Data_Sheet_5_Dilution of Seawater Affects the Ca2 + Transport in the Outer Mantle Epithelium of Crassostrea gigas.PDF

• Main Authors: J. Kirsikka Sillanpää, Joao Carlos dos Reis Cardoso, Rute Castelo Félix, Liliana Anjos, Deborah Mary Power, Kristina Sundell
• Format: Dataset
• Language: unknown
• Published: 2020
• Subjects: Physiology; Exercise Physiology; Nutritional Physiology; Reproduction; Cell Physiology; Systems Physiology; Animal Physiology - Biophysics; Animal Physiology - Cell; Animal Physiology - Systems; Comparative Physiology; Physiology not elsewhere classified; Ca2+-ATPase; Na+/Ca2+-exchanger; calcium channel; Na+/K+-ATPase; calcification; salinity; Crassostrea gigas
• Online Access: https://doi.org/10.3389/fphys.2020.00001.s005; https://figshare.com/articles/Data_Sheet_5_Dilution_of_Seawater_Affects_the_Ca2_Transport_in_the_Outer_Mantle_Epithelium_of_Crassostrea_gigas_PDF/11680749

Varying salinities of coastal waters are likely to affect the physiology and ion transport capabilities of calcifying marine organisms such as bivalves. To investigate the physiological effect of decreased environmental salinity in bivalves, adult oysters (Crassostrea gigas) were exposed for 14 days to 50% seawater (14) and the effects on mantle ion transport, electrophysiology and the expression of Ca 2+ transporters and channels relative to animals maintained in full strength sea water (28) was evaluated. Exposure of oysters to a salinity of 14 decreased the active mantle transepithelial ion transport and specifically affected Ca 2+ transfer. Gene expression of the Na + /K + -ATPase and the sarco(endo)plasmic reticulum Ca 2+ -ATPase was decreased whereas the expression of the T-type voltage-gated Ca channel and the Na + /Ca 2+ -exchanger increased compared to animals maintained in full SW. The results indicate that decreased environmental salinities will most likely affect not only osmoregulation but also bivalve biomineralization and shell formation.