Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX
Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the ful...
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ftfrontimediafig:oai:figshare.com:article/11879997 2023-05-15T16:57:03+02:00 Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen 2020-02-21T04:08:59Z https://doi.org/10.3389/fmicb.2020.00249.s007 https://figshare.com/articles/Table_4_Tailoring_Hydrothermal_Vent_Biodiversity_Toward_Improved_Biodiscovery_Using_a_Novel_in_situ_Enrichment_Strategy_DOCX/11879997 unknown doi:10.3389/fmicb.2020.00249.s007 https://figshare.com/articles/Table_4_Tailoring_Hydrothermal_Vent_Biodiversity_Toward_Improved_Biodiscovery_Using_a_Novel_in_situ_Enrichment_Strategy_DOCX/11879997 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Dataset 2020 ftfrontimediafig https://doi.org/10.3389/fmicb.2020.00249.s007 2020-02-26T23:53:50Z Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme ... Dataset Jan Mayen Frontiers: Figshare Jan Mayen |
institution |
Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology deep sea hydrothermal sediments in situ enrichment marine bioprospecting biotechnology |
description |
Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme ... |
format |
Dataset |
author |
Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen |
author_facet |
Runar Stokke Eoghan P. Reeves Håkon Dahle Anita-Elin Fedøy Thomas Viflot Solveig Lie Onstad Francesca Vulcano Rolf B. Pedersen Vincent G. H. Eijsink Ida H. Steen |
author_sort |
Runar Stokke |
title |
Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX |
title_short |
Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX |
title_full |
Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX |
title_fullStr |
Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX |
title_full_unstemmed |
Table_4_Tailoring Hydrothermal Vent Biodiversity Toward Improved Biodiscovery Using a Novel in situ Enrichment Strategy.DOCX |
title_sort |
table_4_tailoring hydrothermal vent biodiversity toward improved biodiscovery using a novel in situ enrichment strategy.docx |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00249.s007 https://figshare.com/articles/Table_4_Tailoring_Hydrothermal_Vent_Biodiversity_Toward_Improved_Biodiscovery_Using_a_Novel_in_situ_Enrichment_Strategy_DOCX/11879997 |
geographic |
Jan Mayen |
geographic_facet |
Jan Mayen |
genre |
Jan Mayen |
genre_facet |
Jan Mayen |
op_relation |
doi:10.3389/fmicb.2020.00249.s007 https://figshare.com/articles/Table_4_Tailoring_Hydrothermal_Vent_Biodiversity_Toward_Improved_Biodiscovery_Using_a_Novel_in_situ_Enrichment_Strategy_DOCX/11879997 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.00249.s007 |
_version_ |
1766048318894374912 |