has removed the effect of light availability, can potentially be used as a proxy for iron limitation (as shown here for the Atlantic SAZ), with higher values being associated with greater iron stress. This approach was transferred to data from a buoyancy glider deployment at the same location by utilizing the degree of fluorescence quenching as a proxy for NPQ G lider , which was plotted against in situ light to determine α NPQ. Seasonal increases in α NPQ are consistent with increased light availability, shoaling of the mixed layer depth (MLD) and anticipated seasonal iron limitation. The transition from winter to summer, when positive net heat flux dominates stratification, was coincident with a 24% increase in α NPQ variability and a switch in the dominant driver from incident PAR to MLD. The dominant scales of α NPQ ...