Table_1_De novo Assembly and Characterization of Patagonian Toothfish Transcriptome and Develop of EST-SSR Markers for Population Genetics.XLSX

• Main Authors: Jorge Touma, Killen Ko García, Scarleth Bravo, Francisco Leiva, Javier Moya, Luis Vargas-Chacoff, Alberto Reyes, Rodrigo Vidal
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Oceanography; Marine Biology; Marine Geoscience; Biological Oceanography; Chemical Oceanography; Physical Oceanography; Marine Engineering; Patagonian toothfish; NGS; transcriptome; EST-SSR; genetic diversity; Antarctic; Pacific; Antarc*; Patagonian Toothfish
• Online Access: https://doi.org/10.3389/fmars.2019.00720.s001; https://figshare.com/articles/Table_1_De_novo_Assembly_and_Characterization_of_Patagonian_Toothfish_Transcriptome_and_Develop_of_EST-SSR_Markers_for_Population_Genetics_XLSX/11296100

Patagonian toothfish (Dissostichus eleginoides) is a hefty notothenioid fish and a key species within the marine ecosystem with a high migratory capacity across sub-Antarctic and south American Pacific oceans. Transcriptome characterization and molecular markers associated with micro and macro-evolutionary studies are not available, which in turn limits the gain of knowledge about the genetic basis of this species. Therefore, in the present study, a de novo transcriptome from eight tissue and an embryonic state of Patagonian toothfish was developed, using the Illumina Hiseq 4000 platform. A total of 233,424 superTranscripts were assembled and 37,446 annotated against public databases. Moreover, we identified 71,107 expressed sequence tag-simple sequence repeats (EST-SSRs), with an average number of 0.3 SSRs per superTranscripts and one SSR per 1.12 kB. The most abundant SSR type was repeated dinucleotide (53.67%), followed by trinucleotide (13.73%) repeats. From the total of EST-SSRs identified, 34,196 primer pairs were properly designed and a subset of 25 immune loci were selected for its evaluation as potential EST-SSR population markers. Of this subset, 11 proved to have good technical features and were evaluated in 64 animals from four Patagonian toothfish populations. A number of 63 alleles were identified, with a mean of 4.9 alleles per locus and a polymorphism information content ranging from 0.224 to 0.591, with a mean of 0.50. Significant (F ST , range 0.082–0.117 and GST′, range 0.069–0.291) genetic differentiation (P < 0.05) was determined among the populations analyzed. Therefore, the results presented here represent a relevant genetic resource for biological studies on evolution, conservation, genetic diversity, population structure, and genetic management of breeding stocks of this important species.