Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf

• Main Authors: Javier Sánchez Romano, Lisa Grund, Anna Obiegala, Ingebjørg H. Nymo, Francisco Javier Ancin-Murguzur, Hong Li, Nina Król, Martin Pfeffer, Morten Tryland
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Animal Systematics and Taxonomy; Animal Physiology - Biophysics; Animal Physiology - Cell; Animal Physiology - Systems; Animal Behaviour; Animal Cell and Molecular Biology; Animal Developmental and Reproductive Biology; Animal Immunology; Animal Neurobiology; Animal Physiological Ecology; Animal Structure and Function; Veterinary Anaesthesiology and Intensive Care; Veterinary Anatomy and Physiology; Veterinary Diagnosis and Diagnostics; Veterinary Epidemiology; Veterinary Immunology; Veterinary Medicine; Veterinary Microbiology (excl. Virology); Veterinary Parasitology; Veterinary Pathology; Veterinary Pharmacology; Veterinary Surgery; Veterinary Virology; Veterinary Sciences not elsewhere classified; herpesvirus; pestivirus; Schmallenberg virus; bluetongue virus; Brucella; Toxoplasma gondii; Neospora caninum; Anaplasma phagocytophilum; Rangifer tarandus
• Online Access: https://doi.org/10.3389/fvets.2019.00461.s001; https://figshare.com/articles/Data_Sheet_1_A_Multi-Pathogen_Screening_of_Captive_Reindeer_Rangifer_tarandus_in_Germany_Based_on_Serological_and_Molecular_Assays_pdf/11417439

Captive reindeer in German zoos and wildlife parks live outside their natural geographic range and are exposed to a variety of viral, bacterial and protozoan pathogens, some host-specific and some which they are not exposed to in their native habitat. Reindeer blood samples and ticks collected in 2013 from 123 reindeer at 16 different zoological facilities were available from a previous study. The aims of this study were to assess the serological status of these animals with regards to various microorganisms as well as to test ticks (Ixodes ricinus) and blood samples for the presence of Anaplasma spp. DNA in order to evaluate the exposure of captive reindeer in Germany to a variety of pathogens. A total of 119 or 118 serum samples were screened (ELISA) and antibodies were detected (seropositive/tested, prevalence, confidence interval) against alphaherpesvirus (24/119, 20.3%, CI: 13.9–28.3), bluetongue virus (BTV; 4/119, 3.4%, CI: 1.0–8.7), malignant catarrhal fever related gammaherpesvirus (MCFV-related gammaherpesvirus; 7/119, 5.9%, CI: 2.7–11.9), pestivirus (5/118, 4.2%, CI: 1.6–9.8), Schmallenberg virus (SBV; 70/118, 59.3%, CI: 50.3–67.8), smooth Brucella spp. (1/118; 0.9%, CI: 0–5.1), Neospora caninum (5/118, 4.2%, CI: 1.6–9.8), and Toxoplasma gondii (62/119, 52.1%, CI: 43.2–60.9). These results suggested the exposure of reindeer to all tested pathogens. Moreover, real-time PCR for Anaplasma phagocytophilum targeting the partial msp2 gene was performed on DNA extracted from whole blood samples from reindeer (n = 123) and from ticks (n = 49) collected from 22 reindeer in seven different facilities. In addition to the real-time PCR, a semi-nested PCR for the partial groEL gene, and a nested PCR targeting the partial 16S rRNA gene were performed. DNA of A. phagocytophilum was detected in 17 reindeer (13.8%) and 15 ticks (30.6%). Three of the five reindeer with ticks having A. phagocytophilum DNA also had such DNA in blood. These results indicate that captive reindeer can be exposed to several ruminant pathogens ...