Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf
Captive reindeer in German zoos and wildlife parks live outside their natural geographic range and are exposed to a variety of viral, bacterial and protozoan pathogens, some host-specific and some which they are not exposed to in their native habitat. Reindeer blood samples and ticks collected in 20...
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ftfrontimediafig:oai:figshare.com:article/11417439 2023-05-15T18:04:27+02:00 Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf Javier Sánchez Romano Lisa Grund Anna Obiegala Ingebjørg H. Nymo Francisco Javier Ancin-Murguzur Hong Li Nina Król Martin Pfeffer Morten Tryland 2019-12-20T12:21:45Z https://doi.org/10.3389/fvets.2019.00461.s001 https://figshare.com/articles/Data_Sheet_1_A_Multi-Pathogen_Screening_of_Captive_Reindeer_Rangifer_tarandus_in_Germany_Based_on_Serological_and_Molecular_Assays_pdf/11417439 unknown doi:10.3389/fvets.2019.00461.s001 https://figshare.com/articles/Data_Sheet_1_A_Multi-Pathogen_Screening_of_Captive_Reindeer_Rangifer_tarandus_in_Germany_Based_on_Serological_and_Molecular_Assays_pdf/11417439 CC BY 4.0 CC-BY Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified herpesvirus pestivirus Schmallenberg virus bluetongue virus Brucella Toxoplasma gondii Neospora caninum Anaplasma phagocytophilum Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fvets.2019.00461.s001 2019-12-25T23:50:32Z Captive reindeer in German zoos and wildlife parks live outside their natural geographic range and are exposed to a variety of viral, bacterial and protozoan pathogens, some host-specific and some which they are not exposed to in their native habitat. Reindeer blood samples and ticks collected in 2013 from 123 reindeer at 16 different zoological facilities were available from a previous study. The aims of this study were to assess the serological status of these animals with regards to various microorganisms as well as to test ticks (Ixodes ricinus) and blood samples for the presence of Anaplasma spp. DNA in order to evaluate the exposure of captive reindeer in Germany to a variety of pathogens. A total of 119 or 118 serum samples were screened (ELISA) and antibodies were detected (seropositive/tested, prevalence, confidence interval) against alphaherpesvirus (24/119, 20.3%, CI: 13.9–28.3), bluetongue virus (BTV; 4/119, 3.4%, CI: 1.0–8.7), malignant catarrhal fever related gammaherpesvirus (MCFV-related gammaherpesvirus; 7/119, 5.9%, CI: 2.7–11.9), pestivirus (5/118, 4.2%, CI: 1.6–9.8), Schmallenberg virus (SBV; 70/118, 59.3%, CI: 50.3–67.8), smooth Brucella spp. (1/118; 0.9%, CI: 0–5.1), Neospora caninum (5/118, 4.2%, CI: 1.6–9.8), and Toxoplasma gondii (62/119, 52.1%, CI: 43.2–60.9). These results suggested the exposure of reindeer to all tested pathogens. Moreover, real-time PCR for Anaplasma phagocytophilum targeting the partial msp2 gene was performed on DNA extracted from whole blood samples from reindeer (n = 123) and from ticks (n = 49) collected from 22 reindeer in seven different facilities. In addition to the real-time PCR, a semi-nested PCR for the partial groEL gene, and a nested PCR targeting the partial 16S rRNA gene were performed. DNA of A. phagocytophilum was detected in 17 reindeer (13.8%) and 15 ticks (30.6%). Three of the five reindeer with ticks having A. phagocytophilum DNA also had such DNA in blood. These results indicate that captive reindeer can be exposed to several ruminant pathogens ... Dataset Rangifer tarandus Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified herpesvirus pestivirus Schmallenberg virus bluetongue virus Brucella Toxoplasma gondii Neospora caninum Anaplasma phagocytophilum |
spellingShingle |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified herpesvirus pestivirus Schmallenberg virus bluetongue virus Brucella Toxoplasma gondii Neospora caninum Anaplasma phagocytophilum Javier Sánchez Romano Lisa Grund Anna Obiegala Ingebjørg H. Nymo Francisco Javier Ancin-Murguzur Hong Li Nina Król Martin Pfeffer Morten Tryland Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf |
topic_facet |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified herpesvirus pestivirus Schmallenberg virus bluetongue virus Brucella Toxoplasma gondii Neospora caninum Anaplasma phagocytophilum |
description |
Captive reindeer in German zoos and wildlife parks live outside their natural geographic range and are exposed to a variety of viral, bacterial and protozoan pathogens, some host-specific and some which they are not exposed to in their native habitat. Reindeer blood samples and ticks collected in 2013 from 123 reindeer at 16 different zoological facilities were available from a previous study. The aims of this study were to assess the serological status of these animals with regards to various microorganisms as well as to test ticks (Ixodes ricinus) and blood samples for the presence of Anaplasma spp. DNA in order to evaluate the exposure of captive reindeer in Germany to a variety of pathogens. A total of 119 or 118 serum samples were screened (ELISA) and antibodies were detected (seropositive/tested, prevalence, confidence interval) against alphaherpesvirus (24/119, 20.3%, CI: 13.9–28.3), bluetongue virus (BTV; 4/119, 3.4%, CI: 1.0–8.7), malignant catarrhal fever related gammaherpesvirus (MCFV-related gammaherpesvirus; 7/119, 5.9%, CI: 2.7–11.9), pestivirus (5/118, 4.2%, CI: 1.6–9.8), Schmallenberg virus (SBV; 70/118, 59.3%, CI: 50.3–67.8), smooth Brucella spp. (1/118; 0.9%, CI: 0–5.1), Neospora caninum (5/118, 4.2%, CI: 1.6–9.8), and Toxoplasma gondii (62/119, 52.1%, CI: 43.2–60.9). These results suggested the exposure of reindeer to all tested pathogens. Moreover, real-time PCR for Anaplasma phagocytophilum targeting the partial msp2 gene was performed on DNA extracted from whole blood samples from reindeer (n = 123) and from ticks (n = 49) collected from 22 reindeer in seven different facilities. In addition to the real-time PCR, a semi-nested PCR for the partial groEL gene, and a nested PCR targeting the partial 16S rRNA gene were performed. DNA of A. phagocytophilum was detected in 17 reindeer (13.8%) and 15 ticks (30.6%). Three of the five reindeer with ticks having A. phagocytophilum DNA also had such DNA in blood. These results indicate that captive reindeer can be exposed to several ruminant pathogens ... |
format |
Dataset |
author |
Javier Sánchez Romano Lisa Grund Anna Obiegala Ingebjørg H. Nymo Francisco Javier Ancin-Murguzur Hong Li Nina Król Martin Pfeffer Morten Tryland |
author_facet |
Javier Sánchez Romano Lisa Grund Anna Obiegala Ingebjørg H. Nymo Francisco Javier Ancin-Murguzur Hong Li Nina Król Martin Pfeffer Morten Tryland |
author_sort |
Javier Sánchez Romano |
title |
Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf |
title_short |
Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf |
title_full |
Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf |
title_fullStr |
Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf |
title_full_unstemmed |
Data_Sheet_1_A Multi-Pathogen Screening of Captive Reindeer (Rangifer tarandus) in Germany Based on Serological and Molecular Assays.pdf |
title_sort |
data_sheet_1_a multi-pathogen screening of captive reindeer (rangifer tarandus) in germany based on serological and molecular assays.pdf |
publishDate |
2019 |
url |
https://doi.org/10.3389/fvets.2019.00461.s001 https://figshare.com/articles/Data_Sheet_1_A_Multi-Pathogen_Screening_of_Captive_Reindeer_Rangifer_tarandus_in_Germany_Based_on_Serological_and_Molecular_Assays_pdf/11417439 |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_relation |
doi:10.3389/fvets.2019.00461.s001 https://figshare.com/articles/Data_Sheet_1_A_Multi-Pathogen_Screening_of_Captive_Reindeer_Rangifer_tarandus_in_Germany_Based_on_Serological_and_Molecular_Assays_pdf/11417439 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fvets.2019.00461.s001 |
_version_ |
1766175835455225856 |