Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF
Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess en...
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ftfrontimediafig:oai:figshare.com:article/12127917 2023-05-15T18:18:48+02:00 Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF Lander Blommaert Emmelien Vancaester Marie J. J. Huysman Cristina M. Osuna-Cruz Sofie D’hondt Johann Lavaud Bernard Lepetit Per Winge Atle M. Bones Klaas Vandepoele Wim Vyverman Koen Sabbe 2020-04-15T04:28:09Z https://doi.org/10.3389/fmars.2020.00192.s001 https://figshare.com/articles/Data_Sheet_1_Light_Regulation_of_LHCX_Genes_in_the_Benthic_Diatom_Seminavis_robusta_PDF/12127917 unknown doi:10.3389/fmars.2020.00192.s001 https://figshare.com/articles/Data_Sheet_1_Light_Regulation_of_LHCX_Genes_in_the_Benthic_Diatom_Seminavis_robusta_PDF/12127917 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering diatom microphytobenthos light stress LHCX physiology Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmars.2020.00192.s001 2020-04-15T22:53:28Z Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess energy. Its capacity is mainly defined by the xanthophyll cycle (XC) and Light-Harvesting Complex X (LHCX) proteins. Whereas the XC and its relation to NPQ have been relatively well-studied in both planktonic and benthic diatoms, our current knowledge about LHCX proteins and their potential involvement in NPQ regulation is largely restricted to planktonic diatoms. While recent studies using immuno-blotting have revealed the presence of light regulated LHCX proteins in benthic diatom communities and isolates, nothing is as yet known about the diversity, identity and transcriptional regulation or function of these proteins. We identified LHCX genes in the draft genome of the model benthic diatom Seminavis robusta and followed their transcriptional regulation during a day/night cycle and during exposure to high light conditions. The S. robusta genome contains 17 LHCX sequences, which is much more than in the sequenced planktonic model diatoms (4–5), but similar to the number of LHCX genes in the sea ice associated diatom Fragilariopsis cylindrus. LHCX diversification in both species, however, appears to have occurred independently. Interestingly, the S. robusta genome contains LHCX genes that are related to the LHCX6 of the model centric diatom Thalassiosira pseudonana, which are lacking in the well-studied pennate model diatom Phaeodactylum tricornutum. All investigated LHCX genes, with exception of SrLHCX6, were upregulated during the daily dark-light transition. Exposure to 2,000 μmol photons m –2 s –1 , furthermore, increased transcription of all investigated LHCX genes. Our data suggest that the diversification and involvement of several light regulated LHCX genes in the photophysiology of S. robusta may represent an ... Dataset Sea ice Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering diatom microphytobenthos light stress LHCX physiology |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering diatom microphytobenthos light stress LHCX physiology Lander Blommaert Emmelien Vancaester Marie J. J. Huysman Cristina M. Osuna-Cruz Sofie D’hondt Johann Lavaud Bernard Lepetit Per Winge Atle M. Bones Klaas Vandepoele Wim Vyverman Koen Sabbe Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering diatom microphytobenthos light stress LHCX physiology |
description |
Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess energy. Its capacity is mainly defined by the xanthophyll cycle (XC) and Light-Harvesting Complex X (LHCX) proteins. Whereas the XC and its relation to NPQ have been relatively well-studied in both planktonic and benthic diatoms, our current knowledge about LHCX proteins and their potential involvement in NPQ regulation is largely restricted to planktonic diatoms. While recent studies using immuno-blotting have revealed the presence of light regulated LHCX proteins in benthic diatom communities and isolates, nothing is as yet known about the diversity, identity and transcriptional regulation or function of these proteins. We identified LHCX genes in the draft genome of the model benthic diatom Seminavis robusta and followed their transcriptional regulation during a day/night cycle and during exposure to high light conditions. The S. robusta genome contains 17 LHCX sequences, which is much more than in the sequenced planktonic model diatoms (4–5), but similar to the number of LHCX genes in the sea ice associated diatom Fragilariopsis cylindrus. LHCX diversification in both species, however, appears to have occurred independently. Interestingly, the S. robusta genome contains LHCX genes that are related to the LHCX6 of the model centric diatom Thalassiosira pseudonana, which are lacking in the well-studied pennate model diatom Phaeodactylum tricornutum. All investigated LHCX genes, with exception of SrLHCX6, were upregulated during the daily dark-light transition. Exposure to 2,000 μmol photons m –2 s –1 , furthermore, increased transcription of all investigated LHCX genes. Our data suggest that the diversification and involvement of several light regulated LHCX genes in the photophysiology of S. robusta may represent an ... |
format |
Dataset |
author |
Lander Blommaert Emmelien Vancaester Marie J. J. Huysman Cristina M. Osuna-Cruz Sofie D’hondt Johann Lavaud Bernard Lepetit Per Winge Atle M. Bones Klaas Vandepoele Wim Vyverman Koen Sabbe |
author_facet |
Lander Blommaert Emmelien Vancaester Marie J. J. Huysman Cristina M. Osuna-Cruz Sofie D’hondt Johann Lavaud Bernard Lepetit Per Winge Atle M. Bones Klaas Vandepoele Wim Vyverman Koen Sabbe |
author_sort |
Lander Blommaert |
title |
Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF |
title_short |
Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF |
title_full |
Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF |
title_fullStr |
Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF |
title_full_unstemmed |
Data_Sheet_1_Light Regulation of LHCX Genes in the Benthic Diatom Seminavis robusta.PDF |
title_sort |
data_sheet_1_light regulation of lhcx genes in the benthic diatom seminavis robusta.pdf |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmars.2020.00192.s001 https://figshare.com/articles/Data_Sheet_1_Light_Regulation_of_LHCX_Genes_in_the_Benthic_Diatom_Seminavis_robusta_PDF/12127917 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_relation |
doi:10.3389/fmars.2020.00192.s001 https://figshare.com/articles/Data_Sheet_1_Light_Regulation_of_LHCX_Genes_in_the_Benthic_Diatom_Seminavis_robusta_PDF/12127917 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2020.00192.s001 |
_version_ |
1766195511098867712 |