Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG
Molluscs have evolved a primitive but complete neuroendocrine-immune (NEI) system with a vast array of neurotransmitters to conduct both humoral and cellular immunomodulation. Previous studies have illustrated the immune functions of several key neurotransmitters. However, the combined effects of mu...
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ftfrontimediafig:oai:figshare.com:article/12142920 2023-05-15T15:58:24+02:00 Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG Zhaoqun Liu Zhi Zhou Lingling Wang Yukun Zhang Yanan Zong Yan Zheng Meijia Li Weilin Wang Linsheng Song 2020-04-17T04:23:59Z https://doi.org/10.3389/fimmu.2020.00616.s003 https://figshare.com/articles/Image_3_A_Signaling_Pathway_to_Mediate_the_Combined_Immunomodulation_of_Acetylcholine_and_Enkephalin_in_Oyster_Crassostrea_gigas_JPEG/12142920 unknown doi:10.3389/fimmu.2020.00616.s003 https://figshare.com/articles/Image_3_A_Signaling_Pathway_to_Mediate_the_Combined_Immunomodulation_of_Acetylcholine_and_Enkephalin_in_Oyster_Crassostrea_gigas_JPEG/12142920 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas hemocyte neurotransmitter immune regulation signaling pathway Image Figure 2020 ftfrontimediafig https://doi.org/10.3389/fimmu.2020.00616.s003 2020-04-22T22:54:38Z Molluscs have evolved a primitive but complete neuroendocrine-immune (NEI) system with a vast array of neurotransmitters to conduct both humoral and cellular immunomodulation. Previous studies have illustrated the immune functions of several key neurotransmitters. However, the combined effects of multiple neurotransmitters and the signaling pathway to mediate such immunomodulation have not been well-understood. In the present study, iTRAQ and LC-ESI-MS/MS approaches were employed to investigate the combined immunomodulation functions of two crucial neurotransmitters, acetylcholine (ACh), and [Met 5 ]-enkephalin (ENK), in oyster Crassostrea gigas. A total number of 5,379 proteins were identified from hemocytes of oysters after the treatments with Ach and ENK separately or simultaneously, and 1,475 of them were found to be significantly up-regulated, while 1,115 of them were significantly down-regulated. The protein expression patterns in the groups treated by ACh and ENK separately were quite similar, which were dramatically different from that in the group treated by ACh+ENK. One hundred seventy-two proteins were found to be differentially expressed in all the three neurotransmitter treatment groups. Functional validation suggested that ACh and ENK possibly modulate the immune response in oyster hemocytes by enhancing pathogen recognition, cell apoptosis, and the enzyme activities of superoxide dismutase (SOD). Moreover, GO enrichment and co-expression network analyses implied that the combined immunomodulation of ACh and ENK might be mediated by p53, EGF-R–ErbB, and Fc gamma R (FcγR) signaling pathways. These results collectively indicated that multiple neurotransmitters executed a combined and ordered immune regulation through common signaling cascades in molluscs, which was under delicate control to maintain the homeostasis. Still Image Crassostrea gigas Frontiers: Figshare |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas hemocyte neurotransmitter immune regulation signaling pathway |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas hemocyte neurotransmitter immune regulation signaling pathway Zhaoqun Liu Zhi Zhou Lingling Wang Yukun Zhang Yanan Zong Yan Zheng Meijia Li Weilin Wang Linsheng Song Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas hemocyte neurotransmitter immune regulation signaling pathway |
description |
Molluscs have evolved a primitive but complete neuroendocrine-immune (NEI) system with a vast array of neurotransmitters to conduct both humoral and cellular immunomodulation. Previous studies have illustrated the immune functions of several key neurotransmitters. However, the combined effects of multiple neurotransmitters and the signaling pathway to mediate such immunomodulation have not been well-understood. In the present study, iTRAQ and LC-ESI-MS/MS approaches were employed to investigate the combined immunomodulation functions of two crucial neurotransmitters, acetylcholine (ACh), and [Met 5 ]-enkephalin (ENK), in oyster Crassostrea gigas. A total number of 5,379 proteins were identified from hemocytes of oysters after the treatments with Ach and ENK separately or simultaneously, and 1,475 of them were found to be significantly up-regulated, while 1,115 of them were significantly down-regulated. The protein expression patterns in the groups treated by ACh and ENK separately were quite similar, which were dramatically different from that in the group treated by ACh+ENK. One hundred seventy-two proteins were found to be differentially expressed in all the three neurotransmitter treatment groups. Functional validation suggested that ACh and ENK possibly modulate the immune response in oyster hemocytes by enhancing pathogen recognition, cell apoptosis, and the enzyme activities of superoxide dismutase (SOD). Moreover, GO enrichment and co-expression network analyses implied that the combined immunomodulation of ACh and ENK might be mediated by p53, EGF-R–ErbB, and Fc gamma R (FcγR) signaling pathways. These results collectively indicated that multiple neurotransmitters executed a combined and ordered immune regulation through common signaling cascades in molluscs, which was under delicate control to maintain the homeostasis. |
format |
Still Image |
author |
Zhaoqun Liu Zhi Zhou Lingling Wang Yukun Zhang Yanan Zong Yan Zheng Meijia Li Weilin Wang Linsheng Song |
author_facet |
Zhaoqun Liu Zhi Zhou Lingling Wang Yukun Zhang Yanan Zong Yan Zheng Meijia Li Weilin Wang Linsheng Song |
author_sort |
Zhaoqun Liu |
title |
Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG |
title_short |
Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG |
title_full |
Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG |
title_fullStr |
Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG |
title_full_unstemmed |
Image_3_A Signaling Pathway to Mediate the Combined Immunomodulation of Acetylcholine and Enkephalin in Oyster Crassostrea gigas.JPEG |
title_sort |
image_3_a signaling pathway to mediate the combined immunomodulation of acetylcholine and enkephalin in oyster crassostrea gigas.jpeg |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.00616.s003 https://figshare.com/articles/Image_3_A_Signaling_Pathway_to_Mediate_the_Combined_Immunomodulation_of_Acetylcholine_and_Enkephalin_in_Oyster_Crassostrea_gigas_JPEG/12142920 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
doi:10.3389/fimmu.2020.00616.s003 https://figshare.com/articles/Image_3_A_Signaling_Pathway_to_Mediate_the_Combined_Immunomodulation_of_Acetylcholine_and_Enkephalin_in_Oyster_Crassostrea_gigas_JPEG/12142920 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.00616.s003 |
_version_ |
1766394147750543360 |